Recent Developments in Microfluidic<scp>‐Based</scp>Point‐of‐care Testing (<scp>POCT</scp>) Diagnoses

Wang, Dong; Chan, Ho Nam; Liu, Zeyu; Micheal, S.E.; Li, Lijun; Baniani, Dorsa B.; Tan, Ming Jun Andrew; Huang, Lu; Wang, Jiantao; Wu, Hongkai

doi:10.1002/9783527818341.ch8

Cited by 12 publications

(3 citation statements)

References 126 publications

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“…The miniaturization and standardization of the systems for on-site diagnostic purposes will be a future technological challenge. However, the more prevalent use of microfluidic devices will enable greater access to diagnostic testing for patients [ 122 ]. Therefore, there is a need for an efficient system that must be integrated on a chip for on-site sample preparation without any need for extra steps Research in the domain of microfluidic plasma separation will focus on creating such apparatuses that isolate pure and high yield plasma of greater volumes, because assays for the detection of blood biomarkers present in low quantities require large volumes of plasma.…”

Section: Limits Challenges and Policy Recommendationsmentioning

confidence: 99%

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

et al. 2023

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Conventional diagnostic techniques are based on the utilization of analyte sampling, sensing and signaling on separate platforms for detection purposes, which must be integrated to a single step procedure in point of care (POC) testing devices. Due to the expeditious nature of microfluidic platforms, the trend has been shifted toward the implementation of these systems for the detection of analytes in biochemical, clinical and food technology. Microfluidic systems molded with substances such as polymers or glass offer the specific and sensitive detection of infectious and noninfectious diseases by providing innumerable benefits, including less cost, good biological affinity, strong capillary action and simple process of fabrication. In the case of nanosensors for nucleic acid detection, some challenges need to be addressed, such as cellular lysis, isolation and amplification of nucleic acid before its detection. To avoid the utilization of laborious steps for executing these processes, advances have been deployed in this perspective for on-chip sample preparation, amplification and detection by the introduction of an emerging field of modular microfluidics that has multiple advantages over integrated microfluidics. This review emphasizes the significance of microfluidic technology for the nucleic acid detection of infectious and non-infectious diseases. The implementation of isothermal amplification in conjunction with the lateral flow assay greatly increases the binding efficiency of nanoparticles and biomolecules and improves the limit of detection and sensitivity. Most importantly, the deployment of paper-based material made of cellulose reduces the overall cost. Microfluidic technology in nucleic acid testing has been discussed by explicating its applications in different fields. Next-generation diagnostic methods can be improved by using CRISPR/Cas technology in microfluidic systems. This review concludes with the comparison and future prospects of various microfluidic systems, detection methods and plasma separation techniques used in microfluidic devices.

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Section: Limits Challenges and Policy Recommendationsmentioning

confidence: 99%

Prospects of Microfluidic Technology in Nucleic Acid Detection Approaches

et al. 2023

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“…The progress of microfluidic technology makes the driving and control of microfluidics more flexible, accurate, and convenient, promoting the development of point-of-care diagnostics, 1,2 biochemical analysis, 3,4 cell culture, 5 and other related fields. 6 As core and typical components of microfluidic systems, 7 micropumps have received widespread attention because of their advantages of miniaturization and integration.…”

Section: Introductionmentioning

confidence: 99%

Multi-step PDMS curing and a controlled separation method for mass manufacturing of high-performance and user-friendly micro-devices: valved micropumps

Du,

Sun,

2024

Lab Chip

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“…Point-of-care testing (POCT) enables the analysis of patient specimens near or at the site of patient care, thus enabling clinical decision-making without any delay. [1][2][3] In recent decades, POCT has been used for self-monitoring of blood glucose levels in patients with diabetes. It has been developed for rapid and efficient monitoring of other diseases, enabling timely treatment by clinicians.…”

Section: Introductionmentioning

confidence: 99%