Recent Approaches to the Manufacturing of Biomimetic Multi-Phasic Scaffolds for Osteochondral Regeneration

Longley, Ryan; Ferreira, Ana Marina; Gentile, Piergiorgio

doi:10.3390/ijms19061755

Cited by 47 publications

(47 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Assessing ECM composition and architecture using high-resolution confocal microscopy enabled elucidation of a highly resolved spatial distribution and regional variation in collagen architecture not previously reported for an entire rudiment. Previous immunofluorescence studies have localised collagens I, II, III, VI, IX and X in the embryonic limb (Archer et al, 1994;Castagnola et al, 1988;Duance et al, 1982;Evans et al, 1983;Foolen et al, 2008;Hartmann et al, 1983;Hering et al, 2014;Irwin et al, 1985;Kong et al, 1993;Kwan et al, 1991;Lewis et al, 2012;LuValle et al, 1992;Mendler et al, 1989;Morrison et al, 1996;Muller-Glauser et al, 1986;Oshima et al, 1989;Poole et al, 1984;Ricard-Blum et al, 1982;Schmid and Linsenmayer, 1985;Shen, 2005;Shoham et al, 2016;Treilleux et al, 1992;Vornehm et al, 1996;Wilusz et al, 2012) but only a few studies (Shoham et al, 2016;Wilusz et al, 2012) have analysed some of these collagen structures using the improved resolution offered by confocal microscopy. The main novelty of the present study is that it revealed, for the first time, to the authors' knowledge, the emergence and maturation of key cartilage and bone collagens, in high resolution, at key locations across the entire rudiment, including the joint regions, at three of the most developmentally significant stages of skeletogenesis.…”

Section: Discussionmentioning

confidence: 99%

Initiation and emerging complexity of the collagen network during prenatal skeletal development

Ahmed

Nowlan²

2020

eCM

View full text Add to dashboard Cite

The establishment of a complex collagen network is critical for the architecture and mechanical properties of cartilage and bone. However, when and how the key collagens in cartilage and bone develop has not been characterised in detail. The study provides a detailed qualitative characterisation of the spatial localisations of collagens I-III, V-VI and IX-XI in the mouse and their regional architecture variation over three developmentally significant time points: when the rudiment starts to form at E13.5 [Theiler stage (TS) 22], when mineralisation is present at E16.5 (TS25) and during the latest prenatal stage at E18.5 (TS27). Dynamic changes in collagen distribution between stages with the progression of the growth plate and mineralisation (particularly collagens I, II, V, X and XI) and dramatic changes in collagen structural organisation and complexity with maturation, especially for collagens II and XI, were observed. The future articular cartilage region was demarcated by pronounced collagens II and VI expression at TS27 and the emergence of collagens I, III, V, IX and XI in the tendon and its insertion site was observed. The present study revealed, for the first time, the emergence and maturation of key cartilage and bone collagens, in high resolution, at multiple locations across the entire rudiment, including the joint regions, at three of the most developmentally significant stages of skeletogenesis, furthering the understanding of disease and regeneration of skeletal tissues.

show abstract

Section: Discussionmentioning

confidence: 99%

Initiation and emerging complexity of the collagen network during prenatal skeletal development

Ahmed

Nowlan²

2020

eCM

View full text Add to dashboard Cite

show abstract

“…Attempts have been made by developing biphasic scaffolds that have an osseous layer providing rigid, structural support incorporated to a more bioactive chondral layer into which cells may be seeded. It has been postulated that a triphasic scaffold with an intermediate layer between the chondral and bone layers would be beneficial and emulate the tidemark found in a native osteochondral unit (Marquass et al, 2010;Longley et al, 2018). This intermediate layer would have to mimic the osteochondral junction containing intricate networks of arteries and nerves with no currently available faultless biomaterial.…”

