Recent developments and future directions in the paratransgenesis based control of Leishmania transmission

Wijerathna, Tharaka; Gunathunga, Samadhi U.; Gunathilaka, Nayana

doi:10.1016/j.biocontrol.2020.104260

Cited by 14 publications

(12 citation statements)

References 83 publications

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“…The main requirements for the development of paratransgenesis in sand flies are the same as those in other vector insects (summarised in section Requirements for successful paratransgenesis of present article) , and have been reviewed for phelbotamines by Wijerathna et al [ 309 ]. The prime requirement is the identification of appropriate bacterial species in which to develop the technique.…”

Section: Paratransgenesis In Different Groups Of Vectorsmentioning

confidence: 99%

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Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors

et al. 2022

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This article presents an overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors. It first briefly summarises some of the disease-causing pathogens vectored by insects and emphasises the need for innovative control methods to counter the threat of resistance by both the vector insect to pesticides and the pathogens to therapeutic drugs. Subsequently, the state of art of paratransgenesis is described, which is a particularly ingenious method currently under development in many important vector insects that could provide an additional powerful tool for use in integrated pest control programmes. The requirements and recent advances of the paratransgenesis technique are detailed and an overview is given of the microorganisms selected for genetic modification, the effector molecules to be expressed and the environmental spread of the transgenic bacteria into wild insect populations. The results of experimental models of paratransgenesis developed with triatomines, mosquitoes, sandflies and tsetse flies are analysed. Finally, the regulatory and safety rules to be satisfied for the successful environmental release of the genetically engineered organisms produced in paratransgenesis are considered. Graphical Abstract

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Section: Paratransgenesis In Different Groups Of Vectorsmentioning

confidence: 99%

“…[ 97 , 318 – 328 ]). Fortunately, the extensive reviews of sand flies by Telleria et al [ 315 ], Wijerathna et al [ 309 ] and Omondi and Demir [ 329 ] provide summaries of many of these studies which, taken together, identify numerous species of bacteria.…”

Section: Paratransgenesis In Different Groups Of Vectorsmentioning

confidence: 99%

Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors

et al. 2022

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“…Examples of paratransgenic control approaches come also from the study of leishmaniases and trypanosomiasis. Engineered bacteria of the genus Bacillus, among others, are under study for their potential to reduce the capability of sand flies to transmit Leishmania (Wijerathna et al, 2020). In African trypanosomiasis, the symbiont of the genus Sodalis has been studied as a candidate vector to be exploited to block trypanosome transmission in the tsetse flies.…”

Section: Paratransgenesis For the Control Of Vector-borne Diseasesmentioning

confidence: 99%

Mosquito Trilogy: Microbiota, Immunity and Pathogens, and Their Implications for the Control of Disease Transmission

et al. 2021

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In mosquitoes, the interaction between the gut microbiota, the immune system, and the pathogens that these insects transmit to humans and animals is regarded as a key component toward the development of control strategies, aimed at reducing the burden of severe diseases, such as malaria and dengue fever. Indeed, different microorganisms from the mosquito microbiota have been investigated for their ability to affect important traits of the biology of the host insect, related with its survival, development and reproduction. Furthermore, some microorganisms have been shown to modulate the immune response of mosquito females, significantly shaping their vector competence. Here, we will review current knowledge in this field, focusing on i) the complex interaction between the intestinal microbiota and mosquito females defenses, both in the gut and at humoral level; ii) how knowledge on these issues contributes to the development of novel and targeted strategies for the control of mosquito-borne diseases such as the use of paratransgenesis or taking advantage of the relationship between Wolbachia and mosquito hosts. We conclude by providing a brief overview of available knowledge on microbiota-immune system interplay in major insect vectors.

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“…In the present study, dissected midgut lysates were cultured to isolate the bacterial colonies, before 16S rRNA gene sequencing. The main reason for using a traditional-based screening method rather than direct next-generation sequencing (NGS) was to isolate the bacterial species that can be cultured, which may be beneficial for paratransgenesis application if sufficient species with the required characteristics can be isolated [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Diversity of midgut bacteria in larvae and females of Aedes aegypti and Aedes albopictus from Gampaha District, Sri Lanka

et al. 2021

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Background The midgut microbiota of mosquitoes maintain basal immune activity and immune priming. In recent years, scientists have focused on the use of microbial communities for vector control interventions. In the present study, the midgut bacteria of larvae and adults of Aedes aegypti and Ae. albopictus were assessed using both field-collected and laboratory-reared mosquitoes from Sri Lanka. Methods Adults and larvae of Ae. aegypti and Ae. albopictus were collected from three selected areas in Gampaha Medical Officer of Health area, Gampaha District, Western Province, Sri Lanka. Bacterial colonies isolated from mosquito midgut dissections were identified by PCR amplification and sequencing of partial 16S rRNA gene fragments. Results Adults and larvae of Ae. aegypti and Ae. albopictus harbored 25 bacterial species. Bacillus endophyticus and Pantoea dispersa were found more frequently in field-collected Ae. aegypti and Ae. albopictus adults, respectively. The midgut bacteria of Ae. aegypti and Ae. albopictus adults (X2 = 556.167, df = 72, P < 0.001) and larvae (X2 = 633.11, df = 66, P < 0.001) were significantly different. There was a significant difference among the bacterial communities between field-collected adults (X2 = 48.974, df = 10, P < 0.001) and larvae (X2 = 84.981, df = 10, P < 0.001). Lysinibacillus sphaericus was a common species in adults and larvae of laboratory-reared Ae. aegypti. Only P. dispersa occurred in the field-collected adults of Ae. aegypti and Ae. albopictus. Species belonging to genera Terribacillus, Lysinibacillus, Agromyces and Kocuria were recorded from Aedes mosquitoes, in accordance with previously reported results. Conclusions This study generated a comprehensive database on the culturable bacterial community found in the midgut of field-collected (Ae. aegypti and Ae. albopictus) and laboratory-reared (Ae. aegypti) mosquito larvae and adults from Sri Lanka. Data confirm that the midgut bacterial diversity in the studied mosquitoes varies according to species, developmental stage and strain (field vs laboratory). Graphical abstract

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Recent developments and future directions in the paratransgenesis based control of Leishmania transmission

Cited by 14 publications

References 83 publications

Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors

Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors

Mosquito Trilogy: Microbiota, Immunity and Pathogens, and Their Implications for the Control of Disease Transmission

Diversity of midgut bacteria in larvae and females of Aedes aegypti and Aedes albopictus from Gampaha District, Sri Lanka

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