Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview

Abstract

Escherichia coli O157:H7 (E. coli O157:H7) is a pathogenic strain of Escherichia coli which has issued as a public health threat because of fatal contamination of food and water. Therefore, accurate detection of pathogenic E. coli is important in environmental and food quality monitoring. In spite of their advantages and high acceptance, culture-based methods, enzyme-linked immunosorbent assays (ELISAs), polymerase chain reaction (PCR), flow cytometry, ATP bioluminescence, and solid-phase cytometry have various drawbacks, including being time-consuming, requiring trained technicians and/or specific equipment, and producing biological waste. Therefore, there is necessity for affordable, rapid, and simple approaches. Electrochemical biosensors have shown great promise for rapid food- and water-borne pathogen detection. Over the last decade, various attempts have been made to develop techniques for the rapid quantification of E. coli O157:H7. This review covers the importance of E. coli O157:H7 and recent progress (from 2015 to 2020) in the development of the sensitivity and selectivity of electrochemical sensors developed for E. coli O157:H7 using different nanomaterials, labels, and electrochemical transducers.

Review of recent advances in improved lateral flow immunoassay for the detection of pathogenic Escherichia coli O157:H7 in foods

“…To develop rapid, simple, sensitive, and/or cheaper methods for bacteria detection, electrochemical (Lin et al, 2008; Razmi, Hasanzadeh, Willander, & Nur, 2020; Xu, Wang, & Li, 2016), optical (Quintela et al, 2015; Taniguchi, Saito, & Mitsubayashi, 2017), microfluidic (Bianet et al, 2015), quartz crystal microbalance (Wu, Chen, & Lin, 2007), flow cytometry (Liu et al, 2019), and magnetoelastic biosensors (Jo, Park, & Park, 2020) have been investigated for pathogen detection in foods . Among those developed technologies, lateral flow immunoassay (LFIA) is the most practical and easy to be commercialized (Çam, 2019).…”

Electrochemical genosensor based on gold nanostars for the detection of Escherichia coli O157:H7 DNA

“…Enzyme-linked immunosorbent assay (ELISA) is an immunological technique designed for screening of the either antibody or antigen in a given sample [9][10][11][12] . In spite of some advantages, ELISA has certain limitations such as requiring well-trained staff, high cost, lengthy procedure steps and analysis time 3,6 . Therefore, there is necessity to develop a sensitive, selective, rapid, and simple detection method of E. coli O157:H7 in food industry, environmental monitoring, and clinical diagnosis.…”

Regenerable ZnO/GaAs Bulk Acoustic Wave Biosensor for Detection of Escherichia coli in “Complex” Biological Medium

“…The need for real-time detection has been a major driving force behind the emergence of biosensors as promising alternative platforms capable of rapid, sensitive and potentially cost-attractive detection of pathogenic bacteria [ 9 ]. Great effort has gone into developing new biosensors targeting E. coli [ 10 , 11 , 12 , 13 , 14 , 15 ] and limits of detection (LOD) as low as 1 CFU/mL have been reported for the detection of E. coli using different types of biosensors [ 16 , 17 , 18 ]. Despite the progress and the low LODs achieved in recent years, there is still no practical biosensor which could overall satisfy the market requirements, such as short analysis time, high sensitivity and aptitude for detection in complex media.…”