Recent progress in tissue optical clearing for spectroscopic application

Sdobnov, Anton; Darvin, Maxim E.; Genina, Elina A.; Bashkatov, Alexey N.; Lademann, Juergen; Tuchin, Valery V.

doi:10.1016/j.saa.2018.01.085

Cited by 97 publications

(69 citation statements)

References 188 publications

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“…Much has been done with this technique in the meantime . The OC technique has proven itself powerful in the visible to near infrared (NIR) range of spectrum . The question now is: “How powerful is the OC technique in the ultraviolet range?” If it is also powerful in the ultraviolet (UV) range, it opens a fundamentally new field of OC which is the only way to advance into the depth of tissues to provide in vivo and in situ study of molecular structures using UV Raman spectroscopy , optical UV histology , quantification of DNA/protein ratio and UV DNA‐protein a zero length cross‐linking .…”

Section: Introductionmentioning

confidence: 99%

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Moving tissue spectral window to the deep‐ultraviolet via optical clearing

Carneiro

Carvalho

Henrique

et al. 2019

Journal of Biophotonics

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The optical immersion clearing technique has been successfully applied through the last 30 years in the visible to near infrared spectral range, and has proven to be a promising method to promote the application of optical technologies in clinical practice. To investigate its potential in the ultraviolet range, collimated transmittance spectra from 200 to 1000 nm were measured from colorectal muscle samples under treatment with glycerol‐water solutions. The treatments created two new optical windows with transmittance efficiency peaks at 230 and 300 nm, with magnitude increasing with glycerol concentration in the treating solution. Such discovery opens the opportunity to develop clinical procedures to perform diagnosis or treatments in the ultraviolet.

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Section: Introductionmentioning

confidence: 99%

“…Much has been done with this technique in the meantime [20,21]. The OC technique has proven itself powerful in the visible to near infrared (NIR) range of spectrum [22][23][24]. The question now is: "How powerful is the OC technique in the ultraviolet range?"…”

Section: Introductionmentioning

confidence: 99%

Moving tissue spectral window to the deep‐ultraviolet via optical clearing

Carneiro

Carvalho

Henrique

et al. 2019

Journal of Biophotonics

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“…Being a potential method to reduce light scattering in biological tissues, the OC technique creates a temporary transparency effect by partial replacement of the interstitial water by an agent with higher RI, better matched to the RI of tissue scatterers . Consequently, higher tissue depths can be reached with considerable beam collimation, allowing image acquisition from deeper tissue layers for a diagnosis or deeper laser surgery .…”

Section: Introductionmentioning

confidence: 99%

A robust ex vivo method to evaluate the diffusion properties of agents in biological tissues

Carneiro

Carvalho

Henrique

et al. 2019

Journal of Biophotonics

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A robust method is presented for evaluating the diffusion properties of chemicals in ex vivo biological tissues. Using this method that relies only on thickness and collimated transmittance measurements, the diffusion properties of glycerol, fructose, polypropylene glycol and water in muscle tissues were evaluated. Amongst other results, the diffusion coefficient of glycerol in colorectal muscle was estimated with a value of 3.3 × 10−7 cm2/s. Due to the robustness and simplicity of the method, it can be used in other fields of biomedical engineering, namely in organ cryoprotection and food industry.

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“…In this way, using the proposed equation based on the diffuse reflectance imaging method can be a potentially additional useful tool for advanced diagnostics of cancerous skin tissues. Also, additional application of optical clearing [28][29][30][31][32][33] can allow enhancing the penetration depth for LSCI as well as quality of the obtained data.…”

Section: Resultsmentioning

confidence: 99%

Combined multi-wavelength laser speckle contrast imaging and diffuse reflectance imaging for skin perfusion assessment

Sdobnov

Bykov

Попов

et al. 2019

Novel Biophotonics Techniques and Applications V

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Laser Speckle Contrast Imaging (LSCI) is a powerful low-cost method for visualization of flow, microcirculation and blood perfusion. Due to the fact that diseased and healthy tissues has different blood perfusion, LSCI can be a perspective tool for cancer diagnostics and discrimination between different types of tissues. Previously, multispectral diffuse reflectance imaging method for melanoma diagnostics has been introduced. In this work, multi-wavelength (532-, 655-and 850-nm) LSCI technique combined with hyperspectral camera and diffuse reflectance imaging method will be used for assessment of tissues with different skin perfusion properties. An in vivo experiment with occlusion in human finger was performed serving as a model of tissues with different perfusion properties. The proposed method still requires further development and improvements to become a real clinical laboratory tool for non-invasive skin cancer diagnostics.

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Recent progress in tissue optical clearing for spectroscopic application

Cited by 97 publications

References 188 publications

Moving tissue spectral window to the deep‐ultraviolet via optical clearing

Moving tissue spectral window to the deep‐ultraviolet via optical clearing

A robust ex vivo method to evaluate the diffusion properties of agents in biological tissues

Combined multi-wavelength laser speckle contrast imaging and diffuse reflectance imaging for skin perfusion assessment

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