Recently identified microbial guild mediates soil N2O sink capacity

Jones, Christopher M.; Spor, Aymé; Brennan, Fiona; Breuil, Marie-Christine; Bru, David; Lemanceau, Philippe; Griffiths, Bryan S.; Hallin, Sara; Philippot, Laurent

doi:10.1038/nclimate2301

Cited by 402 publications

(346 citation statements)

References 30 publications

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“…However, dissociation curve analysis following quantitative real-time PCR (qPCR) showed that there was sometimes minor unspecific coamplification, suggesting that the abundance of nosZ II organisms may be overestimated in some cases. The nosZ N 2 O reducers are critical in determining the soil N 2 O sink capacity (47), and conceivably the abundance of nosZ genes and/or mRNA should be correlated negatively with N 2 O emissions (48). However, no such correlation was found in the present study, suggesting that the correlation between nosZ genes and N 2 O emissions is more complex.…”

Section: Discussionmentioning

confidence: 99%

Microbial N Transformations and N ₂ O Emission after Simulated Grassland Cultivation: Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)

Duan

Kong

Schramm

et al. 2017

Appl Environ Microbiol

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Grassland cultivation can mobilize large pools of N in the soil, with the potential for N leaching and N 2 O emissions. Spraying with the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) before cultivation was simulated by use of soil columns in which the residue distribution corresponded to plowing or rotovation to study the effects of soil-residue contact on N transformations. DMPP was sprayed on aboveground parts of ryegrass and white clover plants before incorporation. During a 42-day incubation, soil mineral N dynamics, potential ammonia oxidation (PAO), denitrifying enzyme activity (DEA), nitrifier and denitrifier populations, and N 2 O emissions were investigated. The soil NO 3 Ϫ pool was enriched with 15 N to trace sources of N 2 O. Ammonium was rapidly released from decomposing residues, and PAO was stimulated in soil near residues. DMPP effectively reduced NH 4 ϩ transformation irrespective of residue distribution. Ammonia-oxidizing archaea (AOA) and bacteria (AOB) were both present, but only the AOB amoA transcript abundance correlated with PAO. DMPP inhibited the transcription of AOB amoA genes. Denitrifier genes and transcripts (nirK, nirS, and clades I and II of nosZ) were recovered, and a correlation was found between nirS mRNA and DEA. DMPP showed no adverse effects on the abundance or activity of denitrifiers. The 15 N enrichment of N 2 O showed that denitrification was responsible for 80 to 90% of emissions. With support from a control experiment without NO 3 Ϫ amendment, it was concluded that DMPP will generally reduce the potential for leaching of residue-derived N, whereas the effect of DMPP on N 2 O emissions will be significant only when soil NO 3 Ϫ availability is limiting.IMPORTANCE Residue incorporation following grassland cultivation can lead to mobilization of large pools of N and potentially to significant N losses via leaching and N 2 O emissions. This study proposed a mitigation strategy of applying 3,4-dimethylpyrazole phosphate (DMPP) prior to grassland cultivation and investigated its efficacy in a laboratory incubation study. DMPP inhibited the growth and activity of ammonia-oxidizing bacteria but had no adverse effects on ammonia-oxidizing archaea and denitrifiers. DMPP can effectively reduce the potential for leaching of NO 3 Ϫ derived from residue decomposition, while the effect on reducing N 2 O emissions will be significant only when soil NO 3 Ϫ availability is limiting. Our findings provide insight into how DMPP affects soil nitrifier and denitrifier populations and have direct implications for improving N use efficiency and reducing environmental impacts during grassland cultivation.

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Section: Discussionmentioning

confidence: 99%

Microbial N Transformations and N ₂ O Emission after Simulated Grassland Cultivation: Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)

Duan

Kong

Schramm

et al. 2017

Appl Environ Microbiol

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“…This implies that soil N 2 O emissions are predominantly determined by nirK-, rather than by nirS-type microorganisms. Thus, the nirS/nirK ratio may be negatively correlated with the soil N 2 O sink capacity [58,59]. Therefore, the increased nirK gene abundance in combination with the consistent or decreased amounts of nirS and nosZ genes can be seen as a predictor of enhanced potential for N 2 O emissions from infested forest sites.…”

Section: The Varying Effects On Denitrification Genesmentioning

confidence: 99%

The Abundance of Fungi, Bacteria and Denitrification Genes during Insect Outbreaks in Scots Pine Forests

