Recent trends in protein and peptide-based biomaterials for advanced drug delivery

Varanko, Anastasia Kristine; Saha, Soumen; Chilkoti, Ashutosh

doi:10.1016/j.addr.2020.08.008

Cited by 233 publications

(143 citation statements)

References 501 publications

(718 reference statements)

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“…In addition, protein and peptide-based systems were used to reprogram somatic cells to iPSCs [ 95 , 96 , 97 , 98 ]. Nanoparticles were used for the encapsulation and conjugation of the peptides for delivery and functional integration [ 99 , 100 , 101 , 102 ]. The combination of peptide-based system and nanoparticles will provide a new powerful tool for iPSC generation and application.…”

Section: Nanoparticles In Ipsc Generation and Precision Medicinementioning

confidence: 99%

Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles

2021

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Human induced pluripotent stem cells (HiPSCs) and HiPSCs-derived cells have the potential to revolutionize regenerative and precision medicine. Genetically reprograming somatic cells to generate HiPSCs and genetic modification of HiPSCs are considered the key procedures for the study and application of HiPSCs. However, there are significant technical challenges for transgene delivery into somatic cells and HiPSCs since these cells are known to be difficult to transfect. The existing methods, such as viral transduction and chemical transfection, may introduce significant alternations to hiPSC culture which affect the potency, purity, consistency, safety, and functional capacity of HiPSCs. Therefore, generation and genetic modification of HiPSCs through non-viral approaches are necessary and desirable. Nanotechnology has revolutionized fields from astrophysics to biology over the past two decades. Increasingly, nanoparticles have been used in biomedicine as powerful tools for transgene and drug delivery, imaging, diagnostics, and therapeutics. The most successful example is the recent development of SARS-CoV-2 vaccines at warp speed to combat the 2019 coronavirus disease (COVID-19), which brought nanoparticles to the center stage of biomedicine and demonstrated the efficient nanoparticle-mediated transgene delivery into human body. Nanoparticles have the potential to facilitate the transgene delivery into the HiPSCs and offer a simple and robust approach. Nanoparticle-mediated transgene delivery has significant advantages over other methods, such as high efficiency, low cytotoxicity, biodegradability, low cost, directional and distal controllability, efficient in vivo applications, and lack of immune responses. Our recent study using magnetic nanoparticles for transfection of HiPSCs provided an example of the successful applications, supporting the potential roles of nanoparticles in hiPSC biology. This review discusses the principle, applications, and significance of nanoparticles in the transgene delivery to HiPSCs and their successful application in the development of COVID-19 vaccines.

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Section: Nanoparticles In Ipsc Generation and Precision Medicinementioning

confidence: 99%

Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles

2021

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“…However, prediction of the supramolecular behavior of SAPs is a challenge because a large number of factors are involved in the self-assembling process [ 62 ]. Nevertheless, fine control over the process allows for innovative solutions in drug delivery [ 63 ], which included the delivery of proteins [ 64 , 65 ] and peptide-based therapeutics [ 66 , 67 ], tissue engineering [ 68 , 69 ], biomimicry [ 70 ], cancer cell detection [ 71 ], and even vaccine adjuvants to stimulate the immune response [ 72 ], through their use as emulsifiers [ 73 ], pigments [ 74 ], catalysts [ 75 ], and semiconductors [ 76 ]. Several studies have demonstrated the crucial role of the SAP secondary structure to control the supramolecular architecture [ 66 ].…”

Section: Introductionmentioning

confidence: 99%

Peptide–Protein Interactions: From Drug Design to Supramolecular Biomaterials

2021

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The self-recognition and self-assembly of biomolecules are spontaneous processes that occur in Nature and allow the formation of ordered structures, at the nanoscale or even at the macroscale, under thermodynamic and kinetic equilibrium as a consequence of specific and local interactions. In particular, peptides and peptidomimetics play an elected role, as they may allow a rational approach to elucidate biological mechanisms to develop new drugs, biomaterials, catalysts, or semiconductors. The forces that rule self-recognition and self-assembly processes are weak interactions, such as hydrogen bonding, electrostatic attractions, and van der Waals forces, and they underlie the formation of the secondary structure (e.g., α-helix, β-sheet, polyproline II helix), which plays a key role in all biological processes. Here, we present recent and significant examples whereby design was successfully applied to attain the desired structural motifs toward function. These studies are important to understand the main interactions ruling the biological processes and the onset of many pathologies. The types of secondary structure adopted by peptides during self-assembly have a fundamental importance not only on the type of nano- or macro-structure formed but also on the properties of biomaterials, such as the types of interaction, encapsulation, non-covalent interaction, or covalent interaction, which are ultimately useful for applications in drug delivery.

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“…In regenerative medicine, restoration of biological function can be aided by biomaterials created from metals, ceramics or polymers offering a variety of chemical structures, surface properties, degradation rates, and mechanical stiffness [ 1 , 2 ]. These properties allow for material functionalization to promote cell adhesion, support cell differentiation, exert anti-microbial effects, and assist drug delivery, among other applications [ 3 , 4 , 5 ]. Phosphate based glasses (PBGs) are biomaterials of particular interest due to their complete yet controllable resorption profiles which can be modified by simply altering the materials composition [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses

Melo

Murrell

Islam

et al. 2021

IJMS

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Phosphate-based glasses (PBGs) offer significant therapeutic potential due to their bioactivity, controllable compositions, and degradation rates. Several PBGs have already demonstrated their ability to support direct cell growth and in vivo cytocompatibility for bone repair applications. This study investigated development of PBG formulations with pyro- and orthophosphate species within the glass system (40 − x)P2O5·(16 + x)CaO·20Na2O·24MgO (x = 0, 5, 10 mol%) and their effect on stem cell adhesion properties. Substitution of phosphate for calcium revealed a gradual transition within the glass structure from Q2 to Q0 phosphate species. Human mesenchymal stem cells were cultured directly onto discs made from three PBG compositions. Analysis of cells seeded onto the discs revealed that PBG with higher concentration of pyro- and orthophosphate content (61% Q1 and 39% Q0) supported a 4.3-fold increase in adhered cells compared to glasses with metaphosphate connectivity (49% Q2 and 51% Q1). This study highlights that tuning the composition of PBGs to possess pyro- and orthophosphate species only, enables the possibility to control cell adhesion performance. PBGs with superior cell adhesion profiles represent ideal candidates for biomedical applications, where cell recruitment and support for tissue ingrowth are of critical importance for orthopaedic interventions.

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Recent trends in protein and peptide-based biomaterials for advanced drug delivery

Cited by 233 publications

References 501 publications

Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles

Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles

Peptide–Protein Interactions: From Drug Design to Supramolecular Biomaterials

Tailoring Pyro- and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses

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