Recent Research Trends and Future Prospects in Nanozymes

Shin, Ho Yun; Park, Tae Jung; Kim, Moon Il

doi:10.1155/2015/756278

Cited by 65 publications

(34 citation statements)

References 83 publications

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“…From an analysis of dose-response curves using CoAC for glucose detection, the limit of detection for glucose was determined to be as low as 5 μM in the linear range from 10 μM to 1 mM ( Figure 6 b). These limit of detection (LOD) and linear range values are among the best results of those recently reported, describing the various enzyme mimetics-based colorimetric measurements of glucose [ 30 , 31 ].…”

Section: Resultsmentioning

confidence: 68%

Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection

Song

Lee

Bui

et al. 2018

Sensors

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In this study, we describe a novel peroxidase-like activity of Co-aminoclay [CoAC] present at pH ~5.0 and its application to fluorescent biosensor for the determination of H2O2 and glucose. It is synthesized with aminoclays (ACs) entrapping cationic metals such as Fe, Cu, Al, Co., Ce, Ni, Mn, and Zn to find enzyme mimicking ACs by sol–gel ambient conditions. Through the screening of catalytic activities by the typical colorimetric reaction employing 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid)diammonium salt (ABTS) as a substrate with or without H2O2, Fe, Cu, and CoACs are found to exhibit peroxidase-like activity, as well as oxidase-like activity was observed from Ce and MnACs. Among them, CoAC shows exceptionally high peroxidase-like activity, presumably due to its ability to induce electron transfer between substrates and H2O2. CoAC is then used to catalyze the oxidation of Amplex® UltraRed (AUR) into a fluorescent end product, which enables a sensitive fluorescent detection of H2O2. Moreover, a highly sensitive and selective glucose biosensing strategy is developed, based on enzyme cascade reaction between glucose oxidase (GOx) and CoAC. Using this strategy, a highly linear fluorescence enhancement is verified when the concentration of glucose is increased in a wide range from 10 μM to 1 mM with a lower detection limit of 5 μM. The practical diagnostic capability of the assay system is also verified by its use to detect glucose in human blood serum. Based on these results, it is anticipated that CoAC can serve as potent peroxidase mimetics for the detection of clinically important target molecules.

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Section: Resultsmentioning

confidence: 68%

Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection

Song

Lee

Bui

et al. 2018

Sensors

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“…However, these techniques are expensive and require specialized laboratory facilities [5]. Currently, big efforts are focused on the application of nanomaterials and their properties to overcome the drawbacks other methods have [6]. There are two main strategies.…”

Section: Introductionmentioning

confidence: 99%

Test-System for Bacteria Sensing Based on Peroxidase-Like Activity of Inkjet-Printed Magnetite Nanoparticles

et al. 2020

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Rapid detection of bacterial contamination is an essential task in numerous medical and technical processes and one of the most rapidly developing areas of nano-based analytics. Here, we present a simple-to-use and special-equipment-free test-system for bacteria detection based on magnetite nanoparticle arrays. The system is based on peroxide oxidation of chromogenic substrate catalyzed by magnetite nanoparticles, and the process undergoes computer-aided visual analysis. The nanoparticles used had a pristine surface free of adsorbed molecules and demonstrated high catalytic activities up to 6585 U/mg. The catalytic process showed the Michaelis–Menten kinetic with Km valued 1.22 mmol/L and Vmax of 4.39 µmol/s. The nanoparticles synthesized were used for the creation of inkjet printing inks and the design of sensor arrays by soft lithography. The printed sensors require no special equipment for data reading and showed a linear response for the detection of model bacteria in the range of 104–108 colony-forming units (CFU) per milliliter with the detection limit of 3.2 × 103 CFU/mL.

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“…They can be applied for diagnostic biomedical, biotechnological and industrial applications. [1][2][3] Nanoscale artificial enzymes are highly stable structures that can be synthesized at low cost with good potential as replacements for natural enzymes. Carbon nanofibers (CNFs) are carbon-based nanostructures that are examples of such structures which can be prepared by catalytic chemical vapor desorption (CCVD) and electrospinning.…”

Section: Introductionmentioning

confidence: 99%

<p>Thermodynamics and kinetic analysis of carbon nanofibers as nanozymes</p>

Bahreini

Movahedi

Peyvandi

et al. 2019

NSA

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Purpose: Evaluation of structural features, thermodynamics and kinetic properties of carbon nanofibers (CNFs) as artificial nanoscale enzymes (nanozyme). Methods: Synthesis of CNFs was done using chemical vapor deposition, and transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM) and energy-dispersive x-ray spectroscopy (EDX) were used to provide information on the morphology, elemental monitoring and impurity assay of the CNFs. The thermal features of the CNFs were evaluated using differential thermal analysis (DTA), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) derivative and TGA. The calculated thermo-physical parameters were melting temperature (Tm), weight loss maximum temperature (T max) and enthalpy of fusion (ΔH fusion). Catalytic activity was assayed by a 4-aminoantypyrine (4-AAP)-H 2 O 2 coupled colorimetric system by UVvisible spectroscopy. Results: FE-SEM and TEM analysis demonstrated parallel graphitic layers and uniformity of atomic orientation and morphology. The EDX spectra approved carbon element as major signal and presence of partial Ti as impurities of CNFs during CVD process. The DTA thermogram showed the endothermic process had a maximum temperature of 82.27°C at −15.48 mV and that thermal decomposition occurred at about 200°C. The TGA-differential gravimetric analysis thermogram showed that T max was 700°C. The DSC heat flow curve showed a melting temperature (Tm) of 254.52°C, ΔH fusion of 3.84 J^.g −1 , area under the curve of 58.58 mJ and T e (onset) and T f (end set) temperatures of 246.60°C and 285.67°C, respectively. The peroxidase activity of the CNFs obeyed the Michaelis-Menten equation with a double-reciprocal curve and the calculated K m , K cat and V max kinetic parameters. Conclusion: CNFs as peroxidase nanozymes are intrinsically strong and stable nanocatalysts under difficult thermal conditions. The peroxidase activity was demonstrated, making these CNFs candidates for analytical tools under extreme conditions.

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Recent Research Trends and Future Prospects in Nanozymes

Cited by 65 publications

References 83 publications

Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection

Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection

Test-System for Bacteria Sensing Based on Peroxidase-Like Activity of Inkjet-Printed Magnetite Nanoparticles

<p>Thermodynamics and kinetic analysis of carbon nanofibers as nanozymes</p>

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