Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

Jagadevan, Sheeja; Banerjee, Avik; Banerjee, Chiranjib; Guria, Chandan; Tiwari, Rameshwar; Baweja, Mehak; Shukla, Pratyoosh

doi:10.1186/s13068-018-1181-1

Cited by 214 publications

(93 citation statements)

References 181 publications

(195 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further research will focus on improving the activity and oxygen resistance of [FeFe]‐hydrogenase, improving photosynthesis efficiency, electron flow in the fermentative pathway, immobilized microbial culture, and the cost of bioreactors. The development of synthetic biology will make it possible for engineered microalgal cells to produce specific products, including biohydrogen and biofuels . Whether biohydrogen produced by microalgae can become a major source of hydrogen in the future depends not only on the development of synthetic biology and microbial genetic engineering, but also, the cost of other efficient hydrogen production methods, and the use and social acceptance of hydrogen energy …”

Section: Perspectivementioning

confidence: 99%

“…The development of synthetic biology will make it possible for engineered microalgal cells to produce specific products, including biohydrogen and biofuels. [299,300] Whether biohydrogen produced by microalgae can become a major source of hydrogen in the future depends not only on the development of synthetic biology and microbial genetic engineering, but also, the cost of other efficient hydrogen production methods, and the use and social acceptance of hydrogen energy. [53]…”

Section: Perspectivementioning

confidence: 99%

See 1 more Smart Citation

Microalgal Hydrogen Production

et al. 2020

View full text Add to dashboard Cite

Microalgae, as facultative aerobic organisms, convert solar energy to produce biohydrogen under anaerobic condition. Biohydrogen is a kind of ideal clean and renewable energy source with great commercial potential. Many endeavors have been focused on improving the biohydrogen yields by various means. Here, the research history of hydrogen production by microalgae (including cyanobacteria and green algae), the characteristics of nitrogenase and hydrogenase, the mechanisms of hydrogen production, the technological progress, and the application of enzymatic, genetic, and metabolic engineering methods to improve hydrogen production by microalgae are reviewed. In addition, the regulation of anaerobic metabolisms of Chlamydomonas reinhardtii after the disruption of key enzymes functioning in the fermentative pathways is discussed. Finally, the main challenges and obstacles facing the more efficient production and commercialization of hydrogen production from microalgae in the future are proposed.

show abstract

Section: Perspectivementioning

confidence: 99%

Section: Perspectivementioning

confidence: 99%

Microalgal Hydrogen Production

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Through the co-ordinated and balanced expression of genes, both native and those introduced from other organisms, resources within an industrial chassis can be siphoned for the commercial production of high-value commodities. This developing interdisciplinary field has the potential to revolutionize natural product discovery from higher plants, by providing a diverse array of tools, technologies and strategies for exploring the large chemically complex space of plant natural products using unicellular organisms (Moses et al 2017;Jagadevan et al 2018).…”

Section: Synthetic Biology Metabolic Engineering and "Omics" Approachesmentioning

confidence: 99%

“…The metabolic approach or synthetic biology has received the most attention, as the design of a metabolic pathway in a new system opens up possibilities for industrialization of microalgae (Andrianantoandro et al 2006). In synthetic biology, the basic gene manipulation process remains the same, including host selection, gene target, plasmid construction, transformation tools, selection system and DNA editing tools Jagadevan et al 2018).…”

Section: Synthetic Biology Metabolic Engineering and "Omics" Approachesmentioning

confidence: 99%

Advances and challenges in genetic engineering of microalgae

Fajardo

Donato

Carrasco

et al. 2019

Reviews in Aquaculture

View full text Add to dashboard Cite

Knowledge at the molecular level of microalgae has obtained great scientific interest in recent years, because of their potential for biofuel production, as well as other highly valued molecules. Recent advances in the area of the genetic manipulation of microalgae open an entirely new field of possible applications for these organisms as biorefineries. This review represents a compendium that gathers the main milestones that have marked the area of genetic engineering in microalgae during the last decade, from classical techniques for the transformation and expression of transgenes, to the most avant‐garde techniques currently used for gene silencing and DNA edition, as well as a discussion about the future perspectives in this field.

show abstract

“…With such versatility, microalgae appear to be a promising biorenewable resource that has the potential to completely replace fossil resources [11]. Research in microalgae biotechnology has increased dramatically since 2005 and has been a very active field in recent years, especially to produce biomass and biofuels [12,110,111,117,118,136,143].…”

Section: Introductionmentioning

confidence: 99%

Techno-Economic Analysis of Biogas Production from Microalgae through Anaerobic Digestion

Wu¹,

Moreira²,

Zhang³

et al. 2019

Anaerobic Digestion

View full text Add to dashboard Cite

Microalgae are a promising feedstock for bioenergy due to higher productivity, flexible growing conditions, and high lipid/polysaccharide content compared to terrestrial biomass. Microalgae can be converted to biogas through anaerobic digestion (AD). AD is a mature technology with a high energy return on energy invested. Microalgae AD can bypass energy intensive dewatering operations that are associated with liquid fuel production from algae. A techno-economic assessment of the commercial feasibility of algae-based biogas production was conducted using Cyanothece BG0011 biomass as an example. BG0011 is a naturally occurring, saline cyanobacterium isolated from Florida Keys. It fixes atmospheric nitrogen and produces exopolysaccharide (EPS). Maximum cell density and EPS concentration of 2.7 and 2.1 g afdw 1 /L (for total algae biomass concentration of 4.8 g afdw/L) were obtained by air sparging. For an areal cell and EPS productivity of 12.4 and 9.6 g afdw/m2/day, respectively, the biomethane production cost was 14.8 $/MMBtu using covered anaerobic lagoon and high-pressure water scrubbing for biogas purification. Electricity production from biogas costs 13 cents/kwh. If areal productivity was increased by 33% from the same system, by sparging air enriched with 1% CO2, then biomethane cost was reduced to 12.16 $/MMBtu and electricity cost to 11 cents/kwh.

show abstract

Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

Cited by 214 publications

References 181 publications

Microalgal Hydrogen Production

Microalgal Hydrogen Production

Advances and challenges in genetic engineering of microalgae

Techno-Economic Analysis of Biogas Production from Microalgae through Anaerobic Digestion

Contact Info

Product

Resources

About