Receptor Ligand-Facilitated Gene Transfer: Enhancement of Liposome-Mediated Gene Transfer and Expression by Transferrin

Cheng, Pi Wan

doi:10.1089/hum.1996.7.3-275

Cited by 165 publications

(97 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…19 Enhanced transferrin-mediated gene transfer in the presence of the cationic lipid lipofectin in Hela cells has been reported. 20 Using [ 35 S]-labelled plasmid DNA the authors also showed enhanced intracellular DNA transfer by transferrin in the presence of lipofectin.…”

Section: Discussionmentioning

confidence: 99%

“…19 Hela cells transfected with the ligand transferrin also showed enhanced transfection with the liposome lipofectin. 20 We recently reported first results demonstrating enhancement of integrin-mediated gene delivery by addition of a liposome to melanoma, kidney and endothelial cell lines. 21 In the present report, we extend our study on integrinmediated gene delivery to non-cystic fibrosis and cystic fibrosis human tracheal cells and show that the combination of the [K] 16 RGD peptide with the liposome lipofectamine increases the level of transgene expression about 30-fold (P Ͻ 0.01) above that obtained with the peptide alone.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Liposomes enhance delivery and expression of an RGD-oligolysine gene transfer vector in human tracheal cells

et al. 1998

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Liposomes enhance delivery and expression of an RGD-oligolysine gene transfer vector in human tracheal cells

et al. 1998

View full text Add to dashboard Cite

show abstract

“…Transient transfections were performed as described previously except for the following modification (Cheng, 1996;Seki, Iwakawa, Cheng, & Cheng, 2002). Each well of the 48-well plate was exposed to a transfection solution containing 5μg Maackia amurensis agglutinin (EY Lab, Inc, San Mateo, CA) ligand, 3μg DMRIE-cholesterol liposome (Invitrogen, Carlsbad, CA), and 1 μg pCMVLacZ DNA.…”

Section: Quantitation Of Lacz Expression After Transient Transfectionmentioning

confidence: 99%

Cell type-specific activation of the cytomegalovirus promoter by dimethylsulfoxide and 5-Aza-2'-deoxycytidine

Radhakrishnan¹,

Basma²,

Klinkebiel³

et al. 2008

The International Journal of Biochemistry & Cell Biology

Self Cite

View full text Add to dashboard Cite

The cytomegalovirus promoter is a very potent promoter commonly used for driving the expression of transgenes, though it gradually becomes silenced in stably transfected cells. We examined the methylation status of the cytomegalovirus promoter in two different cell lines and characterized its mechanisms of activation by dimethylsulfoxide and 5-Aza-2′-deoxycytidine. The cytomegalovirus promoter stably transfected into Chinese hamster ovary cells is suppressed by DNA methylation-independent mechanisms, which is different from the rat embryonic cardiomyoblast H9c2-Fluc.3 cells in which the cytomegalovirus promoter is silenced by methylation. Dimethylsulfoxide and 5-Aza-2′-deoxycytidine can activate the cytomegalovirus promoter in both cell types by overlapping mechanisms. Dimethylsulfoxide activates the cytomegalovirus promoter in Chinese hamster ovary cells by promoting histone acetylation and the activation of p38 mitogen-activated protein kinase and nuclear factor κB signaling pathways, while 5-Aza-2′-deoxycytidine increases histone acetylation and activates the nuclear factor κB but not the p38 mitogen-activated protein kinase pathway. In H9c2-Fluc.3 cells, both agents promote demethylation of the cytomegalovirus promoter, and enhance its activity exclusively through activation of the nuclear factor κB pathway and to a lesser extent of the p38 mitogen-activated protein kinase pathway. Our findings suggest that suppression and activation of the cytomegalovirus promoter are cell type-specific. These results may be used for developing strategies to enhance the expression of transgenes and the production of recombinant proteins encoded by transgenes controlled by a cytomegalovirus promoter.

show abstract

“…Notably, transferrin incorporated into liposomes can substantially enhance efficiency of gene transfer mediated by the liposome. 29,30 Several groups, including our own, have used recombinant antibody fragments (Fab and ScFv ) as ligands in the design of targeted nonviral gene transfer vectors. 13,14,19 The bifunctional nonviral vector we previously reported is of human origin, but difficulty in large -scale isolation of the Fab protamine fusion proteins was felt to restrict its further development.…”

Section: Discussionmentioning

confidence: 99%

“…28,35 -37 Our study mainly differs from several previous reports in that a human ScFv was incorporated into the vector systems for target delivery. 13,29,31 The large repertoires of human antibodies that are currently available will allow selection of a broad range of ligands for targeting specific cell populations. 38 -40 Whereas selectivity can be achieved for nonviral vectors formulated in vitro through combinations of DNA, ScFv, lipids, and protamine as demonstrated in this report, several important issues need to be carefully addressed for optimal targeting including the amounts and order of the components added into the mixture as well as the bioactivity and appropriate orientation of the ligands.…”

Section: Discussionmentioning

confidence: 99%

Single-chain antibody-mediated gene delivery into ErbB2-positive human breast cancer cells

Stuckert

Bosch

et al. 2001

Cancer Gene Ther

View full text Add to dashboard Cite

Targeted gene transfer by nonviral vectors can be achieved through incorporation of specific ligand( s ) into the vectors. In this study, the effects of incorporation of an anti -ErbB2 single -chain antibody fragment ( ScFv ) into nonviral vectors for targeted gene delivery were investigated. The ML39 ScFv, selected from a human ScFv phage display library and affinity matured in vitro ( K d =1Â10 À 9 M ), was used as ligand specific for the extracellular domain of the tumor surface protein, ErbB2. Two approaches were taken: ( a ) development of a vector that is composed of a bifunctional fusion protein capable of binding DNA with the ErbB2 -specific ML39 ScFv at its N -terminus and a truncated form of human protamine at its C -terminus, and ( b ) formulation and evaluation of delivery vectors consisting of three independent components including ML39 ScFv, protamine, and cationic lipids. We demonstrate that fusion proteins comprised of the ML39 ScFv and a truncated form of protamine, denoted as ScFv -P -S, can selectively deliver exogenous DNA into ErbB2( + ) cells, with an 8 -to 10 -fold increase in expression levels of the luciferase reporter gene in ErbB2( + ) cells as compared to ErbB2( À ) cells. In addition, vectors formulated by appropriately mixing DNA, ScFv, protamine, and lipids in vitro could even more efficiently deliver the reporter gene into ErbB2( + ) cells with approximately 5 -fold increase in gene expression in ErbB2( + ) cell as compared to ErbB2( À ) cells. Expression and refolding of the ScFv fusion proteins, in addition to determination of optimal conditions for vector development using these approaches, are discussed.

show abstract

Receptor Ligand-Facilitated Gene Transfer: Enhancement of Liposome-Mediated Gene Transfer and Expression by Transferrin

Cited by 165 publications

References 26 publications

Liposomes enhance delivery and expression of an RGD-oligolysine gene transfer vector in human tracheal cells

Liposomes enhance delivery and expression of an RGD-oligolysine gene transfer vector in human tracheal cells

Cell type-specific activation of the cytomegalovirus promoter by dimethylsulfoxide and 5-Aza-2'-deoxycytidine

Single-chain antibody-mediated gene delivery into ErbB2-positive human breast cancer cells

Contact Info

Product

Resources

About