Receptor mediated endocytosis and intracellular fate of interleukin 1

Solari, Roberto; Smithers, Nick; Kennard, N; Ray, Keith P.; Grenfell, S. J.

doi:10.1016/0006-2952(94)90441-3

Cited by 30 publications

(14 citation statements)

References 28 publications

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“…Use of these treatments was selected in our study because they are effective (see our data) and noninvasive methods to inhibit endocytosis, whereas experiments using metabolic poisons may be limited in their interpretation due to nonspecific effects unrelated to endocytosis. In addition, lowering the temperature below 17°C or incubation in hypertonic medium have been reported to severely reduce the cellular internalization of TNF-␣ (30,47,48) and IL-1␤ (31,49). In contrast, we demonstrate that under these conditions TNF-␣ and IL-1␤ activate SPM hydrolysis as well in two different cell types.…”

Section: Low Temperatures and Hypertonic Media Inhibit Receptormediatmentioning

confidence: 48%

“…At low temperature, TNF can also stimulate the phosphorylation of a p26 cytosolic protein (33). The role of internalization in IL-1␤ signaling also remains unclear (49). A subclone of EL4 cells has been described that binds but does not respond to IL-1 possibly due to its inability to internalize the cytokine (52).…”

Section: Low Temperatures and Hypertonic Media Inhibit Receptormediatmentioning

confidence: 99%

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Low Temperatures and Hypertonicity Do Not Block Cytokine-induced Stimulation of the Sphingomyelin Pathway but Inhibit Nuclear Factor-κB Activation

Andrieu

Salvayre

Jaffrézou

et al. 1995

Journal of Biological Chemistry

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In order to better understand the significance of tumor necrosis factor-␣ (TNF-␣) and interleukin-1␤ (IL-1␤)-receptor internalization in the sphingomyelin pathway signal transduction, we investigated receptor signaling under conditions in which receptor internalization is blocked. We demonstrate that human recombinant TNF-␣ and IL-1␤ both induced sphingomyelin and phosphatidylcholine hydrolysis at either 4, 14, or 37°C in human skin fibroblasts and U937 monocytic cells. Cytokine-induced sphingomyelin degradation also occurred when endocytosis was inhibited by incubating the cells in hypertonic medium. While internalization was not required for the production of ceramide, activation of the transcription factor NF-B was strongly reduced when cells were stimulated with TNF at low temperature or in hypertonic medium. Under these conditions, activation of NF-B by the cell-permeant C 2 -ceramide (N-acetylsphingosine), by exogenous sphingomyelinase or by phorbol myristate acetate was also inhibited. These results suggest that low temperature and hypertonicity, two inhibitors of receptor internalization: (i) do not affect the TNF-␣-or IL-1␤-induced sphingomyelin hydrolysis, but (ii) do inhibit a step distal to ceramide of the intracellular signaling pathway leading to NF-B activation.

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Section: Low Temperatures and Hypertonic Media Inhibit Receptormediatmentioning

confidence: 48%

Section: Low Temperatures and Hypertonic Media Inhibit Receptormediatmentioning

confidence: 99%

Low Temperatures and Hypertonicity Do Not Block Cytokine-induced Stimulation of the Sphingomyelin Pathway but Inhibit Nuclear Factor-κB Activation

Andrieu

Salvayre

Jaffrézou

et al. 1995

Journal of Biological Chemistry

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“…The downregulation of cytokine receptors through internalisation can help to decrease the beta cell toxicity of proinflammatory cytokines. Internalisation of the IL-1β receptor has been described in different cell types, where the internalised receptor accumulated in the cytosol and could be recycled to the plasma membrane [70,71,72]. Receptor-mediated endocytosis was also shown for IFN-γ, but in contrast to the recycling of the IL-1β receptor, a lysosomal ligandreceptor degradation occurred after internalisation [73].…”

