Recent progress of ICP-MS in the development of metal-based drugs and diagnostic agents

Timerbaev, Andrei R.

doi:10.1039/c3ja50394a

Cited by 40 publications

(18 citation statements)

References 111 publications

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“…Its usefulness promoted the method's recent arrival in the nano–bio area where CE offers a unique tool to characterize the identity of metal‐containing nanoscale materials upon interaction with biomolecules such as proteins . Identification of individual metal‐based nanoparticles and their protein conjugates, as well as monitoring the speciation alterations in biological systems, is greatly facilitated by online combination of CE with ICP‐MS . In our recent research , a highly sensitive CE‐ICP‐MS method has been developed to assay the speciation of gold nanoparticles (AuNPs) in human serum and hence to evaluate possible changes in speciation on the way from the point of administration to the cell.…”

Section: Stoichiometry Of Protein Interactions With Aunpsmentioning

confidence: 99%

Characterization of the protein corona of gold nanoparticles by an advanced treatment of CE‐ICP‐MS data

et al. 2016

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CE is well known not only as an efficient separation method, but also as a viable tool for studying chemical reactions, including kinetic assaying and analysis of chemical equilibria. In this communication, the latter feature of CE interfaced with ICP-MS was exploited to determine the stoichiometric composition of the protein corona of gold nanoparticles (AuNPs) at equilibrium conditions. For both individual albumin and human serum involved in binding, the number of protein molecules bound per AuNP (n) was calculated. Since the time scale of the corona formation was previously found to be dependent on the particle size, two calculation algorithms were adopted here. In the case of 5-nm AuNPs, rather slowly associating with the protein, the peak areas measured for the conjugated and free particles were taken in computation (the (34) S signal due to bound protein was also monitored simultaneously to confirm that equilibrium is reached). In binding labile systems (10-50 nm AuNPs), the particles are converted into the protein-bound form relatively fast due mostly to the favor of a much greater excess of the protein so that no peak of the free particles interacting with serum being recorded. Therefore, the n value was estimated by relating the sulfur peak area of each of these conjugates to that of 5-nm AuNPs to calculate the number of bound albumin molecules that was then divided by the number of AuNPs. The AuNPs were found to react with from 13 to 292 albumin molecules that is in good agreement with the literature data.

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Section: Stoichiometry Of Protein Interactions With Aunpsmentioning

confidence: 99%

Characterization of the protein corona of gold nanoparticles by an advanced treatment of CE‐ICP‐MS data

et al. 2016

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“…Current optimization of lead compounds is accompanied by solution based experiments mimicking intracellular chemistry. Ex vivo studies using drug incubation in serum 4 and cell lysates 5,6 provided valuable information preceding in vivo and in vitro screening. Combined with mass spectrometric analysis (elemental and molecular mass spectrometry), these types of experiments emerged as a valuable tool supporting preclinical drug development and promoting the basic understanding of drug biomolecule interaction.…”

Section: Introductionmentioning

confidence: 99%

“…Powerful methods for the quantication of metallodrug-protein association and biotransformation were developed for different matrices such as human plasma, serum and cell lysates. 4,5,10 Recently, a systematic study evaluated different methods for quantitative screening of protein adduct formation. These methods included separation methods/ fractionation methods such as centrifugal ultraltration, size exclusion and turbulent ow chromatography in combination with ICP-MS. 11 However, using these methods precluded accurately differentiating between the free intact drug, smallmolecule transformation products and protein adducts.…”

Section: Introductionmentioning

confidence: 99%

Heart-cut 2DSEC-RP-LC-ICP-MS as a screening tool in metal-based anticancer research

Galvez

Rusz

Jakupec

et al. 2019

J. Anal. At. Spectrom.

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“…As an alternative to on-line separation, several studies resorted to an off-line fractionation technique, which had been developed in the late 1960s [26], namely centrifugal ultrafiltration using cut-off filters. As a key advantage, this strategy is technically very simple; however, it became very clear soon that filter material selection and preconditioning strategy was crucial regarding drug recovery [27][28][29][30], making this technique more tedious than expected. Flow injection (FI)-ICP-MS [28,31] proved to be a valuable method addressing the metal-based drug distribution between low (LMF) and high molar mass fraction (HMF) in very small sample volumes.…”

Section: Introductionmentioning

confidence: 99%

“…As a key advantage, this strategy is technically very simple; however, it became very clear soon that filter material selection and preconditioning strategy was crucial regarding drug recovery [27][28][29][30], making this technique more tedious than expected. Flow injection (FI)-ICP-MS [28,31] proved to be a valuable method addressing the metal-based drug distribution between low (LMF) and high molar mass fraction (HMF) in very small sample volumes. In some cases, off-line protein removal was followed by LC-ICP-MS analysis in order to assess potential low molar mass transformation products of the metal-based drugs [16,27,32].…”

Section: Introductionmentioning

confidence: 99%

Critical assessment of different methods for quantitative measurement of metallodrug-protein associations

et al. 2018

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Quantitative screening for potential drug–protein binding is an essential step in developing novel metal-based anticancer drugs. ICP–MS approaches are at the core of this task; however, many applications lack in the capability of large-scale high-throughput screenings and proper validation. In this work, we critically discuss the analytical figures of merit and the potential method-based quantitative differences applying four different ICP–MS strategies to ex vivo drug–serum incubations. Two candidate drugs, more specifically, two Pt(IV) complexes with known differences of binding affinity towards serum proteins were selected. The study integrated centrifugal ultrafiltration followed by flow injection analysis, turbulent flow chromatography (TFC), and size exclusion chromatography (SEC), all combined with inductively coupled plasma-mass spectrometry (ICP–MS). As a novelty, for the first time, UHPLC SEC-ICP–MS was implemented to enable rapid protein separation to be performed within a few minutes at > 90% column recovery for protein adducts and small molecules. Graphical abstractQuantitative screening for potential drug–protein binding is an essential step in developingnovel metal-based anticancer drugs Electronic supplementary materialThe online version of this article (10.1007/s00216-018-1328-8) contains supplementary material, which is available to authorized users.

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Recent progress of ICP-MS in the development of metal-based drugs and diagnostic agents

Abstract: Critical analysis of current capabilities, limitations, and trends of ICP-MS applied to the development of metal-based medicines is conducted.

Cited by 40 publications

References 111 publications

Characterization of the protein corona of gold nanoparticles by an advanced treatment of CE‐ICP‐MS data

Characterization of the protein corona of gold nanoparticles by an advanced treatment of CE‐ICP‐MS data

Heart-cut 2DSEC-RP-LC-ICP-MS as a screening tool in metal-based anticancer research

Critical assessment of different methods for quantitative measurement of metallodrug-protein associations

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