Recent insights on applications of pullulan in tissue engineering

Singh, Ram Sarup; Kaur, Navpreet; Rana, Vikas; Kennedy, John F.

doi:10.1016/j.carbpol.2016.07.118

Cited by 123 publications

(42 citation statements)

References 71 publications

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“…Biomedical applications of pullulan include drug delivery, gene delivery [21,22], tissue engineering [23], vaccination [24,25], medical imaging [26], plasma expander [27], molecular chaperones [28], and film forming [29].…”

Section: Pullulan Is a Linear And Unbranched Exopolysaccharide Consismentioning

confidence: 99%

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

2018

J App Biol Biotech

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Pullulan is a random coil glucan polymer produced by Aureobasidium pullulans. It is one of the commercially promising biopolymers frequently used in pharmaceutical, food and cosmetics industries. The aim of present study was to isolate an efficient fungal strain producing melanin-free pullulan. The fungal strains were isolated from fresh and decaying leaves of plants and screened for pullulan production. Fungal isolate PUPY-06 was the best producer of pullulan (2.12%, w/v). Morphological studies revealed the oval and cylindrical shape of its cells, budding and presence of septate as well as pseudohyphae. Colonies of the isolate on agar plate cultures were beige-to-orange colored with smooth, mucoid, and butyrous texture. Morphological and biochemical characterization established the fungal isolate as Rhodosporidium sp. 18s rRNA sequencing, and phylogenetic analysis revealed the isolate to be Rhodosporidium paludigenum. Fourier-transform infrared spectroscopy and nuclear magnetic resonance of crude pullulan confirmed its structural characteristics. Maximum production of pullulan was achieved after 7 days of cultivation in shake-flask fermentations. Agitation mode of cultivation supported the higher production of pullulan in comparison to the stationary mode. R. paludigenum PUPY-06 was found as a novel pullulan-producing strain. This is a first report on pullulan production by R. paludigenum PUPY-06.

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Section: Pullulan Is a Linear And Unbranched Exopolysaccharide Consismentioning

confidence: 99%

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

2018

J App Biol Biotech

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“…Porous scaffolds with appropriate mechanical strength and biological properties, such as non-toxic, biocompatible, and biodegradable have played vital roles in cell adherence, proliferation, and growth of cells or tissues in bone repair [5][6][7]. The biodegraded scaffold should not elicit any toxic, harmful, damaging, or immunological response to the living tissues or organs [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Silver-Coated Bioactive Nanocomposite Scaffolds Based on Grafted Beta-Glucan/Hydroxyapatite via Freeze-Drying Method: Anti-Microbial and Biocompatibility Evaluation for Bone Tissue Engineering

et al. 2020

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Advancement and development in bone tissue engineering, particularly that of composite scaffolds, are of great importance for bone tissue engineering. We have synthesized polymeric matrix using biopolymer (β-glucan), acrylic acid, and nano-hydroxyapatite through free radical polymerization method. Bioactive nanocomposite scaffolds (BNSs) were fabricated using the freeze-drying method and Ag was coated by the dip-coating method. The scaffolds have been characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction analysis (XRD) to investigate their functional groups, surface morphology, and phase analysis, respectively. The pore size and porosity of all BNS samples were found to be dependent on silver concentration. Mechanical testing of all BNS samples have substantial compressive strength in dry form that is closer to cancellous bone. The samples of BNS showed substantial antibacterial effect against DH5 alpha E. coli. The biological studies conducted using the MC3T3-E1 cell line via neutral red dye assay on the scaffolds have found to be biocompatible and non-cytotoxic. These bioactive scaffolds can bring numerous applications for bone tissue repairs and regenerations.

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“…On the other hand, pullulan, a linear glucosic polysaccharide produced by Aureobasidium pullulans fungus (Cheng, Demirci, & Catchmark, ; Leathers, ) has a high hydroxyl groups structure that provides exclusive bioactive properties (Cheng et al, ; Chi et al, ; Rekha & Sharma, ) in drug delivery, wound healing, and tissue engineering (Rekha & Sharma, ). Nonetheless, in bone tissue engineering applications, pullulan fails to deliver a surface that supports cell adhesion and diffusion for cell proliferation and osteogenesis (Bae et al, ; Singh, Kaur, Rana, & Kennedy, ). Therefore, different techniques such as deposition of hydroxyapatite (HA) on surface, integration of functional groups, or cell recognizable molecules such as gelatin and alginate (Hutson et al, ; Phadke, Shih, & Varghese, ; Singh et al, ) have been tested in order to improve the biological properties of these polymers.…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, in bone tissue engineering applications, pullulan fails to deliver a surface that supports cell adhesion and diffusion for cell proliferation and osteogenesis (Bae et al, ; Singh, Kaur, Rana, & Kennedy, ). Therefore, different techniques such as deposition of hydroxyapatite (HA) on surface, integration of functional groups, or cell recognizable molecules such as gelatin and alginate (Hutson et al, ; Phadke, Shih, & Varghese, ; Singh et al, ) have been tested in order to improve the biological properties of these polymers. Particularly, pullulan has been shown to enhance surface modification properties of alginate, and this could represent a useful tool for tissue engineering applications (Singh et al, ).…”

Section: Introductionmentioning

confidence: 99%

New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials

Popescu

Magyari

Taulescu

et al. 2018

J Tissue Eng Regen Med

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Composites based on sodium alginate, pullulan, and bioactive SiO -CaO-P O glass-ceramics with copper oxide were prepared as capsules. The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy. The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance. The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained. Different crystalline shapes of hydroxyapatite were formed after in vitro and in vivo trials for the glass-ceramic-polymer composites, the in vitro precipitated apatite was found to be nodular, and the in vivo experiment led to needlelike crystallites formation. Histopathological results showed a good biocompatibility with no significant signs of rejection by the host tissue. These assessments performed on the composites indicate that the studied materials can be considered without any doubt suitable candidates for future bone regeneration applications.

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Recent insights on applications of pullulan in tissue engineering

Cited by 123 publications

References 71 publications

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

Synthesis of Silver-Coated Bioactive Nanocomposite Scaffolds Based on Grafted Beta-Glucan/Hydroxyapatite via Freeze-Drying Method: Anti-Microbial and Biocompatibility Evaluation for Bone Tissue Engineering

New alginate-pullulan-bioactive glass composites with copper oxide for bone tissue regeneration trials

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