Recent progress on microfluidic biosensors for rapid detection of pathogenic bacteria

Xing, Gaowa; Zhang, Weifei; Li, Nan; Pu, Qiaosheng; Lin, Jin‐Ming

doi:10.1016/j.cclet.2021.08.073

Cited by 97 publications

(38 citation statements)

References 83 publications

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“…The rapid and sensitive detection of pathogenic bacteria viruses is crucial for public health, medical diagnostics, environmental monitoring, and food safety fields in order to avoid outbreaks. Conventional detection methods relying on culturing and biochemical analysis are time consuming and take 2 or 3 days or longer [46]. For instance, it is clear that virus cultivation is tedious work, which requires well-trained personnel and specific methodologies [47].…”

Section: Detection Of Microbial Analytesmentioning

confidence: 99%

Aptamer-Based Lateral Flow Assays: Current Trends in Clinical Diagnostic Rapid Tests

2022

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The lateral flow assay (LFA) is an extensively used paper-based platform for the rapid and on-site detection of different analytes. The method is user-friendly with no need for sophisticated operation and only includes adding sample. Generally, antibodies are employed as the biorecognition elements in the LFA. However, antibodies possess several disadvantages including poor stability, high batch-to-batch variation, long development time, high price and need for ethical approval and cold chain. Because of these limitations, aptamers screened by an in vitro process can be a good alternative to antibodies as biorecognition molecules in the LFA. In recent years, aptamer-based LFAs have been investigated for the detection of different analytes in point-of-care diagnostics. In this review, we summarize the applications of aptamer technology in LFAs in clinical diagnostic rapid tests for the detection of biomarkers, microbial analytes, hormones and antibiotics. Performance, advantages and drawbacks of the developed assays are also discussed.

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Section: Detection Of Microbial Analytesmentioning

confidence: 99%

Aptamer-Based Lateral Flow Assays: Current Trends in Clinical Diagnostic Rapid Tests

2022

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“…Timely and accurate analysis of pathogens in food is of great significance to control food quality and ensure food safety. At present, analytical methods for the detection of foodborne pathogens have been extensively reported, such as nucleic acid‐based detection methods (Zhan et al., 2020), lateral flow assay (K. Luo et al., 2020), microfluidic chip (Xing et al., 2021), and biosensor (F. Huang, Zhang, et al., 2021). Although these techniques have made great contributions to the screening of foodborne pathogens, there are still problems of matrix interference and low sensitivity.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

“…Microfluidic chip is an integrated small‐scale analytical device, known as a laboratory on a chip, which integrates the steps of sample addition, mixing, incubation, washing, separation, and detection and has the advantages of miniaturization, automation, and low cost (Xing et al., 2021). The tortuous channels or grooves designed on the chip can increase the binding chance of MNPs and signal tags with the target pathogens, and the interfering substances are removed by washing under an external magnetic field (Liu et al., 2021; Qi, Zheng, Wang, et al., 2021).…”

Section: Application Of Mnps In Integrated Detection Of Foodborne Pat...mentioning

confidence: 99%

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Xiao

2022

Comp Rev Food Sci Food Safe

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Foodborne pathogens contamination is one of the main sources of food safety problems. Although the existing detection methods have been developed for a long time, the complexity of food samples is still the main factor affecting the detection time and sensitivity, and the rapid separation and enrichment of pathogens is still an objective to be studied. Magnetic separation strategy based on magnetic nanoparticles (MNPs) is considered to be an effective tool for rapid separation and enrichment of foodborne pathogens in food. Therefore, this study comprehensively reviews the development of MNPs in the separation of foodborne pathogens over the past decade. First, various biorecognition reagents for identification of foodborne pathogens and their modifications on the surface of MNPs are introduced. Then, the factors affecting the separation of foodborne pathogens, including the size of MNPs, modification methods, separation strategies and separation forms are discussed. Finally, the application of MNPs in integrated detection methods is reviewed. Moreover, current challenges and prospects of MNPs for the analysis of foodborne pathogens are discussed. Further research should focus on the design of multifunctional MNPs, the processing of large‐scale samples, the simultaneous analysis of multiple targets, and the development of all‐in‐one small analytical device with separation and detection.

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“…Microfluidics refers to the science and technology of precisely controlling and manipulating micro and nanofluids with high precision and reproducibility in the micro and nanoscale space [ 173 , 174 ]. Microfluidic chips, also known as lab-on-a-chip (LOC), can integrate the basic functional units involved in biological and chemical experiments, such as mixing, reaction, separation and detection, on a single chip with a high degree of integration [ 175 ]. In addition, the microfluidic chip is a critical part of the biosensor for microfluidic control technology, and its structure is shown in Figure 5 .…”

Section: Commonly Used Signal Amplification Strategies For Electroche...mentioning

confidence: 99%

Electrochemical Signal Amplification Strategies and Their Use in Olfactory and Taste Evaluation

Wang

Liu

et al. 2022

Biosensors

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Biosensors are powerful analytical tools used to identify and detect target molecules. Electrochemical biosensors, which combine biosensing with electrochemical analysis techniques, are efficient analytical instruments that translate concentration signals into electrical signals, enabling the quantitative and qualitative analysis of target molecules. Electrochemical biosensors have been widely used in various fields of detection and analysis due to their high sensitivity, superior selectivity, quick reaction time, and inexpensive cost. However, the signal changes caused by interactions between a biological probe and a target molecule are very weak and difficult to capture directly by using detection instruments. Therefore, various signal amplification strategies have been proposed and developed to increase the accuracy and sensitivity of detection systems. This review serves as a reference for biosensor and detector research, as it introduces the research progress of electrochemical signal amplification strategies in olfactory and taste evaluation. It also discusses the latest signal amplification strategies currently being employed in electrochemical biosensors for nanomaterial development, enzyme labeling, and nucleic acid amplification techniques, and highlights the most recent work in using cell tissues as biosensitive elements.

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Recent progress on microfluidic biosensors for rapid detection of pathogenic bacteria

Cited by 97 publications

References 83 publications

Aptamer-Based Lateral Flow Assays: Current Trends in Clinical Diagnostic Rapid Tests

Aptamer-Based Lateral Flow Assays: Current Trends in Clinical Diagnostic Rapid Tests

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Electrochemical Signal Amplification Strategies and Their Use in Olfactory and Taste Evaluation

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