Recent developments in molecular sensor designs for inorganic pyrophosphate detection and biological imaging

Anbu, Sellamuthu; Paul, Anup; Stasiuk, Graeme J.; Pombeiro, Armando J. L.

doi:10.1016/j.ccr.2020.213744

Cited by 52 publications

(49 citation statements)

References 134 publications

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“…Sodium PPi (E450) and PPPi (E451) are used widely as relatively safe food additives (sequestrants and water retention agents) and as additives in commercial detergents (water softeners). By contrast, PPi has pivotal roles in many physiological reactions, is a known biomarker for various diseases, including cancer and infectious diseases, and is an essential target in diverse fields, including ecological research ( Anbu et al, 2021 ). Therefore, many Zn 2+ - and Cu 2+ -based fluorescent sensors have been developed for the detection of PPi ( Anbu et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…By contrast, PPi has pivotal roles in many physiological reactions, is a known biomarker for various diseases, including cancer and infectious diseases, and is an essential target in diverse fields, including ecological research ( Anbu et al, 2021 ). Therefore, many Zn 2+ - and Cu 2+ -based fluorescent sensors have been developed for the detection of PPi ( Anbu et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

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Pull-Down Into Active Inclusion Bodies and Their Application in the Detection of (Poly)-Phosphates and Metal-Ions

Hrabárová

Belkova

Köszagová

et al. 2022

Front. Bioeng. Biotechnol.

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Inclusion bodies are typically ignored as they are considered unwanted protein waste generated by prokaryotic host cells during recombinant protein production or harmful protein inclusions in human cell biology. However, these protein particles may have applications for in vivo immobilization in industrial biocatalysis or as cell-tolerable protein materials for the pharmaceuticals industry and clinical development. Thus, there is a need to in vivo “pull-down” (insolubilize) soluble enzymes and proteins into inclusion bodies. Accordingly, in this study, sequences from the short-chain polyphosphatase ygiF were used to design pull-down tags capable of detecting (poly)-phosphates and metal ions. These tags were compared with the entire CHAD domain from Escherichia coli ygiF and SACS2 CHAD from Saccharolobus solfataricus. The results demonstrated that highly soluble green fluorescent protein variants could be pulled down into the inclusion bodies and could have modified sensitivity to metals and di-/tri-inorganic phosphates.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Pull-Down Into Active Inclusion Bodies and Their Application in the Detection of (Poly)-Phosphates and Metal-Ions

Hrabárová

Belkova

Köszagová

et al. 2022

Front. Bioeng. Biotechnol.

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“…Recently, we have also devoted to developing fluorogenic differential sensors for rapid recognition of Cu 2+ and Zn 2+ ions and the consequential detection of S 2and PPi anions [7,8]. Very recently, we have arrayed a range of chemosensors (totally 150) developed over a decade from 2010 to 2019 including metal-based and sequential sensors that selectively detect PPi in both environmental and biological scenarios [56].…”

Section: Introductionmentioning

confidence: 99%

A benzimidazole-based new fluorogenic differential/sequential chemosensor for Cu2+, Zn2+, CN-, P2O74-, DNA, its live-cell imaging and pyrosequencing applications

Anbu

Paul

Surendranath

et al. 2021

Sensors and Actuators B: Chemical

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“…[12] While transition metal complexes such as zinc, [13] copper, [14] europium, [15] and palladium [16] with suitable chromophores significantly sense PPi, metal-free fluororeceptors of different architectures [17] also effectively recognize HPPi/PPi by showing the change in emission in the different ways. Anbu et al, have recently reported an up-to-date review on pyrophosphate sensing [18] where the use of different synthetic scaffolds for designing PPi-sensors are press-worthy. In this context, the use of dipyrromethane in the development of HPPi/PPi receptors is less explored.…”

Section: Introductionmentioning

confidence: 99%

Dipyrromethane‐Based Receptor for Fluorometric Sensing of Hydrogenpyrophosphate

et al. 2021

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A new dipyrromethane-based tetramide 1 has been designed and synthesized. It serves as potential chemosensor for fluorometric detection of hydrogenpyrophosphate (HP 2 O 7 3À ; HPPi) in organic and semi-aqueous medium. In CH 3 CN, the tetramide 1 shows strong excimer formation upon complexation with HPPi and results in a ratiometric change in fluorescence. Other anions did not bring such change. In aqueous CH 3 CN, this is observed although the change is less pronounced. Hydrogen bonding of HPPi in the cleft pulls the pendant naphthalene to form the excimer. The complexation has been established by spectroscopic and theoretical studies. The tetramide 1 selectively senses HPPi in CH 3 CN with binding constant and detection limit of 3.2 × 10 3 M À 1 and 2.08 × 10 À 5 M, respectively.

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Recent developments in molecular sensor designs for inorganic pyrophosphate detection and biological imaging

Cited by 52 publications

References 134 publications

Pull-Down Into Active Inclusion Bodies and Their Application in the Detection of (Poly)-Phosphates and Metal-Ions

Pull-Down Into Active Inclusion Bodies and Their Application in the Detection of (Poly)-Phosphates and Metal-Ions

A benzimidazole-based new fluorogenic differential/sequential chemosensor for Cu2+, Zn2+, CN-, P2O74-, DNA, its live-cell imaging and pyrosequencing applications

Dipyrromethane‐Based Receptor for Fluorometric Sensing of Hydrogenpyrophosphate

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