The interest in the fermentative production of lactic acid has increased due to the prospects of environmental friendliness and of using renewable resources instead of petrochemicals. In this context, we have judged that is gainful to use the date juice which is with a low commercial value as a substrate for the production of lactic acid by Streptococcus thermophilus. Further, dates are rich in sugar ranging from 65% to 80% on dry weight basis mostly of inverted form (glucose and fructose), which can used as carbon source for the fermentation and the production of microbial biomass. The parameters optimization for lactic acid production by S. thermophilus grown on medium based on dates in order to improve yields is applied in this study. The results showed that the maximum of lactic acid production (36.9 g/l) is obtained after the enrichment of date juice with yeast extract (15%), Tween 80 (1g / l) MgSO 4 (0.5g/l), MnSO 4 (0.02g/l) and K 2 HPO 4 (1g/l).
Keywords:Lactic acid production; Date juice; Streptococcus thermophilus in the food, cosmetic, pharmaceutical, and chemical industries and has received increased attention for use as a precursor for the biodegradable plastic polylactic acid, used in medical, industrial, and consumer products [2]. Lactic acid can be produced commercially by either chemical synthesis or microbial fermentation [3]. The production by fermentation process has attracted interest due to its advantages compared with chemical synthesis such as production of pure isomers (L(+) or D(-) lactic acid), use of renewable and low cost raw materials, low energy consumption and mild operation conditions [4]. There are many cheap and renewable raw materials in the form of starch (corn, wheat, potato, cassava, rice, sweet sorghum) and lignocelluloses (corn cobs, waste paper and woody materials) can be used as a substrate for fermentation of lactic acid [5]. Renewable resources do not give any net contribution of carbon dioxide to the atmosphere, as do the limited oil and fossil-fuel-based sources. Lactic acid can be biosynthesized by some bacteria and filamentous fungi [4].Algeria have a rich biodiversity of about 1900 date cultivars, but 30% of the production of dates is characterized by a low commercial quality [6]. Beside direct consumption of the whole dates, the fruits are traditionally used to prepare a wide range of product such as date juice concentrates (spread, syrup and liquid sugar), fermented date products (vinegar, organic acids) and date pastes for different uses e.g. bakery and confectionary. Date fruits are considered as a good source of sugars. It provides natural sugar in the form of glucose and fructose [7], which could be used as carbonaceous source of fermentation for the production of the lactic acid. The aim of the present study is the valorisation of the dates by production of its juice and used it as medium for the development of S. thermophillus and the production of lactic acid.
Materials and Methods
Plant materialThe date palm of Ghars cultivars grown in Bechar, S...