Recent Results of Single‐Molecule Spectroscopy in Solids

Kador, Lothar

doi:10.1002/pssb.2221890103

Cited by 41 publications

(10 citation statements)

References 75 publications

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“…Over the last decade considerable improvements have been made in the field of single molecule detection (SMD) by the use of fluorescence. Detection and spectroscopy of single molecules has been demonstrated in solids at cryo-temperatures [1,2] (for a review see [3]) and in liquids at room temperatures which is of particular interest to biomedical sciences and analytical chemistry [4][5][6][7][8][9][10][11] (for a review see [12]). Of crucial importance to SMD in liquids is, first of all, the achievement of a tight spatial as well as spectral filtering.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of photobleaching investigated by fluorescence correlation spectroscopy

Widengren¹,

Rigler²

1996

Bioimaging

149

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Fluorescence correlation spectroscopy (FCS) can be used to investigate the photobleaching properties of fluorophores in solution. The advantage with this method is that in addition to the photobleaching rate the formation and decay rates of the triplet state can be measured. In this way, it is possible to calculate the photodestruction quantum yield and relate the photostability of a fluorescent compound in a certain environment to the photodynamical behaviour of the singlet‐triplet transitions. This is likely to contribute to a better understanding of the mechanisms of photobleaching given the central importance of dye triplet states in photobleaching processes. The approach was applied to the measurement and characterization of the photobleaching of Rh6G in aqueous solution and FITC in 1 mM sodium carbonate buffer (pH 9). The photobleaching yields measured are discussed in view of the simultaneous triplet properties at different excitation intensities, oxygen concentrations as well as in the presence or absence of quencher molecules. This study suggests that FCS is likely to provide a valuable tool for the elucidation of the mechanisms of photobleaching, which are far from understood in all their details.

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Section: Introductionmentioning

confidence: 99%

Mechanisms of photobleaching investigated by fluorescence correlation spectroscopy

Widengren¹,

Rigler²

1996

Bioimaging

149

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“…In recent years, the optical spectroscopy of single organic molecules , in low-temperature solids has developed into an extremely sensitive tool for probing the properties of the molecules themselves as well as of the closely surrounding solids. Several reviews − on the different physical phenomena observed on single molecules have been published. The basic principles of the method are simple: Fluorescence excitation spectra are measured by scanning a single-mode laser across the region of the inhomogeneously broadened absorption band at liquid helium temperatures and by detecting the signal with high numerical aperture (N.A.)…”

Section: Introductionmentioning

confidence: 99%

Single-Molecule Spectroscopy of Benzodiphenanthrobisanthene in a Shpolskii Matrix

Vácha¹,

Tani²

1997

J. Phys. Chem. A

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We present a new system for single-molecule detection at cryogenic temperatures, benzodiphenanthrobisanthene in a Shpolskii matrix of n-tetradecane. Most of the single-molecule lines are broader than the lifetimelimited value of 31.0 MHz, indicating the presence of spectral diffusion in the crystalline sample. For the detection of the fluorescence signal, we use a gradient index lens in a back-illumination configuration.

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“…The shape of such a band (inhomogeneous distribution function) in a glass is approximately Gaussian (Stoneham (1969), Kador (1991)). The main distinction of amorphous solids is the absence of long-range order.…”

Section: Amorphous Solidsmentioning

confidence: 99%

“…Hole-Burning Spectroscopy (H. Maier et al) pared to their distance. The diffusional kernel for optical spectra at arbitrary TS concentrations was calculated by Kador (1991) (a criterion for the limiting cases of "high" and "low" concentrations is also given in this reference). The dependence of the diffusion kernel on the interaction mechanism is not completely clear.…”

Section: Theoretical Description Of Spectral Diffusionmentioning

confidence: 99%