Section: Regenerative Techniquesmentioning

confidence: 99%

Osteochondral Injury, Management and Tissue Engineering Approaches

Jacob

Shimomura

Nakamura

2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Osteochondral lesions (OL) are a common clinical problem for orthopedic surgeons worldwide and are associated with multiple clinical scenarios ranging from trauma to osteonecrosis. OL vary from chondral lesions in that they involve the subchondral bone and chondral surface, making their management more complex than an isolated chondral injury. Subchondral bone involvement allows for a natural healing response from the body as marrow elements are able to come into contact with the defect site. However, this repair is inadequate resulting in fibrous scar tissue. The second differentiating feature of OL is that damage to the subchondral bone has deleterious effects on the mechanical strength and nutritive capabilities to the chondral joint surface. The clinical solution must, therefore, address both the articular cartilage as well as the subchondral bone beneath it to restore and preserve joint health. Both cartilage and subchondral bone have distinctive functional requirements and therefore their physical and biological characteristics are very much dissimilar, yet they must work together as one unit for ideal joint functioning. In the past, the obvious solution was autologous graft transfer, where an osteochondral bone plug was harvested from a non-weight bearing portion of the joint and implanted into the defect site. Allografts have been utilized similarly to eliminate the donor site morbidity associated with autologous techniques and overall results have been good but both techniques have their drawbacks and limitations. Tissue engineering has thus been an attractive option to create multiphasic scaffolds and implants. Biphasic and triphasic implants have been under explored and have both a chondral and subchondral component with an interface between the two to deliver an implant which is biocompatible and emulates the osteochondral unit as a whole. It has been a challenge to develop such implants and many manufacturing techniques have been utilized to bring together two unalike materials and combine them with cellular therapies. We summarize the functions of the osteochondral unit and describe the currently available management techniques under study.

show abstract

“…Here, the scaffolds made for tissue engineering applications, especially the osteochondral scaffolds, have become very useful. Three types of biomaterial-based scaffolds have been used for this purpose 36 : Monophasic scaffolds, in which one material with homogenous porosity is used. Biphasic scaffolds, in which either two different materials or one material consisting of two parts with different porosities are used.…”

Section: Introductionmentioning

confidence: 99%

“… Biphasic scaffolds, in which either two different materials or one material consisting of two parts with different porosities are used. Triphasic or multiphasic scaffolds, are scaffolds with three or more materials, or one material consisting of three or more parts with different porosities are employed 36 . …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A tri-component knee plug for the 3rd generation of autologous chondrocyte implantation

Tayebi

Cui

2020

Sci Rep

View full text Add to dashboard Cite

Here, we report a newly designed knee plug to be used in the 3rd generation of Autologous Chondrocyte Implantation (ACI) in order to heal the damaged knee cartilage. It is composed of three components: The first component (Bone Portion) is a 3D printed hard scaffold with large pores (~ 850 µm), made by hydroxyapatite and β-tricalcium phosphate to accommodate the bony parts underneath the knee cartilage. It is a cylinder with a diameter of 20 mm and height of 7.5 mm, with a slight dome shape on top. The plug also comprises a Cartilage Portion (component 2) which is a 3D printed gelatin/elastin/sodium-hyaluronate soft thick porous membrane with large pores to accommodate chondrocytes. Cartilage Portion is secured on top of the Bone Portion using mechanical interlocking by designing specific knobs in the 3D printed construct of the Cartilage Portion. The third component of the plug (Film) is a stitchable permeable membrane consisting of polycaprolactone (PCL) on top of the Cartilage Portion to facilitate sliding of the knee joint and to hold the entire plug in place while allowing nutrients delivery to the Cartilage Portion. The PCL Film is prepared using a combination of film casting and sacrificial material leaching with a pore size of 10 µm. It is surface modified to have specific affinity with the Cartilage Portion. The detailed design criteria and production process of this plug is presented in this report. Full in vitro analyses have been performed, which indicate the compatibility of the different components of the plug relative to their expected functions.

show abstract

Recent Approaches to the Manufacturing of Biomimetic Multi-Phasic Scaffolds for Osteochondral Regeneration

Cited by 47 publications

References 55 publications

Initiation and emerging complexity of the collagen network during prenatal skeletal development

Initiation and emerging complexity of the collagen network during prenatal skeletal development

Osteochondral Injury, Management and Tissue Engineering Approaches

A tri-component knee plug for the 3rd generation of autologous chondrocyte implantation

Contact Info

Product

Resources

About