Recent Developments in Capillary Electrophoresis–Mass Spectrometry of Proteins and Peptides

Monton, Maria Rowena N.; Terabe, Shigeru

doi:10.2116/analsci.21.5

Cited by 62 publications

(5 citation statements)

References 144 publications

(165 reference statements)

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“…The CE separation conditions have a direct impact on the ESI-MS analysis and several parameters have to be considered for CE-ESI-MS analysis [28][29][30][31]. In this study, a number of parameters were initially tested with CE-UV, including coating stability, pH and ionic strength of the separation buffer, injection times and different separation voltages.…”

Section: Resultsmentioning

confidence: 99%

Analysis of peptides using N‐methylpolyvinylpyridium as silica surface modifier for CE‐ESI‐MS

et al. 2010

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In this study, the N-methylpolyvinylpyridinuim polymer has for the first time been used as a silica surface modifier for CE in combination with ESI MS (CE-ESI-MS). The compatibility for ESI-MS was demonstrated by the analysis of peptides and protein digests. The N-methylpolyvinylpyridium surface interacts electrostatically with the ionized silanol groups, giving a cationic surface with a reversed EOF. The surface modifier gave rapid and repeatable separations of peptides, proteins and protein digests at acidic pH for more than 4 h of continuous use. The CE separation yielded peak efficiencies of up to 4.3 x 10(5) plates/m. The surface coating is highly compatible with ESI and facilitates the separation and analysis of complex peptide mixtures as shown by the analysis of BSA digests.

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Section: Resultsmentioning

confidence: 99%

Analysis of peptides using N‐methylpolyvinylpyridium as silica surface modifier for CE‐ESI‐MS

et al. 2010

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“…Recent developments of instrumentation for the hyphenation of capillary electrophoresis to mass spectrometry (CE-MS) have generated significant interest in this technique for the characterization of biomolecules [196,197] such as peptides [198], proteins [199][200][201][202] or biopharmaceuticals [203][204][205]. Different electrophoretic modes such as capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF) or capillary gel electrophoresis (CGE) can be employed to separate entities according to their charge or their size.…”

Section: Source Of Uncertaintymentioning

confidence: 99%

Establishment of measurement traceability for peptide and protein quantification through rigorous purity assessment—a review

Josephs

Martos

et al. 2019

Metrologia

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The health of their populations and efficient health care systems are of critical importance to the economic and social well-being of nations. Accurate and comparable peptide/protein measurements are required in support of diagnosis, prognosis, monitoring and treatment of widespread diseases (e.g. diabetes). The required consistency of measurement results can be achieved by making them traceable to stated references and through the development of Reference Measurement Systems. The review mainly concentrates on the progress made in the Protein Analysis Working Group of the Consultative Committee for Amount of Substance: Metrology in Chemistry and Biology (CCQM-PAWG) in establishing Primary Calibration Reference Services in the emerging area of health markers such as peptides/proteins. Primary Calibration Reference Services are technical capabilities for composition assignment, commonly as the mass fraction content, of pure substances or solutions thereof. It is a core technical competency for National Measurement Institutes (NMIs). A limited number of key comparisons, foreseen by the CCQM-PAWG strategy, are discussed that enable NMIs providing measurement services in peptide/protein analysis to test and demonstrate their capabilities. In addition, the review examines the development and improvement of analytical methods and metrological models that are required to meet the needs of NMIs and associated clinical stakeholders.

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“…Capillary electrophoresis (CE), possibly coupled to MS, can be a good alternative to these chromatography techniques [26,27]. In addition to its various possibilities of coupling detection, CE is a high resolution technique, particularly for enantiomers [28].…”

Section: Introductionmentioning

confidence: 99%

Online large volume sample staking preconcentration and separation of enantiomeric GHRH analogs by capillary electrophoresis

et al. 2023

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A capillary electrophoresis method is proposed to analyze the four most wellknown growth hormone-releasing hormone (GHRH) analogs that are misused by athletes. Dimethyl-β-cyclodextrin used as a chiral selector allowed, for the first time, the separation of those basic peptide analogs, including enantiopeptides (sermorelin and CJC-1293) that differ by the chirality of only one amino acid. To increase the method sensitivity, electrokinetic preconcentration methods have been investigated. The large volume sample stacking with polarity switching (PS-LVSS) method with an injected sample volume corresponding to 80% of the capillary one was found superior to the sweeping in terms of signal enhancement factor (SEF). Acid and organic solvent addition to the sample (0.1 mM phosphoric acid with 30% methanol) led to a twofold signal improvement, when compared to water as a matrix. We increased capillary dimensions to provide a signal enhancement through the injection of a larger sample volume. Finally, using a combination of the optimized PS-LVSS preconcentration with the chiral capillary zone electrophoresis (CZE), the GHRH analogs were separated and limits of detection between 75 and 200 ng/mL were reached. This method was successfully applied to urine after a desalting step. An optimized C18 SPE was used for that purpose in order to provide low sample conductivity (<130 µS/cm) and preserve the efficiency of LVSS preconcentration. SEF of 640 was obtained with desalted urine spiked with sermorelin by comparison to the CZE (without preconcentration) method.

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Recent Developments in Capillary Electrophoresis–Mass Spectrometry of Proteins and Peptides

Cited by 62 publications

References 144 publications

Analysis of peptides using N‐methylpolyvinylpyridium as silica surface modifier for CE‐ESI‐MS

Analysis of peptides using N‐methylpolyvinylpyridium as silica surface modifier for CE‐ESI‐MS

Establishment of measurement traceability for peptide and protein quantification through rigorous purity assessment—a review

Online large volume sample staking preconcentration and separation of enantiomeric GHRH analogs by capillary electrophoresis

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