Recent progress on the recovery of bioactive compounds obtained from propolis as a natural resource: Processes, and applications

Contieri, Letícia S.; Mesquita, Leonardo M. de Souza; Sanches, Vitor L.; Chaves, Jaísa Oliveira; Pizani, Rodrigo S.; Silva, Laíse C. da; Viganó, Juliane; Ventura, Sónia P. M.; Rostagno, Maurı́cio A.

doi:10.1016/j.seppur.2022.121640

Cited by 14 publications

(8 citation statements)

References 130 publications

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“…For example, a serial of flavone and phenolic acid compounds, which are responsible for its physiological activities had better be monitored pre-or after the purification. [6] Currently, extraction, isolation and purification approaches for propolis are diverse, [7,8] including traditional organic solvents extraction, [9] subcritical water extraction, [10] deep eutectic solvents extraction, [11] macro-porous resin enrichment, [12] ultrasound extraction, [13] microwave assisted extraction [14] and supercritical fluid extraction. [15] In addition to these methods, membrane-based ultrafiltration is highly suitable for natural products as it does not require heating, preventing deactivation of many natural molecules.…”

Section: Introductionmentioning

confidence: 99%

Membrane‐Based Preparation Process and Antioxidant and Anti‐AGEs Activities of a Novel Propolis Ultrafiltrate

Liang,

Wu,

Chen

et al. 2024

Chemistry & Biodiversity

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Propolis is one functional supplement with hundreds of years of usage. However, it’s rarely consumed directly for its resinous property. Herein, a pre‐treated process which can remove the impurity while preserve its bioactivities is needed to maximise its therapeutic opportunities. In the present study, a membrane‐based ultrafiltration process was developed on a KM1812‐NF experimental instrument. Using Brazilian green propolis as testing material, all experimental steps and parameters were sequentially optimized. In addition, a mathematical model was developed to fit the process. As a result, the optimum solvent was 60% ethanol adjusted to pH 8‐9, while the optimum MWCO (molecular weight cut‐off) value of membrane was 30 KDa. The membrane filtration dynamic model fitted with the function y=(ax+b)/(1+cx+dx2). The resulting propolis ultrafiltrate from Brazilian green propolis, termed P30K, contains the similar profile of flavonoids and phenolic acids as raw propolis. Meanwhile, the ORAC (oxygen radical absorbance capacity) value of P30K is 11429.45±1557.58 µM TE/g and the IC50 value of inhibition of fluorescent AGEs (advanced glycation end products) formation is 0.064 mg/mL. Our work provides an innovative alternative process for extraction of active compounds from propolis and reveals P30K as an efficient therapeutic antioxidant.

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Section: Introductionmentioning

confidence: 99%

Membrane‐Based Preparation Process and Antioxidant and Anti‐AGEs Activities of a Novel Propolis Ultrafiltrate

Liang,

Wu,

Chen

et al. 2024

Chemistry & Biodiversity

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“…8 However, selecting the right propolis and extraction process is necessary due to variations in phenolic compounds present. 9,10 Thus, to develop an efficient extraction process while preserving their biological properties, it is crucial to use an appropriate solvent to solubilize the biomass and recover the desired compounds in a highperformance and sustainable operational mode. 11 Ethanol is the primary solvent used to produce propolis extracts on a large scale.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Initial Screening. The initial screening involved ultrasonicassisted extraction using an ultrasonic bath (P60H, Elmasonic, Singen, Germany, 2.75 L, 37 kHz, 135 W) under fixed operational conditions (namely, the solid−liquid ratio [R (S/L) ] of 0.015, meaning 0.015 g of propolis per mL of solvents for 120 min (min) at 55 °C), according to the method described by (Contieri et al, 2022). 29 After extraction, a 5 mL aliquot was centrifuged at 14,000 rpm and 20 °C for 15 min.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The European Food Safety Authority (EFSA) stated that establishing a relationship between propolis consumption and health effects is likely . However, selecting the right propolis and extraction process is necessary due to variations in phenolic compounds present. , Thus, to develop an efficient extraction process while preserving their biological properties, it is crucial to use an appropriate solvent to solubilize the biomass and recover the desired compounds in a high-performance and sustainable operational mode …”

Section: Introductionmentioning

confidence: 99%

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Unlocking the Full Potential of Green Propolis: A Novel Extraction Approach Using Eutectic Solvents for Improved Phenolic Compound Recovery

Contieri,

Ribeiro,

Sosa

et al. 2023

ACS Sustainable Chem. Eng.

