Recent updates in the development of molecular assays for the rapid identification and susceptibility testing of MRSA

Mizusawa, Masako; Carroll, Karen C.

doi:10.1080/14737159.2023.2234823

Cited by 3 publications

(1 citation statement)

References 179 publications

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“…Furthermore, not all pathogens can grow on traditional culture methods, leading to culture-negative results with estimates ranging from 14% to 45%. Multiple bacteria may be present in the PJI microbiome, and the dominant bacteria may overwhelm the other organisms [ 4 , 9 ]. Next-generation DNA sequencing (NGS) has been previously used in PJI to address the issue of culture-negative results [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Culture Negative Pasteurella multocida Confirmed Prosthetic Hip Infection using Next-generation Sequencing

Kuechly,

Gupta,

Kurkowski

et al. 2024

jocr

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Introduction: Prosthetic joint infections (PJIs) are a dreaded complication of joint arthroplasty. Zoonotic organisms such as Pasteurella multocida (PM) rarely cause PJIs. Still, these organisms can be challenging to treat due to a low suspicion index and inadequate growth on culture. Next-generation sequencing (NGS) can be used to identify organisms in culture-negative PJIs. This is the first reported case of a PM positive total hip arthroplasty PJI using NGS. Case Report: We report the case of a 70-year-old male presenting with a periprosthetic hip infection. PM was identified in high relative abundance on NGS and grew in culture. Subsequent intraoperative samples were culture negative for Pasteurella, but NGS demonstrated continued presence of Pasteurella. Conclusion: PM is a rare case of PJI, but a high index of suspicion must be maintained in the appropriate clinical context. NGS is a vital tool for the identification of culture-negative organisms like PM. Keywords: Prosthetic joint infection, next-generation sequencing×culture negative, Pasteurella multocida, next-generation sequencing, hip infection, arthroplasty.

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Section: Introductionmentioning

confidence: 99%