Abstract.We present results of analysis of biological (diatoms and ostracodes) and non-biological (Ti, Ca / Ti, total inorganic carbon, magnetic susceptibility) variables from an 8.8 m long, high-resolution (∼ 20 yr sample −1 ) laminated sediment sequence from Lake Santa María del Oro (SMO), western Mexico. This lake lies at a sensitive location between the dry climates of northern Mexico, under the influence of the North Pacific subtropical high-pressure cell and the moister climates of central Mexico, under the influence of the seasonal migration of the intertropical convergence zone and the North American monsoon (NAM). The sequence covers the last 2000 years and provides evidence of two periods of human impact in the catchment, shown by increases in the diatom Achnanthidium minutissimum. The first from AD 100 to 400 (Early Classic) is related to the shaft and chamber tombs cultural tradition in western Mexico, and the second is related to Post-Classic occupation from AD 1100 to 1300. Both periods correspond to relatively wet conditions.