Receptor component protein (RCP): a member of a multi-protein complex required for G-protein-coupled signal transduction

Prado, Marcelo Alexandre; Evans-Bain, B.; Dickerson, Ian M.

doi:10.1042/bst0300460

Cited by 52 publications

(35 citation statements)

References 1 publication

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“…How CLR accessory proteins such as RCP and RAMPs promote CLR functions remains uncertain; RCP may couple the receptor to the G s protein, or it may activate adenylate cyclase directly (Prado et al, 2002). In mammals, RCP expression largely mirrors that of CLR (Ma et al, 2003), whereas RAMP expression typically exceeds that of CLR.…”

Section: Discussionmentioning

confidence: 99%

“…When expressed in NIH 3T3 cells, CG17415 did respond to DH 31 without co-transfection of RCP or RAMPs; this can be attributed to higher endogenous levels of RCP and RAMPs (Prado et al, 2002). How CLR accessory proteins such as RCP and RAMPs promote CLR functions remains uncertain; RCP may couple the receptor to the G s protein, or it may activate adenylate cyclase directly (Prado et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

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A novel diuretic hormone receptor inDrosophila: evidence for conservation of CGRP signaling

Johnson

Shafer

Trigg

et al. 2005

Journal of Experimental Biology

227

142

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SUMMARY The Drosophila orphan G protein-coupled receptor encoded by CG17415 is related to members of the calcitonin receptor-like receptor (CLR) family. In mammals, signaling from CLR receptors depend on accessory proteins, namely the receptor activity modifying proteins (RAMPs)and receptor component protein (RCP). We tested the possibility that this Drosophila CLR might also require accessory proteins for proper function and we report that co-expression of the mammalian or Drosophila RCP or mammalian RAMPs permitted neuropeptide diuretic hormone 31 (DH31) signaling from the CG17415 receptor. RAMP subtype expression did not alter the pharmacological profile of CG17415 activation. CG17415 antibodies revealed expression within the principal cells of Malpighian tubules, further implicating DH31 as a ligand for this receptor. Immunostaining in the brain revealed an unexpected convergence of two distinct DH signaling pathways. In both the larval and adult brain, most DH31 receptor-expressing neurons produce the neuropeptide corazonin, and also express the CRFR-related receptor CG8422, which is a receptor for the neuropeptide diuretic hormone 44 (DH44). There is extensive convergence of CRF and CGRP signaling within vertebrates and we report a striking parallel in Drosophila involving DH44 (CRF) and DH31 (CGRP). Therefore, it appears that both the molecular details as well as the functional organization of CGRP signaling have been conserved.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A novel diuretic hormone receptor inDrosophila: evidence for conservation of CGRP signaling

Johnson

Shafer

Trigg

et al. 2005

Journal of Experimental Biology

227

142

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“…The CGRP receptor complex comprises calcitonin receptor-like receptor (Crlr; Calcr1a -Zebrafish Information Network), receptor activitymodifying protein 1 (Ramp1) and Rpc9, which facilitates coupling of Gα receptors. When the CGRP receptor complex is activated, CGRPmediated signal is transduced through activating cyclic adenosine monophosphate (cAMP) signaling pathway in mouse and human (Prado et al, 2002;Russo, 2015). However, whether Rpc9 is involved in developmental hematopoiesis during embryogenesis is still unknown.…”

Section: Introductionmentioning

confidence: 99%

RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish

Wei

Zhang

et al. 2016

Development

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Hematopoietic stem and progenitor cells (HSPCs) are capable of self-renewal and replenishing all lineages of blood cells throughout the lifetime and thus critical for tissue homeostasis. However, the mechanism regulating HSPC development is still incompletely understood. Here, we isolate a zebrafish mutant with defective T lymphopoiesis and positional cloning identifies that Rpc9, a component of DNA-directed RNA polymerase III (Pol III) complex, is responsible for the mutant phenotype. Further analysis shows that rpc9-deficiency leads to the impairment of HSPCs and their derivatives in zebrafish embryos. Excessive apoptosis is observed in the caudal hematopoietic tissue (CHT, the equivalent of fetal liver in mammals) of rpc9−/− embryos and the hematopoietic defects in rpc9−/− embryos can be fully rescued by suppression of p53. Thus, our work illustrate that Rpc9, a component of Pol III, plays an important tissue-specific role in HSPC maintenance during zebrafish embryogenesis and that it might be conserved across vertebrates including mammals.

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“…Another possibility could be that the CGRP receptor complex can be affected by the absence of dystrophin. It is known that the CGRP receptor is a member of the G-protein-coupled receptor superfamily, containing a membrane-spanning receptor protein, a receptor activitymodifying protein, and a receptor component protein that couples the receptor to the signal transduction pathway (Prado et al, 2002;Fernandez et al, 2003). In addition, CGRP may become incorporated into the synaptic basal lamina by binding to heparan sulfate proteoglycans (Goodearl et al, 1995;Loeb and Fischbach, 1995).…”

Section: Discussionmentioning

confidence: 99%

Distribution of calcitonin gene‐related peptide at the neuromuscular junction of mdx mice

Marques¹,

Minatel²,

Guimarães³

et al. 2004

Anat. Rec.

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In normal skeletal muscle, the protein dystrophin is associated with plasma membrane glycoproteins and may be involved in the stabilization of the sarcolemma. Mutant mdx mice are markedly deficient in dystrophin and show muscle fiber necrosis followed by regeneration. Changes in the distribution of acetylcholine receptors (AChRs) have been reported at the neuromuscular junction of mdx mice possibly as a result of alterations in the release or response to neural trophic factors. One such factor is calcitonin gene-related peptide (CGRP), which has been implicated in AChR synthesis and function. In this study, we used rhodamine-␣-bungarotoxin and anti-CGRP IgG FITC to study AChR and CGRP distribution at the neuromuscular junction of mdx mice. Using laser scanning fluorescence confocal microscopy, it was possible to see that CGRP-like immunoreactivity had a presynaptic distribution, covering the AChRs. Thirty-four percent of dystrophic junctions were found to be labeled with CGRP compared to 80% of control endplates. Since CGRP-positive and -negative fibers showed similar changes in AChR distribution, it is suggested that CGRP is probably not directly involved in the altered pattern of AChR seen in dystrophin-deficient muscle fibers of mdx mice.

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Receptor component protein (RCP): a member of a multi-protein complex required for G-protein-coupled signal transduction

Cited by 52 publications

References 1 publication

A novel diuretic hormone receptor inDrosophila: evidence for conservation of CGRP signaling

A novel diuretic hormone receptor inDrosophila: evidence for conservation of CGRP signaling

RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish

Distribution of calcitonin gene‐related peptide at the neuromuscular junction of mdx mice

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