Receptor-like kinase HAESA-like 1 positively regulates seed longevity in Arabidopsis

Chen, Defu; Guo, Hongye; Chen, Shuai; Yue, Q.; Wang, Pei; Chen, Xiwen

doi:10.1007/s00425-022-03942-y

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…The mutant isolation, accelerated aging and germination assays were performed as described previously (Chen et al., 2022). The primers used for genotyping and expression analyses are provided in Table S2.…”

Section: Methodsmentioning

confidence: 99%

Disruption of BG14 results in enhanced callose deposition in developing seeds and decreases seed longevity and seed dormancy in Arabidopsis

et al. 2023

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Seed longevity is an important trait for agriculture and the conservation of genetic resources. b-1,3-Glucanases were first recognized as pathogenesis-related proteins involved in plant defense, but their roles in seeds are largely unknown. Here, we report a glycosylphosphatidylinositol-anchored b-1,3-glucanase, BG14, that degrades callose in seed embryos and functions in seed longevity and dormancy in Arabidopsis. The loss of function of BG14 significantly decreased seed longevity, whereas functional reversion (RE) and overexpression (OE) lines reversed and increased the impaired phenotype, respectively. The loss of function of BG14 enhanced callose deposition in the embryos of mature seeds, confirmed by quantitative determination and the decreased callose degrading ability in bg14. The drop-and-see (DANS) assay revealed that the fluorescence signal in bg14 was significantly lower than that observed in the other three genotypes. BG14 is located on the periphery of the cell wall and can completely merge with callose at the plasmodesmata of epidermal cells. BG14 was highly expressed in developing seeds and was induced by aging and abscisic acid (ABA). The loss of function of BG14 led to a variety of phenotypes related to ABA, including reduced seed dormancy and reduced responses to treatment with ABA or pacolblltrazol, whereas OE lines showed the opposite phenotype. The reduced ABA response is because of the decreased level of ABA and the lowered expression of ABA synthesis genes in bg14. Taken together, this study demonstrated that BG14 is a bona fide BG that mediates callose degradation in the plasmodesmata of embryo cells, transcriptionally influences ABA synthesis genes in developing seeds, and positively affects seed longevity and dormancy in Arabidopsis.

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Section: Methodsmentioning

confidence: 99%

Disruption of BG14 results in enhanced callose deposition in developing seeds and decreases seed longevity and seed dormancy in Arabidopsis

et al. 2023

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“…Intracellular kinase structures are activated when the extracellular LRR domain of LRR-RLKs binds to ligands, which leads to the phosphorylation of downstream proteins and the activation of downstream signaling pathways, then regulates various physiological and biochemical processes in plants [8,9]. Thus far, LRR-RLKs have been reported to play an important role in plant growth and development as well as plant responses to abiotic and biotic stresses, including pathogens [10][11][12][13][14]. The flagellin-derived peptide flg22, for instance, induces the development of a monomeric heterodimer of LRR-RLK BAK1 and FLS2 in Arabidopsis, followed by an immune system response [15,16].…”

Section: Introductionmentioning

confidence: 99%

Knocking Out OsRLK7-1 Impairs Rice Growth and Development but Enhances Its Resistance to Planthoppers

Han,

Shen,

Gao

et al. 2023

IJMS

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Leucine-rich repeat receptor-like kinases (LRR-RLKs) are an important subfamily of receptor-like kinases (RLKs) in plants that play key roles in sensing different biotic and abiotic stress. However, the role of LRR-RLKs in herbivore-induced plant defense remains largely elusive. Here, we found that the expression of a rice gene, OsRLK7-1, was induced by mechanical wounding, but was slightly suppressed by the infestation of gravid females of brown planthopper (BPH, Nilaparvata lugens) or white-backed planthopper (WBPH, Sogatella furcifera). Through targeted disruption of OsRLK7-1 (resulting in the ko-rlk lines), we observed an augmentation in transcript levels of BPH-induced OsMPK3, OsWRKY30, OsWRKY33, and OsWRKY45, alongside heightened levels of planthopper-induced jasmonic acid, JA-isoleucine, and abscisic acid in plant tissues. These dynamic changes further facilitated the biosynthesis of multiple phenolamides within the rice plants, culminating in an enhanced resistance to planthopper infestations under both lab and field conditions. In addition, knocking out OsRLK7-1 impaired plant growth and reproduction. These results suggest that OsRLK7-1 plays an important role in regulating rice growth, development, and rice-planthopper interactions.

