Receptor signaling and the cell biology of synaptic transmission

Yu, Jiang-Zhou; Rasenick, Mark M.

doi:10.1016/b978-0-444-52002-9.00002-4

Cited by 6 publications

(4 citation statements)

References 134 publications

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“…We focused on genes related to the signaling pathway of the brainderived neurotrophic factor (BDNF) through its tropomyosin kinase B receptor (TrkB). Thus, we also measured the expression of the transcription factor cAMP response element-binding (CREB), which not only belongs to the BDNF/TrkB pathway but also regulates the expression of hundreds of genes, including BDNF (Carlezon et al, 2005;Yu and Rasenick, 2012). The expression of the Rho GTPase activating protein 32 (ARHGAP32, also known as p250GAP) was also analyzed as it is involved in structural plasticity by regulating the molecular changes associated with cytoskeleton remodeling (Nakazawa et al, 2003;Marler et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Neurobehavioral Effects of Restricted and Unpredictable Environmental Enrichment in Rats

2020

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To study how motivational factors modulate experience-dependent neurobehavioral plasticity, we modify a protocol of environmental enrichment (EE) in rats. We assumed that the benefits derived from EE might vary according to the level of incentive salience attributed to it. To enhance the rewarding properties of EE, access to the EE cage varied randomly from 2 to 48 h for 30 days (REE). The REE group was enriched only 50% of the time and was compared to standard housing and continuous EE (CEE) groups. As behavioral readout, we analyzed the spontaneous activity and the ultrasonic vocalizations (USVs) within the EE cage weekly, and in the open field test at the end of the experiment. In the cage, REE increased the utilization of materials, physical activity, and the rate of appetitive USVs. In the OF, the CEE-induced enhancements in novelty habituation and social signaling were equaled by the REE. At the neural level, we measured the expression of genes related to neural plasticity and epigenetic regulations in different brain regions. In the dorsal striatum and hippocampus, REE upregulated the expression of the brainderived neurotrophic factor, its tropomyosin kinase B receptor, and the DNA methyltransferase 3A. Altogether, our results suggest that the higher activity within the cage and the augmented incentive motivation provoked by the REE boosted its neurobehavioral effects equaling or surpassing those observed in the CEE condition. As constant exposures to treatments or stimulating environments are virtually impossible for humans, restricted EE protocols would have greater translational value than traditional ones.

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Section: Introductionmentioning

confidence: 99%

Neurobehavioral Effects of Restricted and Unpredictable Environmental Enrichment in Rats

2020

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“…Lastly, the largely increased levels of serum palmitic acid, accompanied by the elevations in glycerol 1-palmitic acid, are indicative of a metabolic flux towards phospholipid synthesis, in particular, the glycerophospholipids (Carta et al, 2017), in the PKCδ knock-out model. Considering the functionality of these phospholipids is for regulating membrane permeability (Rajasekaran et al, 2011;Yu & Rasenick, 2012), fat absorption from the intestine (Murota, 2020), elevated mitochondrial function (Hishikawa et al, 2014), cholesterol transport and accumulation in the cells (Blanco & Blanco, 2017;Carta et al, 2017;Castro-Gómez et al, 2015), and preventing fat accumulation in the liver (Carta et al, 2017;Wang et al, 2013), supports the aforementioned observations.…”

Section: Discussionmentioning

confidence: 58%

The metabolomics of a protein kinase C delta (PKCδ) knock-out mouse model

et al. 2022

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Introduction PKCδ is ubiquitously expressed in mammalian cells and its dysregulation plays a key role in the onset of several incurable diseases and metabolic disorders. However, much remains unknown about the metabolic pathways and disturbances induced by PKC deficiency, as well as the metabolic mechanisms involved. Objectives This study aims to use metabolomics to further characterize the function of PKC from a metabolomics standpoint, by comparing the full serum metabolic profiles of PKC deficient mice to those of wild-type mice. Methods The serum metabolomes of PKCδ knock-out mice were compared to that of a wild-type strain using a GCxGC-TOFMS metabolomics research approach and various univariate and multivariate statistical analyses. Results Thirty-seven serum metabolite markers best describing the difference between PKCδ knock-out and wild-type mice were identified based on a PCA power value > 0.9, a t-test p-value < 0.05, or an effect size > 1. XERp prediction was also done to accurately select the metabolite markers within the 2 sample groups. Of the metabolite markers identified, 78.4% (29/37) were elevated and 48.65% of these markers were fatty acids (18/37). It is clear that a total loss of PKCδ functionality results in an inhibition of glycolysis, the TCA cycle, and steroid synthesis, accompanied by upregulation of the pentose phosphate pathway, fatty acids oxidation, cholesterol transport/storage, single carbon and sulphur-containing amino acid synthesis, branched-chain amino acids (BCAA), ketogenesis, and an increased cell signalling via N-acetylglucosamine. ConclusionThe charaterization of the dysregulated serum metabolites in this study, may represent an additional tool for the early detection and screening of PKCδ-deficiencies or abnormalities.

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“…Activation of IP3Rs involves Ca +2 gating and a coagonist‐Inositol‐1,3,5‐phosphate (IP3) (Demuro & Parker, 2013). Phospholipase (PLC) mediated production of IP3 from phosphatidylinositol 4,5‐bisphosphate (PIP2) triggers Ca +2 release (Berridge, 2016; Yu & Rasenick, 2012). Calcium uptake by ER is facilitated through SOCE.…”

Section: Regulation Of Calcium Homeostasis Under Normal Physiological...mentioning

confidence: 99%

“…F I G U R E 2 Role of ER in calcium regulation. (Berridge, 2016;Yu & Rasenick, 2012). Calcium uptake by ER is facilitated through SOCE.…”

Section: Calcium Regulation and Ermentioning

confidence: 99%

Role of calcium dysregulation in Alzheimer's disease and its therapeutic implications

Joshi

Khambete

et al. 2022

Chem Biol Drug Des

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Receptor signaling and the cell biology of synaptic transmission

Cited by 6 publications

References 134 publications

Neurobehavioral Effects of Restricted and Unpredictable Environmental Enrichment in Rats

Neurobehavioral Effects of Restricted and Unpredictable Environmental Enrichment in Rats

The metabolomics of a protein kinase C delta (PKCδ) knock-out mouse model

Role of calcium dysregulation in Alzheimer's disease and its therapeutic implications

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