Recent development and application of ratiometric electrochemical biosensor

Ratiometric Electrochemical Sensor for Butralin Determination Using a Quinazoline-Engineered Prussian Blue Analogue

“…Traditional electrochemical sensors usually depend on the precise measurement of a single current intensity, which further leads to low repeatability, reliability, and accuracy, and occasionally false negative results [ 11 ]. For this reason, ratiometric electrochemical sensors have recently attracted extensive attention [ 12 , 13 , 14 , 15 ].…”

Precise Differentiation of Wobble-Type Allele via Ratiometric Design of a Ligase Chain Reaction-Based Electrochemical Biosensor for CYP2C19*2 Genotyping of Clinical Samples

“…Hence, given the similar electrochemical response between methylene blue (MB) and ferrocene (Fc), when the CYP2C19*2 AA homozygote was equally input into these two systems, a higher current response was obtained from MB in ∼−0.25 V than from Fc in ∼0.3 V, so that the current ratio of I MB / I Fc exceeded above 1, which was opposite for the input of the CYP2C19*2 GG homozygote (the current ratio of I MB / I Fc was far below 1), and approximated to 1 with the input of the CYP2C19*2 GA heterozygote. Of note, the ratiometric strategy provided a built-in self-calibrating capability to ameliorate the possible external influence and false signal. , Therefore, the proposed R-eLCR readily achieved a robust and precise allele typing, which provides a reliable alternative for the identification of wobble-type allele and has a promising clinical value for precision medicine.…”

“…Of note, the ratiometric strategy provided a built-in self-calibrating capability to ameliorate the possible external influence and false signal. 28,32 Therefore, the proposed R-eLCR readily achieved a robust and precise allele typing, which provides a reliable alternative for the identification of wobble-type allele and has a promising clinical value for precision medicine. S1.…”

“…However, despite the high accuracy in SNP analysis of genomic DNA in human whole blood, they fail to restrict the the wobble-base pair (GT and AC)-induced mismatch amplification. Meanwhile, all the existing allele typing methods adopt a single-signal output mode, ,− in which the detection results are dependent on the absolute value that is prone to false results and inaccurate readings due to the bias in instrumentation, reagent, environmental conditions, operation, etc. , Therefore, it is essential to explore a novel alternative to circumvent the drawbacks of the available approaches.…”

A colorimetric sensor with dual-ratio and dual-mode for detection of nicotine in tobacco samples

“…With the cross-disciplinary integration, ratiometric sensing methods have also become a hot topic in electrochemical and UV-vis spectroscopy sensing. 21,22 Compared with traditional single-signal detection, ratiometric sensors introduce other active substances as internal reference signals, and use the ratio of dual/multiple signals as output signals, which can achieve high sensitivity, high reliability and real-time calibration of detection results, and effectively avoid errors or false negative signals caused by external environmental interference. 23,24 As portable UV-vis spectrophotometers become more advanced, the application of UV-vis spectrophotometry for real sample analysis becomes more signicant.…”