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“…The complexity of the RLK superfamily may reflect the intricate coordination of plant responses to external signals during plant development and interactions with the biotic and abiotic environment. Accordingly, several RLKs have been functionally characterized in development, environmental stresses, and plant defenses (for more details, see references [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]).…”

Section: Introductionmentioning

confidence: 99%

RLPredictiOme, a Machine Learning-Derived Method for High-Throughput Prediction of Plant Receptor-like Proteins, Reveals Novel Classes of Transmembrane Receptors

Silva¹,

Ferreira

Carvalho

et al. 2022

IJMS

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Cell surface receptors play essential roles in perceiving and processing external and internal signals at the cell surface of plants and animals. The receptor-like protein kinases (RLK) and receptor-like proteins (RLPs), two major classes of proteins with membrane receptor configuration, play a crucial role in plant development and disease defense. Although RLPs and RLKs share a similar single-pass transmembrane configuration, RLPs harbor short divergent C-terminal regions instead of the conserved kinase domain of RLKs. This RLP receptor structural design precludes sequence comparison algorithms from being used for high-throughput predictions of the RLP family in plant genomes, as has been extensively performed for RLK superfamily predictions. Here, we developed the RLPredictiOme, implemented with machine learning models in combination with Bayesian inference, capable of predicting RLP subfamilies in plant genomes. The ML models were simultaneously trained using six types of features, along with three stages to distinguish RLPs from non-RLPs (NRLPs), RLPs from RLKs, and classify new subfamilies of RLPs in plants. The ML models achieved high accuracy, precision, sensitivity, and specificity for predicting RLPs with relatively high probability ranging from 0.79 to 0.99. The prediction of the method was assessed with three datasets, two of which contained leucine-rich repeats (LRR)-RLPs from Arabidopsis and rice, and the last one consisted of the complete set of previously described Arabidopsis RLPs. In these validation tests, more than 90% of known RLPs were correctly predicted via RLPredictiOme. In addition to predicting previously characterized RLPs, RLPredictiOme uncovered new RLP subfamilies in the Arabidopsis genome. These include probable lipid transfer (PLT)-RLP, plastocyanin-like-RLP, ring finger-RLP, glycosyl-hydrolase-RLP, and glycerophosphoryldiester phosphodiesterase (GDPD, GDPDL)-RLP subfamilies, yet to be characterized. Compared to the only Arabidopsis GDPDL-RLK, molecular evolution studies confirmed that the ectodomain of GDPDL-RLPs might have undergone a purifying selection with a predominance of synonymous substitutions. Expression analyses revealed that predicted GDPGL-RLPs display a basal expression level and respond to developmental and biotic signals. The results of these biological assays indicate that these subfamily members have maintained functional domains during evolution and may play relevant roles in development and plant defense. Therefore, RLPredictiOme provides a framework for genome-wide surveys of the RLP superfamily as a foundation to rationalize functional studies of surface receptors and their relationships with different biological processes.

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Receptor-like kinase HAESA-like 1 positively regulates seed longevity in Arabidopsis

Cited by 6 publications

References 42 publications

Disruption of BG14 results in enhanced callose deposition in developing seeds and decreases seed longevity and seed dormancy in Arabidopsis

Disruption of BG14 results in enhanced callose deposition in developing seeds and decreases seed longevity and seed dormancy in Arabidopsis

Knocking Out OsRLK7-1 Impairs Rice Growth and Development but Enhances Its Resistance to Planthoppers

RLPredictiOme, a Machine Learning-Derived Method for High-Throughput Prediction of Plant Receptor-like Proteins, Reveals Novel Classes of Transmembrane Receptors

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