Recent discoveries and applications of Anoxybacillus

Goh, Kian Mau; Kahar, Ummirul Mukminin; Chai, Yen Yen; Chong, Chun Shiong; Chai, Kian Piaw; Ranjani, Velayudhan; Illias, Rosli Md.; Chan, Kok‐Gan

doi:10.1007/s00253-012-4663-2

Cited by 112 publications

(80 citation statements)

References 95 publications

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“…For microorganisms to hydrolyze and metabolize insoluble cellulose, extracellular cellulase must be produced that are either free or cell associated [34,35]. It was noticed that all four strains, namely, NAST-SG1, NAST-SK12, NAST-PD13, and NAST-BR26, produce all three types of cellulase (exoglucanases), i.e., they are present in extracellular, intracellular as well as cell-bound fractions.…”

Section: Extracellular Cell-bound and Intracellular Cellulase Activitymentioning

confidence: 99%

Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

2018

J App Biol Biotech

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The present study was conducted to identify and characterize the thermophilic bacteria isolated from five hot springs, namely, Sinkosh, Singha, Bhurung, Ratopani, and Paudwar located in Myagdi district, Nepal, using phenotypic and genotypic methods. The hot spring has temperature 42-62°C and pH 6.5-6.8. Isolation of thermophiles was done using simple enriched nutrient broth media at 60°C. Selected strains were screened for thermostable enzymes; cellulase, hemicellulase, amylase, protease, gelatinase, and lipase using substrates carboxymethylcellulose, xylan, soluble starch, casein, gelatin, and Tween (20, 40, 60, or 80), respectively. The bacteria were grouped into 16 groups based on morphological and biochemical characteristics. 16S rRNA sequence analysis of 16 isolates and phylogenetic analysis showed a cluster of five distinct taxonomic groups. The groups were identified as genus Anoxybacillus, Aeribacillus, Brevibacillus, Bacillus, and Geobacillus, based on ≥95% similarity with reference strains. This is the first study that reports Anoxybacillus sp., Brevibacillus sp., and Aeribacillus sp. from the hot springs of Nepal.

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Section: Extracellular Cell-bound and Intracellular Cellulase Activitymentioning

confidence: 99%

Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

2018

J App Biol Biotech

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“…Currently, scientists have great interest in studying thermophilic Anoxybacillus, since knowledge accumulated from fundamental and applied studies suggests that this genus can serve as a good alternative in many 420 F. Matpan Bekler & K. Güven environmental, enzyme and biotechnology applications (Goh et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Isolation and production of thermostable α-amylase from thermophilic Anoxybacillus sp. KP1 from Diyadin hot spring in Ağri, Turkey

Bekler

Güven

2014

Biologia

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A novel amylolytic enzyme producing thermophilic bacterial strain KP1 from the Diyadin hot spring water in Agri, Turkey, was isolated in the present study. Phylogenetic analysis based on the partial 16S rRNA gene, biochemical and physiological tests revealed that the strain KP1 belongs to the genus Anoxybacillus. The pH and temperature optima for the α-amylase production by Anoxybacillus sp. KP1 were 8.0 and 50• C, respectively, where the maximum growth was obtained at the 28 th hour of incubation and the highest α-amylase activity was obtained at the 40 th hour of incubation (8979.6 U/mL). The optimum pH and temperature for the enzyme activity were 8.0 and 60• C, respectively. The maximum α-amylase production was secreted in the presence of 2% (w/v) soluble starch (10837.7 U/mL). Among the various organic and inorganic nitrogen sources tested, while keeping the beef extract concentration constant, casamino acid (14310.6 U/mL), urea (14126 U/mL), and tryptone (13217.2 U/mL) at a concentration of 2% gave the maximum α-amylase production. The enzyme activity was enhanced in the presence of 1.5 mM Mn 2+ (123%), whereas it was strongly inhibited 1.5 mM by Hg 2+ . Inhibition by 89% was obtained also with sodium dodecyl sulphate (1%). The enzyme was found to be relatively stable at a range of pH and temperature.

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“…Because these bacteria are alkali-tolerant thermophiles, they are suitable for many industrial applications. More than a decade after the first report of Anoxybacillus, knowledge accumulated from fundamental and applied studies suggests that this genus can serve as a good alternative in many applications related to starch and lignocelluloses biomasses, environmental waste treatment, enzyme technology, and possibly bioenergy production (Goh et al, 2013).…”

Section: Speciesmentioning

confidence: 99%

Study on Degradation of Nylon 6 by thermophilic bacteria Anoxybacillus rupiensis Ir3 (JQ912241)

2016

Int. J. Adv. Res. Biol. Sci.

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Polyamide-6 (also known as nylon-6) is one of the biodegradable-resistant synthetic polymers used in the manufacturing of commodity plastic materials. The environmental effects of the persistence of this material in landfill pose a global problem of disposal system. Knowledge of the microbial pattern of interaction with this plastic will provide the biological resources and scientific basis for the development of sustainable disposal and treatment method. Anoxybacillus rupiensis Ir3 (JQ912241) is a novel thermophilic bacterium, it was isolated from hydrocarbon contaminated soil in Iraq and showed good ability to utilize aromatic compounds, It is represented a new carbazole-degrading bacterium. In an attempt to investigate the ability of this bacterium to degrade nylon6, the strain Ir3 was grown on the nylon6 separately as a sole source of carbon and nitrogen. Results showed that strain Ir3 was able to degrade this compound. Optimum conditions for degradation of nylon6 were investigated. It was found that these conditions are growing this bacterium in chemical define media CDM containing (0.5%) nylon6, and incubated with shaking (180rpm) at 65°C for 7 days, and to confirm the ability to degrade nylon6, analytical experiments HPLC (High Performance Liquid Chromatography) and FTIR (Fourier Transmittance Infrared Spectroscopy) were used. The 6-aminohexanoic acid as intermediate products in the culture medium was mentioned by using HPLC, while biodegradation of the nylon6 was monitored by using FTIR.

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Recent discoveries and applications of Anoxybacillus

Cited by 112 publications

References 95 publications

Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

Isolation and production of thermostable α-amylase from thermophilic Anoxybacillus sp. KP1 from Diyadin hot spring in Ağri, Turkey

Study on Degradation of Nylon 6 by thermophilic bacteria Anoxybacillus rupiensis Ir3 (JQ912241)

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