Recent applications of the derivatization techniques in capillary electrophoresis

Gotti, Roberto; Pasquini, Benedetta; Orlandini, Serena; Furlanetto, Sandra

doi:10.1016/j.jpbao.2023.100003

Cited by 4 publications

(2 citation statements)

References 96 publications

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“…This is explained as the voltage is involved in the derivatisation of digoxin and the preconcentration of the species. 25,26 Surface responses of 2-NBA and o-TBA are presented in Fig. 2 and 3, respectively.…”

Section: Papermentioning

confidence: 99%

Evaluation of digoxin-boronate ester formation through in-capillary derivatisation-large volume sample stacking-capillary zone electrophoresis

Muñoz,

Alvarez-Hernandez,

Ibarra

et al. 2024

Anal. Methods

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Section: Papermentioning

confidence: 99%

Evaluation of digoxin-boronate ester formation through in-capillary derivatisation-large volume sample stacking-capillary zone electrophoresis

Muñoz,

Alvarez-Hernandez,

Ibarra

et al. 2024

Anal. Methods

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“…The in‐capillary simultaneous fluorescent and chiral derivatization of AAS by ortho‐phthalaldehyde (fluorescent label) and N ‐acetyl‐ l ‐cysteine (chiral agent) [106] can be applied also for peptides. For other examples of precapillary, in‐line, in‐capillary, and postcapillary derivatization for the separation and detection of variable compounds, including peptides in CE with UV, LIF, and MS detection, see the recent review [107].…”

Section: Sample Preparationmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Determination of amino acids and peptides without their pre‐column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview

Kartsova,

Maliushevska

2024

J of Separation Science

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This review provides an overview of recent works focusing on the determination of amino acids (AAs) and peptides using capillary electrophoresis with contactless conductivity detection and ultraviolet (UV) detection, which is the most widespread detection in capillary electromigration techniques, without pre‐capillary derivatization. Available options for the UV detection of these analytes, such as indirect detection, complexation with transition metal ions, and in‐capillary derivatization are described. Developments in the field of direct detection of UV‐absorbing AAs and peptides as well as progress in chiral separation are described. A separate section is dedicated to using on‐line sample preconcentration methods combined with capillary electrophoresis‐UV.

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Recent applications of the derivatization techniques in capillary electrophoresis

Cited by 4 publications

References 96 publications

Evaluation of digoxin-boronate ester formation through in-capillary derivatisation-large volume sample stacking-capillary zone electrophoresis

Evaluation of digoxin-boronate ester formation through in-capillary derivatisation-large volume sample stacking-capillary zone electrophoresis

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Determination of amino acids and peptides without their pre‐column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview

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