Recent trends in metal‐organic frameworks mediated lipase immobilization: A state‐of‐the‐art review

Abstract

Immobilized lipase is a powerful biocatalytic system with numerous applications in industries, particularly in the energy, pharmaceutical, cosmetic, and food industries. Reusability, simple recovery, and high chemical and thermal stability make it an attractive alternative to traditional chemical catalysts in industrial applications. Novel methods and support materials for immobilizing lipases have recently attracted much attention. Metal‐organic frameworks (MOFs) are a promising class of materials for enzyme immobilization carriers due to their appealing features, including a high specific surface area, high specific porosity, a stable framework structure, and a wide variety of functional sites. Due to the protection provided to enzymes by MOFs, several reported MOFs‐lipase composites display exceptional catalytic characteristics relative to free lipases. This includes increased enzyme efficiency, stability, selectivity, and recyclability. Herein, we summarize an updated review of the most recent advances in MOFs immobilizing lipases. This review sheds light on the numerous aspects of lipase‐MOF immobilization, with special emphasis on different techniques of designing lipase‐MOF platforms and the advantages of lipase‐MOF composites. Subsequently, molecular simulation approaches in lipase‐MOF immobilization are briefly introduced. Moreover, practical applications of MOFs‐lipase composites have been outlined. Finally, potential limitations and future directions for MOFs‐lipase immobilization research are highlighted.

Preparation of Proline-Modified UIO−66 Nanomaterials and Investigation of Their Potential in Lipase Immobilization

“…Among the most popular enzymes, lipases (EC 3.1.1.3) have enormous biocatalytic potential in numerous areas of industrial microbiology and biotechnology and are today considered an integral part of a wide variety of industrial processes [1,2]. Lipase-catalyzed processes encompass esterification, inter-esterification, hydrolysis, alcholysis, acidolysis, and aminolysis [3].…”

“…Metal-organic frameworks (MOFs) are a type of structured crystalline solid that is created by linking metal ions or metal clusters with organic linkers [17,18]. In recent years, MOF materials have exhibited substantial promise in numerous areas of research, including catalysis, drug delivery, biosensing, gas storage and separation, food science, biodiesel synthesis, and many more [1,2]. Due to its substantial specific surface area, straightforward synthesis and modification process, and favorable physicochemical features, it has been regarded as a viable material for lipase immobilization [19,20].…”