2004
DOI: 10.1016/j.tibtech.2004.02.011
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Recent developments in the optimization of thermostable DNA polymerases for efficient applications

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Cited by 114 publications
(74 citation statements)
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“…In this study, we used Taq DNA polymerase, an important enzyme in biotechnology (Dalton, 1999(Dalton, , 2001Pavlov et al, 2004), as a model substrate for examining the effects of chaperones on stability and durability of Taq polymerase, by incorporating chaperones into PCR reaction mixtures.…”
Section: Resultsmentioning
confidence: 99%
“…In this study, we used Taq DNA polymerase, an important enzyme in biotechnology (Dalton, 1999(Dalton, , 2001Pavlov et al, 2004), as a model substrate for examining the effects of chaperones on stability and durability of Taq polymerase, by incorporating chaperones into PCR reaction mixtures.…”
Section: Resultsmentioning
confidence: 99%
“…Gram +ve cocci were cultured on Manitoal salt agar. The bacterial isolates were biochemically identified by the following tests Haemolysin production (Pavlov et al, 2004), Methyl red (MR) (MacFaddin, 1985) and VogesProskauer (VP) (MacFaddin,1980). Also, catalase (Koneman et al, 1992) test was performed to Gram +ve cocci.…”
Section: Bacterial Colony Count In Urine Samples Of Uti and Biochemicmentioning
confidence: 99%
“…The reactions were incubated at 37 C overnight before electrophoresis. Electrophoresis was carried out on a 3% agarose gel and visualized after staining with ethidium bromide [Pavlova et al 2004;Federici et al 2003]. …”
Section: Dna Genotypingmentioning
confidence: 99%