Increased groundwater abstraction is important to the economic development of Africa and to achieving many of the Sustainable Development Goals. However, there is little information on long-term or seasonal groundwater trends due to a lack of in situ monitoring. Here, we used GRACE data from three products (the Centre for Space Research land solution (CSR), the Jet Propulsion Laboratory's Global Mascon solution (JPL-MSCN), and the Centre National D'etudes Spatiales / Groupe de Recherches de Géodésie Spatiale solution (GRGS)), to examine terrestrial water storage (TWS) changes in 12 African sedimentary aquifers, to examine relationships between TWS and rainfall , and estimate groundwater storage (GWS) changes using four Land Surface Models (LSMs) (Community Land Model (CLM2.0), the Variable Infiltration Capacity model (VIC), the Mosaic model (MOSAIC) and the Noah model (NOAH)). We find that there are no substantial continuous long-term decreasing trends in groundwater storage from 2002 to 2016 in any of the African basins, however, consistent rising groundwater trends amounting to~1 km 3 /year and 1.5 km 3 /year are identified in the Iullemmeden and Senegal basins, respectively, and longer term variations in ∆TWS in several basins associated with rainfall patterns. Discrete seasonal ∆TWS responses of ±1-5 cm/year are indicated by GRACE for each of the basins, with the exception of the Congo, North Kalahari, and Senegal basins, which display larger seasonal ∆TWS equivalent to approx. ±11-20 cm/year. The different seasonal responses in ∆TWS provide useful information about groundwater, including the identification of 5 to 9 month accumulation periods of rainfall in many semi-arid and arid basins as well as differences in ∆TWS responses between Sahelian and southern African aquifers to similar rainfall, likely reflecting differences in landcover. Seasonal ∆GWS estimated by combining GRACE ∆TWS with LSM outputs compare inconsistently to available in situ measurements of groundwater recharge from different basins, highlighting the need to further develop the representation of the recharge process in LSMs and the need for more in situ observations from piezometry.