Receptor-Mediated and Fluid-Phase Transcytosis of Horseradish Peroxidase across Rat Hepatocytes

Ellinger, Isabella; Fuchs, Renate

doi:10.1155/2010/850320

Cited by 9 publications

(9 citation statements)

References 52 publications

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“…Dextran labeled with FITC or other fluorophores is generally applied as a fluid phase marker, especially to determine the involvement of macropinocytosis in the internalization of cargo . Although we do not consider that dextran in a complex with protamine, DNA and SLN functions as a fluid phase marker, recent published data demonstrate that the dextran alters the properties of protamine‐DNA‐SLN complexes with respect to erythrocyte agglutination .…”

Section: Discussionmentioning

confidence: 91%

Role of endocytic uptake in transfection efficiency of solid lipid nanoparticles‐based nonviral vectors

Garibay

Aspiazu

Rodríguez-Gascón

et al. 2013

The Journal of Gene Medicine

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Section: Discussionmentioning

confidence: 91%

Role of endocytic uptake in transfection efficiency of solid lipid nanoparticles‐based nonviral vectors

Garibay

Aspiazu

Rodríguez-Gascón

et al. 2013

The Journal of Gene Medicine

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“…Mannose receptors have been reported to be constitutively expressed by liver macrophages, endothelial cells and hepatocytes (Kempka and Kolb-Bachofen, 1988; Ellinger and Fuchs, 2010). However, while mannose receptor expression is upregulated in liver macrophages and endothelial cells by anti-inflammatory cytokines, this is not observed in hepatocytes (Liu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Regulation of alternative macrophage activation in the liver following acetaminophen intoxication by stem cell-derived tyrosine kinase

Gardner

Hankey

Mishin

et al. 2012

Toxicology and Applied Pharmacology

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Stem cell-derived tyrosine kinase (STK) is a transmembrane receptor reported to play a role in macrophage switching from a classically activated/proinflammatory phenotype to an alternatively activated/wound repair phenotype. In the present studies, STK−/− mice were used to assess the role of STK in acetaminophen-induced hepatotoxicity as evidence suggests that the pathogenic process involves both of these macrophage subpopulations. In wild type mice, centrilobular hepatic necrosis and increases in serum transaminase levels were observed within 6 hr of acetaminophen administration (300 mg/kg, i.p.). Loss of STK resulted in a significant increase in sensitivity of mice to the hepatotoxic effects of acetaminophen and increased mortality, effects independent of its metabolism. This was associated with reduced levels of hepatic glutathione, rapid upregulation of inducible nitric oxide synthase, and prolonged induction of heme oxygenase-1, suggesting excessive oxidative stress in STK−/− mice. F4/80, a marker of mature macrophages, was highly expressed on subpopulations of Kupffer cells in livers of wild type, but not STK −/− mice. Whereas F4/80+ macrophages rapidly declined in the livers of wild type mice following acetaminophen intoxication, they increased in STK−/− mice. In wild type mice hepatic expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-12, products of classically activated macrophages, increased after acetaminophen administration. Monocyte chemotactic protein-1 (MCP-1) and its receptor, CCR2, as well as IL-10, mediators involved in recruiting and activating anti-inflammatory/wound repair macrophages, also increased in wild type mice after acetaminophen. Loss of STK blunted the effects of acetaminophen on expression of TNFα, IL-1β, IL-12, MCP-1 and CCR2, while expression of IL-10 increased. Hepatic expression of CX3CL1, and its receptor, CX3CR1 also increased in STK−/− mice treated with acetaminophen. These data demonstrate that STK plays a role in regulating macrophage recruitment and activation in the liver following acetaminophen administration, and in hepatotoxicity.

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“…These findings differed from those of other studies in which a large amount of horseradish peroxidase ( MW = 40,000) diffused into the parenchyma in the OVLT (Herde et al, 2011 ) and ME (Broadwell et al, 1983 ; Rodríguez et al, 2010 ). Horseradish peroxidase is known to be incorporated by mannose receptor-mediated transcellular and clathrin-mediated transcytosis routes (Ellinger and Fuchs, 2010 ). In the medial basal hypothalamus, wheat germ agglutinin lectin ( MW = 38,000) was shown to be taken up by tanycytes in the arcuate nucleus (Arc) via cellular internalization (Peruzzo et al, 2004 ; Morita et al, 2013a ).…”

Section: Size-selective Vascular Permeabilitymentioning

confidence: 99%

New aspects in fenestrated capillary and tissue dynamics in the sensory circumventricular organs of adult brains

2015

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The blood–brain barrier (BBB) generally consists of endothelial tight junction barriers that prevent the free entry of blood-derived substances, thereby maintaining the extracellular environment of the brain. However, the circumventricular organs (CVOs), which are located along the midlines of the brain ventricles, lack these endothelial barriers and have fenestrated capillaries; therefore, they have a number of essential functions, including the transduction of information between the blood circulation and brain. Previous studies have demonstrated the extensive contribution of the CVOs to body fluid and thermal homeostasis, energy balance, the chemoreception of blood-derived substances, and neuroinflammation. In this review, recent advances have been discussed in fenestrated capillary characterization and dynamic tissue reconstruction accompanied by angiogenesis and neurogliogenesis in the sensory CVOs of adult brains. The sensory CVOs, including the organum vasculosum of the lamina terminalis (OVLT), subfornical organ (SFO), and area postrema (AP), have size-selective and heterogeneous vascular permeabilities. Astrocyte-/tanycyte-like neural stem cells (NSCs) sense blood- and cerebrospinal fluid-derived information through the transient receptor potential vanilloid 1, a mechanical/osmotic receptor, Toll-like receptor 4, a lipopolysaccharide receptor, and Nax, a Na-sensing Na channel. They also express tight junction proteins and densely and tightly surround mature neurons to protect them from blood-derived neurotoxic substances, indicating that the NSCs of the CVOs perform BBB functions while maintaining the capacity to differentiate into new neurons and glial cells. In addition to neurogliogenesis, the density of fenestrated capillaries is regulated by angiogenesis, which is accompanied by the active proliferation and sprouting of endothelial cells. Vascular endothelial growth factor (VEGF) signaling may be involved in angiogenesis and neurogliogenesis, both of which affect vascular permeability. Thus, recent findings advocate novel concepts for the CVOs, which have the dynamic features of vascular and parenchymal tissues.

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Receptor-Mediated and Fluid-Phase Transcytosis of Horseradish Peroxidase across Rat Hepatocytes

Cited by 9 publications

References 52 publications

Role of endocytic uptake in transfection efficiency of solid lipid nanoparticles‐based nonviral vectors

Role of endocytic uptake in transfection efficiency of solid lipid nanoparticles‐based nonviral vectors

Regulation of alternative macrophage activation in the liver following acetaminophen intoxication by stem cell-derived tyrosine kinase

New aspects in fenestrated capillary and tissue dynamics in the sensory circumventricular organs of adult brains

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