Receptor-Mediated Active Adhesion to the Substratum Is Required for Neurite Outgrowth

Turner, David C.; Flier, Leonard A.

doi:10.1159/000111908

Cited by 16 publications

(7 citation statements)

References 22 publications

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“…In extreme cases the distribution of the cells over the culture surface was also altered, with a higher fraction of clustered neurons. An effect of substrate on the survival and growth of neurons has also been documented by other authors (Reichardt et al, 1989;Sephel et al, 1989;Turner and Flier, 1989;Lieth et al, 1990;Banker and Goslin, 1991;Lein et al, 1992;Doherty et al, 1995). There is a general consensus that during the first week in vitro the nature of the substrate affects the growth and survival of neurons.…”

Section: Early Neuronal Differentiation: Neuritic Developmentmentioning

confidence: 53%

Neuritic differentiation and synaptogenesis in serum-free neuronal cultures of the rat cerebral cortex

1997

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To better understand the dynamics of the cellular processes involved in early neocortical development, we studied the neuritic differentiation and synaptogenesis of dispersed neurons grown in serum-free cultures under a wide variety of culture conditions. Microtubule-associated protein (MAP2), phosphorylated neurofilament (SMI 31) and synaptophysin immunocytochemistry was complemented with time-lapse studies. During the first week in vitro dissociated cortical neurons developed from roundish cells without processes to neurons with axons and differentiated dendrites, going through five distinct phases. The sequence of these phases was unaltered in a wide range of culturing methods, but the timing of the steps varied among cultures started with different cell densities. Synaptic terminals were first observed after 3-4 days in vitro, coincident with the beginning of dendritic differentiation. Synaptogenesis progressed at least until the end of the third week in vitro, despite a decline in cell density during the second week in vitro. The process of cellular differentiation of cerebral cortical neurons in vitro resembled the development of these cells in the intact tissue, suggesting that organized cell migration is not a prerequisite for the differentiation of single cortical neurons.

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Section: Early Neuronal Differentiation: Neuritic Developmentmentioning

confidence: 53%

Neuritic differentiation and synaptogenesis in serum-free neuronal cultures of the rat cerebral cortex

1997

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“…Receptor-specific interactions or "active" adhesion is believed to be a necessary condition for proper cell differentiation. 34 The ability to effectively manage these interactions has been previously accomplished by geometrically patterning extracellular matrix pathways that are permissive towards a particular cellular process, for example laminin for neurite outgrowth. However, this is not sufficient to control the guidance of a differentiating neuronal cell.…”

Section: Discussionmentioning

confidence: 99%

Spatial control of neuronal cell attachment and differentiation on covalently patterned laminin oligopeptide substrates

Ranieri

Bellamkonda

Bekos

et al. 1994

Intl J of Devlp Neuroscience

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The spatial control of neuronal cell attachment and differentiation via specific receptor mediated interactions, may provide an effective means for the in vitro reconstruction of neuronal cell architecture. In this study, receptor-specific oligopeptide sequences derived from the extracellular matrix (ECM) molecule laminin, a potent neural cell attachment and differentiation promoter were covalently bound on fluorinated ethylene propylene (FEP) films. The degree of receptor-specific cell attachment and the ability to spatially control neurite outgrowth by covalently patterning the oligopeptide sequences on the FEP film surface were assessed. FEP films were first chemically activated with a Radio Frequency Glow Discharge (RFGD) process that covalently replaces the surface fluorine atoms with reactive hydroxyl groups. Oligopeptides containing the YIGSR sequence from the B1 chain of laminin and the water soluble oligopeptide containing the IKVAV sequence (CSRARKQAASIKVAVSADR) from the A chain were covalently bound to the hydroxylated FEP films. Electron Spectroscopy for Chemical Analysis (ESCA) verified the covalent attachment of the oligopeptides to the material surface. The degree of receptor mediated NG108-15 cell attachment on immobilized CDPGYIGSR films was determined using competitive binding media. A 78% reduction in cell attachment was observed on films containing CDPGYIGSR in the cell plating medium. Only a 23% reduction in cell attachment was noted on films plated in medium containing a mock CDPGYIGSK sequence. FEP films immobilized with the IKVAV oligopeptide sequence were shown to mediate PC12 cell attachment and a competitive binding medium also significantly attenuated cell attachment on the immobilized films. The spatial patterning of these oligopeptide sequences to the FEP surface was shown to localize cell attachment and neurite extension on the patterned pathways. The surrounding unmodified FEP surface was inhibitory in serum containing medium and prevented cellular interactions outside the oligopeptide modifications. The spatial immobilization of laminin oligopeptides on FEP films provides a means to organize the attachment and differentiation of neuronal cells in a receptor-specific manner.

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“…Both soluble and substrate-bound factors can either attract or repel growth cones (Fawcett and Asher 1999;Fournier and Strittmatter 2001;TessierLavigne and Goodman 1996). In response to these factors, growth cones orient and turn as well as stop growing prior to synapse formation (Turner and Flier 1989). Molecules involved in axon growth and guidance are phylogenetically conserved from the level of lower invertebrates to mammals and, therefore, information derived from invertebrates is relevant to other species (McKay et al 1999;Tessier-Lavigne and Goodman 1996).…”

Section: Introductionmentioning

confidence: 99%

Arterial cells and CNS sheath cells from Aplysia californica produce factors that enhance neurite outgrowth in co-cultured neurons

Montgomery

Messner

Kirk

2002

Invertebrate Neuroscience

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Substrate-bound and soluble factors regulate neurite outgrowth and synapse formation during development, regeneration, and learning and memory. We report that sheath cells from CNS connectives and arterial cells from the anterior aorta of the sea slug, Aplysia californica, enhance neurite outgrowth from co-cultured Aplysia neurons. Sheath and arterial cell cultures contain several cell types, including fibrocytes, myocytes, and amoebocytes. When compared to controls (neurons with defined growth medium alone), the percentage of neurons with growth and the average neurite lengths are significantly enhanced by sheath and arterial cells at 48 h after plating of the neurons; these parameters are comparable to those of neurons cultured in medium containing hemolymph. Our results indicate that sheath cells produce substrate-bound factor(s) and arterial cells produce diffusible factor(s) that promote growth. These growth factors likely promote neuron survival and neurite outgrowth during neural plasticity exhibited in the adult CNS.

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Receptor-Mediated Active Adhesion to the Substratum Is Required for Neurite Outgrowth

Cited by 16 publications

References 22 publications

Neuritic differentiation and synaptogenesis in serum-free neuronal cultures of the rat cerebral cortex

Neuritic differentiation and synaptogenesis in serum-free neuronal cultures of the rat cerebral cortex

Spatial control of neuronal cell attachment and differentiation on covalently patterned laminin oligopeptide substrates

Arterial cells and CNS sheath cells from Aplysia californica produce factors that enhance neurite outgrowth in co-cultured neurons

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