Recent progress in small molecule fluorescent probes for nitroreductase

Qin, Wenjing; Xu, Chenchen; Zhao, Yanfei; Yu, Changmin; Shen, Sheng; Li, Lin; Huang, Wei

doi:10.1016/j.cclet.2018.04.007

Cited by 91 publications

(41 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2f and h. Dicoumarin was a well-known inhibitor for NTR. [4,48] Then we incubated cells with 100 μM dicoumarin to inhibit NTR for 2 h. As shown in Fig. 2b, cells showed extremely weak fluorescence signals after incubated with dicoumarin, which further confirmed the selectivity of TP-NO 2 for NTR.…”

Section: Ntr Detection In Hypoxia Cellsmentioning

confidence: 56%

“…Hypoxia can lead to structures, functions and diffusion conditions of tumors disturbed, and it plays significant roles in tumor invasion, metastasis and drug resistance [3]. Researchers report that hypoxia causes many tumor-associated genes overexpress, especially hypoxia inducible factors 1α (HIF-1α) and HIF-mediated genes [4,5,48]. Within the hypoxic tumor microenvironment, HIF-1α promotes angiogenesis, metabolic adaptation, and other key aspects of tumor progression, which results in deteriorating tumor hypoxic microenvironment and abnormal microvessels.…”

Section: Introductionmentioning

confidence: 99%

“…NTR is an endogenous enzyme and closely related to oxygen concentration. The results of cell experiments indicated that the hypoxia degree of tumor cells can be evaluated by NTR detection[4,30,48]. Since our probe TP-NO 2 had been used for NTR detection successfully under different O 2 concentrations, we attempted to evaluate the NTR concentration fluctuations during the process of tumor treatment.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo

Wang

Zhang

Huang

et al. 2020

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Tumor resistance is a huge challenge for tumor treatment, which may lead to tumor treatment failure. Relieving tumor resistance and improving efficacy are long-term goals for tumor treatment. Nitroreductase (NTR) is an endogenous enzyme that highly expressed in hypoxia tumors. Herein, we develop a NTR-activatable fluorescent probe, TP-NO 2 , for NTR detection during tumor treatment. TP-NO 2 with simple synthesis steps and high yield can qualitatively and quantitatively detect NTR in a wide range (0−20 μg/mL) with a detection limit of 26 ng/ mL. The probe has been successfully applied for NTR detection in cells under simulated hypoxia conditions. Hyperbaric oxygen (HBO) can alleviate tumor hypoxia and reduce NTR concentration. We pre-evaluate the effect of tumor treatment by NTR imaging. And the results demonstrate that HBO-assistant chemotherapy can effectively treat tumor cells. We further apply TP-NO 2 for NTR detection in A549 and cis-dichlorodiamineplatinum(II) (DDP)-resistant A549 (A549/DDP) xenograft nude mice, and we choose NTR as an indicator to pre-evaluate the treatment efficacy of malignant tumors. With the adjuvant therapy of HBO, chemotherapeutic drugs gemcitabine and carboplatin can effectively treat A549 and A549/DDP xenograft. These applications provide us a novel perspective for NTR imaging.

show abstract

Section: Ntr Detection In Hypoxia Cellsmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo

Wang

Zhang

Huang

et al. 2020

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

show abstract

“…Therefore, the majority of these fluorogenic nitroaryl derivatives have been successfully used for hypoxia imaging in tumor cells through an effective fluorescence "OFF-ON" mode. [9][10][11][12][13][14] However, just a few of them used a 4,4-difluoro-4-bora-3a,4a-diazas-indacene (Bodipy) dye as fluorescent platform and furthermore, little attention has been paid to the optimization of both spectral features and physicochemical properties (e.g., far-red emission maximum, water solubility, cell permeability) of the corresponding probes.…”

Section: Main Textmentioning

confidence: 99%

Novel Water-Soluble Far-Red Nitroreductase-Responsive Bodipy-Based Fluorescent Probe for the Detection of Hypoxic Status in A549 Non-Small Cell Lung Cancer Cells

GORBATOV¹,

UVAROV²,

Scherbakov³

et al. 2020

Preprint

View full text Add to dashboard Cite

Over expression of nitroreductase (NTR) enzymes is closely related to the hypoxic status in living organisms for which molecular oxygen is vital. The development of effective methods for real-time monitoring of NTR activity is of great significance for medical diagnosis and cancer research. Here, we present a novel water-soluble Bodipy-based chemodosimeter (NBB) effective in imaging the hypoxic status of human non-small-cell lung cancer A549 cells. We assumed that NTR-mediated activation of the probe NBB is based on the reductive release of meso-phenol Bodipy dye to finally produce unusual fluorescence "ON-OFF" response.

show abstract

“…[9,10] In the present of fluorescent probes, NADH also can help nitroreductases to detect and image the hypoxic status of cancer cells. [11] Therefore, as an important co-enzymes and potential biomarker of cancers, it is worthy to devote extensive research attention in the field of NADH detection.…”

Section: Introductionmentioning

confidence: 99%

Indirect Electrochemical Detection of NADH Through an Active Stainless Steel Fiber Felt (SSFF) Electrode Decorated With the Amino‐Graphene/Nafion Nano Composite Films

Liu

et al. 2018

ChemistrySelect

View full text Add to dashboard Cite

As the energy transfer mediator in the metabolism of human beings, dihydronicotinamide adenine dinucleotide (NADH) is considered as an important biomarker, the concentration of which is correlated to several lethal diseases, such as breast cancer and Parkinson's disease. Due to the high oxidation potential encountered for NADH direct oxidation, herein, we report an active electrode for indirect electrochemical detection of NADH at low potential with the inexpensive stainless steel fiber felt (SSFF). The SSFF electrodes were modified through the layer‐by‐layer assembling of amino‐graphene (AG) fragments and Nafion, and characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), scanning electron microscope (SEM) and X‐ray photoelectron spectroscopy (XPS). Taking the peak current at the apparent oxidation potential (‐0.2 V) as the sensing signal, the proposed SSFF active electrode presented a stable logarithmic detection range from 100 nM to 300 μM with R2=0.996. The regeneration process was also explored and proved to be robust. Finally, the SSFF active electrode was applied to sense NADH in a bio‐mixture solution prepared from tumor cell lysate. The experimental results demonstrated that, with a facile processing, the corrosion resistant and low cost SSFF electrodes could serve as a reliable and practical probe for NADH detection, which would further benefit the clinical diagnostics of NADH‐related diseases.

show abstract

Recent progress in small molecule fluorescent probes for nitroreductase

Cited by 91 publications

References 54 publications

Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo

Rational design of a nitroreductase-activatable two-photon fluorescent probe for hypoxia imaging in cell and in vivo

Novel Water-Soluble Far-Red Nitroreductase-Responsive Bodipy-Based Fluorescent Probe for the Detection of Hypoxic Status in A549 Non-Small Cell Lung Cancer Cells

Indirect Electrochemical Detection of NADH Through an Active Stainless Steel Fiber Felt (SSFF) Electrode Decorated With the Amino‐Graphene/Nafion Nano Composite Films

Contact Info

Product

Resources

About