Recent Insights on Alzheimer’s Disease Originating from Yeast Models

Seynnaeve, David; Vecchio, Mara Del; Fruhmann, Gernot; Verelst, Joke; Cools, Melody; Beckers, Jimmy; Mulvihill, Daniel P.; Winderickx, Joris; Franssens, Vanessa

doi:10.3390/ijms19071947

Cited by 32 publications

(53 citation statements)

References 239 publications

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“…The intriguing fact that most of the processes of ageing are also conserved in yeast makes it an appropriate model for age-related neurodegenerative diseases. S. cerevisiae cells bud asymmetrically for up to 20-25 cycles, indicating a limited replicative lifespan [16]. The division of cells produces smaller daughter cells and leaves scars on the cell surface [391].…”

Section: Future Perspectives Of Using Yeast As a Model Organism For Amentioning

confidence: 99%

“…These intracellular cues are also conserved in yeast cells with more than 60% of its genes having human homologs or at least one conserved domain [15]. The major yeast models used for AD studies involve Saccharomyces cerevisiae [16]. These models for AD studies have been very important in understanding the role of conserved fundamental eukaryotic processes in AD pathology to develop methods to find potential therapeutics [17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

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Protein Homeostasis Networks and the Use of Yeast to Guide Interventions in Alzheimer’s Disease

Dhakal

Macreadie

2020

IJMS

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Alzheimer’s Disease (AD) is a progressive multifactorial age-related neurodegenerative disorder that causes the majority of deaths due to dementia in the elderly. Although various risk factors have been found to be associated with AD progression, the cause of the disease is still unresolved. The loss of proteostasis is one of the major causes of AD: it is evident by aggregation of misfolded proteins, lipid homeostasis disruption, accumulation of autophagic vesicles, and oxidative damage during the disease progression. Different models have been developed to study AD, one of which is a yeast model. Yeasts are simple unicellular eukaryotic cells that have provided great insights into human cell biology. Various yeast models, including unmodified and genetically modified yeasts, have been established for studying AD and have provided significant amount of information on AD pathology and potential interventions. The conservation of various human biological processes, including signal transduction, energy metabolism, protein homeostasis, stress responses, oxidative phosphorylation, vesicle trafficking, apoptosis, endocytosis, and ageing, renders yeast a fascinating, powerful model for AD. In addition, the easy manipulation of the yeast genome and availability of methods to evaluate yeast cells rapidly in high throughput technological platforms strengthen the rationale of using yeast as a model. This review focuses on the description of the proteostasis network in yeast and its comparison with the human proteostasis network. It further elaborates on the AD-associated proteostasis failure and applications of the yeast proteostasis network to understand AD pathology and its potential to guide interventions against AD.

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Section: Future Perspectives Of Using Yeast As a Model Organism For Amentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protein Homeostasis Networks and the Use of Yeast to Guide Interventions in Alzheimer’s Disease

Dhakal

Macreadie

2020

IJMS

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“…Yeast models Yeast, in particular Saccharomyces cerevisiae, is one of the simplest and most-studied eukaryotic organisms available for the study of cellular pathologies (Verduyckt et al, 2016;Tenreiro et al, 2017;Seynnaeve et al, 2018;Rencus-Lazar et al, 2019). This organism has a short generation time, and an arsenal of research tools is available for its study (Smith and Snyder, 2006).…”

Section: Aβ Modelsmentioning

confidence: 99%

Biophysical studies of protein misfolding and aggregation in in vivo models of Alzheimer's and Parkinson's diseases

Sinnige

Stroobants

Dobson

et al. 2020

Quart. Rev. Biophys.

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Neurodegenerative disorders, including Alzheimer's (AD) and Parkinson's diseases (PD), are characterised by the formation of aberrant assemblies of misfolded proteins. The discovery of disease-modifying drugs for these disorders is challenging, in part because we still have a limited understanding of their molecular origins. In this review, we discuss how biophysical approaches can help explain the formation of the aberrant conformational states of proteins whose neurotoxic effects underlie these diseases. We discuss in particular models based on the transgenic expression of amyloid-β (Aβ) and tau in AD, and α-synuclein in PD. Because biophysical methods have enabled an accurate quantification and a detailed understanding of the molecular mechanisms underlying protein misfolding and aggregation in vitro, we expect that the further development of these methods to probe directly the corresponding mechanisms in vivo will open effective routes for diagnostic and therapeutic interventions.

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“…Fission yeast is an excellent system to study the complex intracellular mechanisms underlying neurodegenerative diseases such as Alzheimer's disease (AD). Heterologous expression of Tau and Aβ can provide new insights into the pathobiology of these proteins in vivo as well as the screening of compounds that may be useful in treatment and/or prevention of AD [45]. Recently, it was reported that ginger (dietary condiment) fermented with S. pombe had neuroprotective effects on in vivo models of AD.…”

Section: Heterologous Protein Expressionmentioning

confidence: 99%

Molecular Tools for Gene Analysis in Fission Yeast

Herrera-Camacho¹,

Millán-Pérez-Peña²,

Sosa-Jurado³

et al. 2020

Biochemical Analysis Tools - Methods for Bio-Molecules Studies

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Schizosaccharomyces pombe or fission yeast has been called micromammal due to the potential application of the knowledge derived from the yeast in the physiology of higher eukaryotes. Fission yeast has been consolidated as an excellent model for the study of highly conserved cellular processes. The possibility of using haploid or diploid strains facilitates the analysis of the dominant or recessive phenotype of an allele as well as its function, making it a model of first choice for the development of any investigation in eukaryotes cells. With a growing community that employs fission yeast as a model system for the study of numerous cellular processes, it has motivated the simultaneous development of molecular tools that facilitate the study of genes and proteins in the yeast. In this review, we present the most used molecular techniques in fission yeast for the analysis of genes, its characterization, as well as the determination of its function.

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Recent Insights on Alzheimer’s Disease Originating from Yeast Models

Cited by 32 publications

References 239 publications

Protein Homeostasis Networks and the Use of Yeast to Guide Interventions in Alzheimer’s Disease

Protein Homeostasis Networks and the Use of Yeast to Guide Interventions in Alzheimer’s Disease

Biophysical studies of protein misfolding and aggregation in in vivo models of Alzheimer's and Parkinson's diseases

Molecular Tools for Gene Analysis in Fission Yeast

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