Recent Progress in Mass Spectrometry‐Based Single‐Cell Metabolic Analysis

Sun, Xuming; Yu, Yi; Qian, Kun; Wang, Jiayi; Huang, Lin

doi:10.1002/smtd.202301317

Cited by 3 publications

(1 citation statement)

References 125 publications

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“…Untargeted metabolomics has better coverage of metabolites, though it possesses limited reproducibility and poor sensitivity for metabolites with low abundance [47]. Targeted metabolomics is typical for metabolite quantification as a consequence of its high sensitivity, high dynamic extent and faithful quantification accuracy [48,49].…”

Section: An Outline Of Metabolomicsmentioning

confidence: 99%

Recent Proteomics, Metabolomics and Lipidomics Approaches in Meat Safety, Processing and Quality Analysis

Sidira,

Smaoui,

Varzakas

2024

Applied Sciences

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With a view to understand and resolve the complexity of the food matrix, omic technologies alone or in combination are extensively employed. In this sense, the newest developments and advances of proteomics, metabolomics and lipidomics with their unique benefits could simplify and help to understand the link between physiological and pathological activities in biology, physiology, pathology and food science and processing. This review aims to briefly introduce the basis of proteomics, metabolomics and lipidomics, then expansively review their impact on the assessment of meat quality and safety. Here, also, we discuss the application of proteomics, metabolomics and lipidomics for the authentication and adulteration of meat and meat derivatives from different sources and provide some perspectives regarding the use of emerging techniques such as rapid mass spectrometry (MS) and non-invasive measurements for the analysis of meat quality and safety. This paper summarizes all significant investigations into these matters and underlines the advances in analytical chemistry technologies and meat science areas. By emphasizing the requirement for additional examinations, this paper attempts a comprehensive knowledge of “foodomics” and the potential to improve its employment in meat science.

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Section: An Outline Of Metabolomicsmentioning

confidence: 99%

Recent Proteomics, Metabolomics and Lipidomics Approaches in Meat Safety, Processing and Quality Analysis

Sidira,

Smaoui,

Varzakas

2024

Applied Sciences

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Single-Cell Mass Spectrometry Studies of Secondary Drug Resistance of Tumor Cells

Zhu,

Zhang,

Zhao

et al. 2024

Anal. Chem.

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Patients with epidermal growth factor receptor mutant nonsmall cell lung cancer (NSCLC) often fail to treat gefitinib because of secondary drug resistance. The development of tumor drug resistance is closely related to variations in cancer cell metabolism. Single-cell metabolomics analysis can provide unique information about tumor drug resistance. Herein, we constructed a platform to study the secondary resistance of tumor cells based on single-cell metabolomics (sSRTC-scM). A gefitinib-resistant NSCLC cell line (PC9GR) was constructed by increasing the dose step by step. The metabolic profiles of parental PC9 cells and PC9GR cells with different drug resistance levels were detected by intact living-cell electrolaunching ionization mass spectrometry at the single-cell level. The data were analyzed by statistical methods such as t-SNE, variance, volcano plot, heat map, and metabolic pathway analysis. Using this platform, we found that the metabolic fingerprints of PC9GR cells can evaluate drug resistance degrees. The metabolic fingerprints continue to be altered with the increase of drug resistance. We revealed 19 metabolic markers of secondary resistance by variance analysis and clarified that the glycerophospholipid metabolic pathway of PC9GR cells changed significantly. In addition, we found that with the increase in drug resistance levels, the heterogeneity of single-cell metabolism became greater and the number of cells with weak drug resistance gradually decreased. This phenomenon can be utilized to illustrate the drug resistance degrees of PC9GR cells. This study provides diagnostic markers for evaluating the drug resistance of tumors and gives new insight into overcoming the secondary resistance of tumors.

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Injectable Double-Network Hydrogel as Soft Bioelectronics for Epileptic Discharge Monitoring Via Engineered Two-Dimensional-Materials

Zhang,

Rana,

Lin

et al. 2024

Preprint

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Single-component hydrogels often grapple with the formidable challenge of meeting multifaceted capability requirements essential in practical applications, including conductivity, adhesiveness, injectability, and resistance to stretching and bending. In response, we harness a double-network hydrogel (DNH) strategy, augmenting it with engineered two-dimensional-material transition metal boride (MBene) as an enhancer. This innovative strategy enables the creation of MB-DNH hydrogel, showcasing favourable injectability, conductivity, robust adhesion to brain tissue, and resilience against bending and stretching. Consequently, it empowers us to analyze and monitor epileptic abnormal discharges. Regarding conductivity, MB-DNH (0.24 ± 0.009 mS/cm) outperforms two single-network hydrogels (PEDOT: PSS and polyacrylamide), exhibiting enhancements of 0.84 and 25.6 folds, respectively. Regarding adhesiveness, MB-DNH excels, showing increments of 128.8% and 117.7%, respectively, compared to two single-network hydrogels. For mechanical capability, MB-DNH demonstrates favourable resistance to stretching and bending with relative standard deviation (RSD) values of 1.03% and 1.35%, respectively, following 50 stretching and 30 bending cycles. In practical applications, MB-DNH enables electroencephalogram (EEG) recording and monitoring of abnormal discharges in epileptic mice. We envision that this double-network hydrogel strategy, anchored by MBene, will substantially advance precise and efficient EEG recording, propelling progress in brain-machine interfaces and human-computer interaction.

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Recent Progress in Mass Spectrometry‐Based Single‐Cell Metabolic Analysis

Cited by 3 publications

References 125 publications

Recent Proteomics, Metabolomics and Lipidomics Approaches in Meat Safety, Processing and Quality Analysis

Recent Proteomics, Metabolomics and Lipidomics Approaches in Meat Safety, Processing and Quality Analysis

Single-Cell Mass Spectrometry Studies of Secondary Drug Resistance of Tumor Cells

Injectable Double-Network Hydrogel as Soft Bioelectronics for Epileptic Discharge Monitoring Via Engineered Two-Dimensional-Materials

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