Recent developments in the determination of acetylcholine and choline

This report describes a method for assay of choline (Ch) and acetylcholine (ACh) levels in rodent brain regions by high-performance liquid chromatography combined with continuous-flow fast atom bombardment mass spectrometry. Following an investigation of various extraction procedures and chromatographic conditions, we found that homogenization in acetonitrile in the presence of magnesium sulphate followed by heptane extraction and then a flow rate programme for chromatographic separation yielded the best results of the protocols tested. Under these conditions, the recoveries of Ch and ACh were 75% and 80%, respectively, with detection limits of 5 pmol for Ch and 2 pmol for ACh. Using this method, Ch and ACh levels in rat and mouse brain regions were similar to those reported by other researchers.

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Use of high-performance liquid chromatography continuous-flow fast atom bombardment mass spectrometry for simultaneous determination of choline and acetylcholine in rodent brain regions

Ishimaru

Ikarashi

Maruyama

1993

Biol. Mass Spectrom.

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Determination of acetylcholine and choline in the femtomole range by means of HPLC, a post-column enzyme reactor, and electrochemical detection

Bueren²,

et al. 1987

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Modulation of cerebral acetylcholine metabolism by the dorsal diencephalic conduction system in the rat

Takashima¹,

Takita

Umino

et al. 1992

Neurochemistry International

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Recent developments in the determination of acetylcholine and choline

Cited by 10 publications

References 15 publications

Use of high-performance liquid chromatography continuous-flow fast atom bombardment mass spectrometry for simultaneous determination of choline and acetylcholine in rodent brain regions

Use of high-performance liquid chromatography continuous-flow fast atom bombardment mass spectrometry for simultaneous determination of choline and acetylcholine in rodent brain regions

Determination of acetylcholine and choline in the femtomole range by means of HPLC, a post-column enzyme reactor, and electrochemical detection

Modulation of cerebral acetylcholine metabolism by the dorsal diencephalic conduction system in the rat

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