2008
DOI: 10.1016/j.humimm.2008.07.010
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Receptor-mediated endocytosis of particles by peripheral dendritic cells

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Cited by 18 publications
(17 citation statements)
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“…According to earlier reports, the zeolite particles used had an average hydrodynamic diameter of 600 nm [20] and previous reports have shown that zeolite particles associated with cells after washing are almost completely localized intra-cellular with only a minor fraction adsorbed on the cell surface [15,20,21]. Consequently, particles firmly associated with cells after incubation and washing, are demonstrability phagocytosed by the cells in question.…”
Section: Discussionmentioning
confidence: 92%
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“…According to earlier reports, the zeolite particles used had an average hydrodynamic diameter of 600 nm [20] and previous reports have shown that zeolite particles associated with cells after washing are almost completely localized intra-cellular with only a minor fraction adsorbed on the cell surface [15,20,21]. Consequently, particles firmly associated with cells after incubation and washing, are demonstrability phagocytosed by the cells in question.…”
Section: Discussionmentioning
confidence: 92%
“…Coating of zeolites were done as earlier described [15,20,21] and briefly volumes of 1Âg supernatants corresponding to 1 mg zeolite particles were centrifuged for 5 min at 13,000Âg and the collected zeolites were suspended and incubated in 0.5 ml of Tris-buffer containing Alexa-OA or DQ-OA on a rocking table for 1 h using a final concentrations of 0.2 mg/ml Alexa-OA or DQ-OA. The zeolites were collected and washed twice with 1 ml PBS.…”
Section: Coating Zeolitesmentioning
confidence: 99%
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“…Another question refers to the efficient uptake of the nanoparticles. The particles enter the cells by endocytosis, a process which is influenced by the size of the nanoparticles 21 , the cell type and the differentiation status 22 and the cellular capability to perform phago-and macropinocytosis 23 . The optimum concentration to obtain a satisfying fluorescence signal has to be tested for each individual application or cell line.…”
Section: Discussionmentioning
confidence: 99%