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Propolis is highly diverse and has various health benefits for humans. However, standardizing propolis-based products for use in the pharmaceutical and food industries is challenging. Efficient extraction processes are essential to maximize their biological potential. Ethanol is commonly used as a solvent but has several drawbacks, such as residual flavor and limitations for some individuals. Water is an alternative, but it is not as effective as ethanol. In this context, new strategies are being explored to find an alternative to ethanol and improve water's performance as a solvent. To this end, a new extraction platform of phenolic compounds from Brazilian green propolis using eutectic solvents was developed. Betaine: citric acid (1:2) with 50 wt % of water was found to be the most efficient solvent, with a yield of extraction around 100 mg polyphenol g propolis −1 under optimum conditions with an ultrasound-assisted extraction approach (UAE). In vitro biological assays were performed to evaluate the potential of this new extract, mainly regarding antioxidant, antihypertensive, lipase inhibition, and antimicrobial activity. Our results indicate that the eutectic solvent selected in the extraction process enhances the biological potential of the green propolis extract, opening the doors for the development of semifinished products active against inflammation biological pathways.

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“…The use of propolis is widespread, and the interest in scientific research is increasing due to the potential source of various bioactive substances [1, 2]. Propolis comprises hydrophilic and hydrophobic molecules and is usually extracted with ethanol, the primary extraction solvent used by researchers and industry [3, 4]. The chemical composition of propolis depends on the geographic location, climate, edaphic conditions of the plants used by the bees (soil type, chemical composition, and microbiota) and by the bee species [5].…”

Section: Introductionmentioning

confidence: 99%

Ultra‐high‐performance liquid chromatography using a fused‐core particle column for fast analysis of propolis phenolic compounds

Contieri

Mesquita

Sanches

et al. 2022

J of Separation Science

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Propolis is a bee product with a complex chemical composition formed by several species from different geographical origins. The complex propolis composition requires an accurate and reproducible characterization of samples to standardize the quality of the material sold to consumers. This work developed an ultra-highperformance liquid chromatography with a photodiode array detector method to analyze propolis phenolic compounds based on the two key propolis biomarkers, Artepillin C and p-Coumaric acid. This choice was made due to the complexity of the sample with the presence of several compounds. The optimized method was hyphenated with mass spectrometry detection allowing the detection of 23 different compounds. A step-by-step strategy was used to optimize temperature, flow rate, mobile phase composition, and re-equilibration time. Reverse-phase separation was achieved with a C18 fused-core column packed with the commercially available smallest particles (1.3 nm). Using a fused-core column with ultra-high-performance liquid chromatography allows highly efficient, sensitive, accurate, and reproducible determination of compounds extracted from propolis with an outstanding sample throughput and resolution. Optimized conditions permitted the separation of the compounds in 5.50 min with a total analysis time (sample-to-sample) of 6.50 min.

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Recent progress on the recovery of bioactive compounds obtained from propolis as a natural resource: Processes, and applications

Cited by 14 publications

References 130 publications

Membrane‐Based Preparation Process and Antioxidant and Anti‐AGEs Activities of a Novel Propolis Ultrafiltrate

Membrane‐Based Preparation Process and Antioxidant and Anti‐AGEs Activities of a Novel Propolis Ultrafiltrate

Unlocking the Full Potential of Green Propolis: A Novel Extraction Approach Using Eutectic Solvents for Improved Phenolic Compound Recovery

Ultra‐high‐performance liquid chromatography using a fused‐core particle column for fast analysis of propolis phenolic compounds

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