Grüning

Beule

Meyer

et al. 2018

Forests

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Abstract:Outbreaks of defoliating insects may affect microbial populations in forests and thereby mass balances and ecosystem functioning. Here, we investigated the microbial dynamics in Scots pine (Pinus sylvestris L.) forests during outbreaks of the nun moth (Lymantria monacha L.) and the pine-tree lappet (Dendrolimus pini L.). We used real-time PCR (polymerase chain reaction) to quantify genes that characterize bacterial and fungal abundance and the denitrification processes (nirK, nirS, nosZ clades I and II) in different forest compartments and we analyzed the C and N content of pine needles, insect feces, larvae, vegetation layers, organic layers, and mineral soil horizons. The infestation of the nun moth increased the bacterial abundance on pine needles, in the vegetation layer, and in the upper organic layer, while fungal populations were increased in the vegetation layer and upper organic layer during both outbreaks. In soil, the abundance of nirK increased after insect defoliation, while the C/N ratios decreased. nosZ clades I and II showed variable responses in different soil layers and to different defoliating insects. Our results illustrate changes in the microbial populations in pine forests that were infested by defoliating insects and changes in the chemical soil properties that foster these populations, indicating a genetic potential for increased soil N 2 O emissions during the defoliation peak of insect outbreak events.

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“…Proxies for the capacity of soils to store/lose C and to enhance/reduce GHGs emissions can help to make choices on land use and soil management Soil C saturation capacity (Angers et al, 2011;Wiesmeier et al, 2014) has been proposed to estimate the potential gain of soil organic C. Its relevance for predicting soil C storage remains to be evaluated (O'Rourke et al, 2015;Dignac et al, 2017) Physical fractionation of soil organic C (particulate organic matter fraction) and thermal analysis have been proposed to estimate the potential for soil C loss (Dignac et al, 2017 and references therein) Soil capacity to reduce N 2 O (Hénault et al, 2001;Jones et al, 2014) Guidelines to estimate GHGs emissions from soil using models…”

Section: Resultsmentioning

confidence: 99%

Accounting for Carbon Stocks in Soils and Measuring GHGs Emission Fluxes from Soils: Do We Have the Necessary Standards?

Bispo

Andersen

Angers

et al. 2017

Front. Environ. Sci.

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Soil is a key compartment for climate regulation as a source of greenhouse gases (GHGs) emissions and as a sink of carbon. Thus, soil carbon sequestration strategies should be considered alongside reduction strategies for other greenhouse gas emissions. Taking this into account, several international and European policies on climate change are now acknowledging the importance of soils, which means that proper, comparable and reliable information is needed to report on carbon stocks and GHGs emissions from soil. It also implies a need for consensus on the adoption and verification of mitigation options that soil can provide. Where consensus is a key aspect, formal standards and guidelines come into play. This paper describes the existing ISO soil quality standards that can be used in this context, and calls for new ones to be developed through (international) collaboration. Available standards cover the relevant basic soil parameters including carbon and nitrogen content but do not yet consider the dynamics of those elements. Such methods have to be developed together with guidelines consistent with the scale to be investigated and the specific use of the collected data. We argue that this standardization strategy will improve the reliability of the reporting procedures and results of the different climate models that rely on soil quality data.

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Recently identified microbial guild mediates soil N2O sink capacity

Cited by 402 publications

References 30 publications

Microbial N Transformations and N ₂ O Emission after Simulated Grassland Cultivation: Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)

Microbial N Transformations and N ₂ O Emission after Simulated Grassland Cultivation: Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)

The Abundance of Fungi, Bacteria and Denitrification Genes during Insect Outbreaks in Scots Pine Forests

Accounting for Carbon Stocks in Soils and Measuring GHGs Emission Fluxes from Soils: Do We Have the Necessary Standards?

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Recently identified microbial guild mediates soil N2O sink capacity

Cited by 402 publications

References 30 publications

Microbial N Transformations and N 2 O Emission after Simulated Grassland Cultivation: Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)

Microbial N Transformations and N 2 O Emission after Simulated Grassland Cultivation: Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)

The Abundance of Fungi, Bacteria and Denitrification Genes during Insect Outbreaks in Scots Pine Forests

Accounting for Carbon Stocks in Soils and Measuring GHGs Emission Fluxes from Soils: Do We Have the Necessary Standards?

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Product

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Microbial N Transformations and N ₂ O Emission after Simulated Grassland Cultivation: Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)

Microbial N Transformations and N ₂ O Emission after Simulated Grassland Cultivation: Effects of the Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate (DMPP)