Recent progress in the development of derivatization reagents having a benzofurazan structure

Abstract

Chemical derivatization is often used to enhance the detectability of the target compounds and to improve the separation efficiency in high-performance liquid chromatography (HPLC). In this review, we describe the recent progress in the development of derivatization reagents having a benzofurazan structure, namely, the fluorogenic reagents, water-soluble reagents, reagents for the analysis of peptides and proteins, and reagents for mass spectrometric detection. The application of these reagents to bio-samples is also briefly described.

Determination of L-tryptophan and L-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS

“…To date, several reports have described that the benzofurazan moiety mentioned above is useful not only for fluorescence, but also for MS detection 19,20. Therefore, here, we changed the detection system from a fluorescence to MS system, using a JMS-T100 LP AccuTOF LC-Plus equipped with an ESI source (JEOL Co. Ltd., Tokyo, Japan), as an alternative detection method.…”

New thinking in the development of novel derivatization reagents for liquid chromatography–mass spectrometric detection

“…To the EditorSome recent articles published in the literature provide a great impetus for the continued outside-the-box thinking in the development and applicability of novel reagents for HPLC-and LC/MS-related applications (Santa et al, , 2008aSeiwert and Karst, 2007;Al-Dirbashi et al, 2007;Honda et al, 2007;Timperio et al, 2007;Hammad et al, 2007;Farid et al, 2007;Gao et al, 2005;Halket et al, 2005;Johnson, 2005;Higashi and Shimada, 2004).…”

“…As eloquently captured by a recent work on benzofuran based derivatization reagents, Santa et al (2008a) have enumerated several different options for bioanalytical specialists to exploit, including LC/MS/MS options via ESI. The detailed requirements for LC/ESI-MS/MS derivatization reagents to be excellent have been summarized (Santa et al, , 2008a.…”

“…The detailed requirements for LC/ESI-MS/MS derivatization reagents to be excellent have been summarized (Santa et al, , 2008a. However, it needs to be appreciated that potential for ionization and ease of fragmentation of xenobiotics may not go hand in hand and therefore the need for derivatization becomes of paramount importance to circumvent such difficult analytical dilemmas.…”

Synthesis of benzofurazan derivatization reagents for short chain carboxylic acids in liquid chromatography/electrospray ionization‐tandem mass spectrometry (LC/ESI‐MS/MS)

“…We investigated the relationship between the chemical structure and fragment patterns of benzofurazan compounds and found that benzofurazan compounds having an (N,N-dimethylamino)ethylaminosulfonyl group [-SO 2 NHCH 2 CH 2 N(CH 3 ) 2 ] and an amino group were easily cleaved by CID and generated a single and intense production at m/z 151 in MS/MS (Santa et al, 2007b;2008a). Based on these observations, we have synthesized benzofurazan derivatization reagents suitable for LC/ESI-MS/MS, i.e.…”