Section: Discussionmentioning

confidence: 99%

Cytokines activate genes of the endocytotic pathway in insulin-producing RINm5F cells

et al. 2004

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Aims/hypothesis. Cytokines are important humoral mediators of beta cell destruction in autoimmune diabetes. The aim of this study was to identify novel cytokine-induced genes in insulin-producing RINm5F cells, which may contribute to beta cell death or survival. Methods. A global gene expression profile in cytokine-exposed insulin-producing RINm5F cells was achieved by automated restriction fragment differential display PCR. The expression of selected candidate genes was confirmed by real-time RT-PCR analysis. Results. Exposure of RINm5F cells to IL-1β or to a cytokine mixture (IL-1β, TNF-α, IFN-γ) for 6 h resulted in the differential expression of a functional gene cluster. Apart from the well-known up-regulation of the cytokine-responsive genes iNOS, NF-κB, MnSOD and Hsp70, several genes that belong to the functional cluster of the endocytotic pathway were identified. These endocytotic genes comprised: clathrin, megalin, synaptotagmin and calcineurin, which were up-regulated by IL-1β or the cytokine mixture.In contrast, the expression of the calcineurin inhibitor CAIN and of the GDP/GTP exchange protein Rab3 was down-regulated by cytokines. Other up-regulated cytokine-responsive genes were: agrin, murine adherent macrophage protein mRNA (MAMA) and transport-associated protein (TAP1/MTP), whereas the plasma membrane calcium ATPase (PMCA) 2 and PMCA 3 genes were down-regulated by cytokines. Conclusions/interpretation. Our results indicate that genes of the endocytotic pathway are regulated by pro-inflammatory cytokines. This might affect the density of cytokine receptors at the beta cell surface and concomitantly the sensitivity of the cells to cytokine toxicity. A better understanding of the functional cross-talk between endocytotic and cytokine signalling pathways could further the development of novel strategies to protect pancreatic beta cells against toxic effects of pro-inflammatory cytokines.

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“…Cytokine Receptor Internalization-Cytokine receptor internalization was carried out as described (42). In short, HCT-116 cells were first washed in PBS, trypsinized, and counted.…”

Section: Sequencementioning

confidence: 99%

Pyropheophorbide-a Methyl Ester-mediated Photosensitization Activates Transcription Factor NF-κB through the Interleukin-1 Receptor-dependent Signaling Pathway

Matroule¹,

Bonizzi²,

Morlière³

et al. 1999

Journal of Biological Chemistry

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Pyropheophorbide-a methyl ester (PPME) is a second generation of photosensitizers used in photodynamic therapy. We demonstrated that PPME photosensitization activated NF-B transcription factor in colon cancer cells. Unexpectedly, this activation occurred in two separate waves, i.e. a rapid and transient one and a second slower but sustained phase. The former was due to photosensitization by PPME localized in the cytoplasmic membrane which triggered interleukin-1 receptor internalization and the transduction pathways controlled by the interleukin-1 type I receptor. Indeed, TRAF6 dominant negative mutant abolished NF-B activation by PPME photosensitization, and TRAF2 dominant negative mutant was without any effect, and overexpression of IB kinases increased gene transcription controlled by NF-B. Oxidative stress was not likely involved in the activation. On the other hand, the slower and sustained wave could be the product of the release of ceramide through activation of the acidic sphingomyelinase. PPME localization within the lysosomal membrane could explain why ceramide acted as second messenger in NF-B activation by PPME photosensitization. These data will allow a better understanding of the molecular basis of tumor eradication by photodynamic therapy, in particular the importance of the host cell response in the treatment.

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Receptor mediated endocytosis and intracellular fate of interleukin 1

Cited by 30 publications

References 28 publications

Low Temperatures and Hypertonicity Do Not Block Cytokine-induced Stimulation of the Sphingomyelin Pathway but Inhibit Nuclear Factor-κB Activation

Low Temperatures and Hypertonicity Do Not Block Cytokine-induced Stimulation of the Sphingomyelin Pathway but Inhibit Nuclear Factor-κB Activation

Cytokines activate genes of the endocytotic pathway in insulin-producing RINm5F cells

Pyropheophorbide-a Methyl Ester-mediated Photosensitization Activates Transcription Factor NF-κB through the Interleukin-1 Receptor-dependent Signaling Pathway

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