Recent Trends in Microbiological Safety of Fruits and Vegetables

Buck, James W.; Walcott, R. R.; Beuchat, Larry R.

doi:10.1094/php-2003-0121-01-rv

Cited by 203 publications

(161 citation statements)

References 49 publications

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“…Human contact and carriers of S. aureus help in its transfer unto vegetables, especially those vegetables hawked or exposed along roadsides. Outbreaks caused by Staphylococcus have implicated vegetables like carrots, alfalfa sprouts, lettuce, parsley, radish and onion sprouts as vehicles [9]. Carrots ready-for-consumption in this part of the world are majorly exposed along roadsides or hawked while exposed in streets or markets.…”

Section: Discussionmentioning

confidence: 99%

“…There is an inadequate awareness of the plant-microbe interactions, epiphytic and immigrant micro-organism on leaves and phyllosphere of vegetables [12] which is prompting the continuous spread of pathogens to humans. Many sources of contamination of vegetables include soil, faeces, water for irrigation, dust, insects, inadequately composted manure, wild or domestic animal wastes, and human handling [9]. The use of inadequately treated water for irrigation purpose in carrot cultivation could increase the spread of E. coli and other enteropathogens [12].…”

Section: Discussionmentioning

confidence: 99%

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Escherichia coli and Staphylococcus aureus Contaminations of Carrots Sold within Zaria, Nigeria and their Antibiotic Susceptibility Profiles

Bishop¹,

Okwori²

2017

OAJS

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Escherichia coli and Staphylococcus aureus Contaminations of Carrots Sold within Zaria, Nigeria and their Antibiotic Susceptibility Profiles

Bishop¹,

Okwori²

2017

OAJS

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“…Despite these foods being ready to eat, it has been reported that their quality is not satisfactory in Vienna, Austria [23], Johannesburg, South Africa [24], Korea [25], and Catalonia, Spain [26]. Reports show that the main pathogens in ready-to-eat foods include Listeria monocytogenes, S. aureus, Bacillus cereus, Salmonella spp., and Escherichia coli O157:H7, the last two being involved in most outbreaks caused by fresh fruits and vegetables [27,28] reported in low doses of 10 and 2-2000 cells, respectively [29,30].…”

Section: Staphylococcal Intoxicationmentioning

confidence: 99%

PCR Assay for Detection of Staphylococcus aureus in Fresh Lettuce (Lactuca sativa)

Chávez‐Almanza¹,

López‐Cervantes²,

Cantú-Soto³

et al. 2017

Frontiers in Staphylococcus Aureus

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The growth in food demand and production growth of vegetables have led to the development of intensive production systems with the aim of having regular access to enough high-quality food. The aim is to determine the incidence of Staphylococcus aureus in fresh letuce by PCR in order to enhance the eiciency for detection and identiication process. The Baird-Parker method was used for isolating pathogens from 54 letuce samples. Genomic DNA extraction was performed according the Mericon DNA Bacteria Plus Kit. The detection by PCR was performed using the pair of primers: coa gene (5′-ATAGAGCTGATGGTACAGG-3′ and 5′-GCTTCCGATTGTTCGATGC-3′). The phylogenetic tree was constructed by comparing conserved sequences from the adjacent 16S gene, using the F2C 5′-AGAGTTTGATCATGGCTC-3′ and C 5′-ACGGGCGGTGTGTAC-3′ primers. To test the antimicrobial efect, we used the disk difusion method (Kirby-Bauer) using Mueller-Hinton agar and ive antibiotics with diferent concentrations. The incidence of S. aureus was 1.7%. All the isolates were situated in the ATCC 11632 clade in accordance with other reported sequences belonging to this pathogen in the NCBI database. All the isolates seemed to be resistant to penicillin (10U). The molecular techniques used in this study are suitable for the identiication of S. aureus isolated from letuce, increasing our capability of detecting this pathogen by improving the process and increasing the eiciency contributing to the safety of this vegetable.

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“…Fresh produce can become contaminated with pathogens during growth, harvesting, packaging, or food preparation through various routes, such as manure compost being used as a soil amendment for soil quality, irrigation water, or fecal contamination by active wildlife or proximity to livestock in the field [6][7][8][9]. Data on the source of outbreaks shows that the majority of outbreaks related to fresh produce have mostly been caused by zoonotic bacterial pathogens of animal origin such as Salmonella and E. coli O157:H7 [2].…”

Section: Introductionmentioning

confidence: 99%

Potential Application of Fluorescence Imaging for Assessing Fecal Contamination of Soil and Compost Maturity

Cho

Lee

Kim³

et al. 2016

Applied Sciences

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Pathogenic microorganisms can lead to serious outbreaks of foodborne illnesses, particularly if fresh produce becomes contaminated and then happens to be inappropriately handled in a manner that can incubate pathogens. Pathogenic microbial contamination of produce can occur through a variety of pathways, such as from the excrement of domesticated and wild animals, biological soil amendment, agricultural water, worker health and hygiene, and field tools used during growth and harvest. The use of mature manure compost and preventative control of fecal contamination from wildlife and livestock are subject to safety standards to minimize the risk of foodborne illness associated with produce. However, in a field production environment, neither traces of animal feces nor the degree of maturity of manure compost can be identified by the naked eye. In this study, we investigated hyperspectral fluorescence imaging techniques to characterize fecal samples from bovine, swine, poultry, and sheep species, and to determine feasibilities for both detecting the presence of animal feces as well as identifying the species origin of the feces in mixtures of soil and feces. In addition, the imaging techniques were evaluated for assessing the maturity of manure compost. The animal feces exhibited dynamic and unique fluorescence emission features that allowed for the detection of the presence of feces and showed that identification of the species origin of fecal matter present in soil-feces mixtures is feasible. Furthermore, the results indicate that using simple single-band fluorescence imaging at the fluorescence emission maximum for animal feces, simpler than full-spectrum hyperspectral fluorescence imaging, can be used to assess the maturity of manure compost.

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Recent Trends in Microbiological Safety of Fruits and Vegetables

Cited by 203 publications

References 49 publications

Escherichia coli and Staphylococcus aureus Contaminations of Carrots Sold within Zaria, Nigeria and their Antibiotic Susceptibility Profiles

Escherichia coli and Staphylococcus aureus Contaminations of Carrots Sold within Zaria, Nigeria and their Antibiotic Susceptibility Profiles

PCR Assay for Detection of Staphylococcus aureus in Fresh Lettuce (Lactuca sativa)

Potential Application of Fluorescence Imaging for Assessing Fecal Contamination of Soil and Compost Maturity

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