Receptor studies on isolated astroglial cell fractions prepared with and without trypsin

Henn, Fritz A.; Deering, James; Anderson, David G.

doi:10.1007/bf00964234

Cited by 26 publications

(6 citation statements)

References 4 publications

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“…Previous authors have also used bulk-isolated cells for pharmacological studies of astrocytes (Henn et al, 1980). Those analyses however, were of all cellular elements contained in the cell fractions and could not directly assess the contribution of non-astrocytic elements to the results.…”

Section: Discussionmentioning

confidence: 98%

“…The method of bulk-isolation has been previously employed for the study of astrocytes by other groups (Chatterjee and Sarkar, 1984;Connor and Berkowitz, 1985;Farooq and Norton, 1978;Henn et al, 1980). Previous authors have also used bulk-isolated cells for pharmacological studies of astrocytes (Henn et al, 1980). Those analyses however, were of all cellular elements contained in the cell fractions and could not directly assess the contribution of non-astrocytic elements to the results.…”

Section: Discussionmentioning

confidence: 99%

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Expression of beta‐adrenergic receptors by astrocytes isolated from adult rat cortex

Salm

McCarthy

1989

Glia

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Astrocytes are the most numerous cell type in the central nervous system. When cultured, these cells express a wide variety of receptors for neurotransmitters. Nonetheless, it has yet to be firmly established that adult astrocytes in situ express receptors for neurotransmitters. In this report the technique of combined receptor autoradiography and immunocytochemistry has been applied to isolated astrocytes from adult animals to examine beta-adrenergic receptor (beta-AR) expression by these cells. It is shown that fibrous and protoplasmic astrocytes isolated from adult rat cerebral cortices specifically bind the beta-AR ligand iodocyanopindolol and that this binding is inhibited by the beta-AR ligands propranolol and isoproterenol. These results indicate that at least two of the major morphological subtypes of adult astrocytes express beta-ARs.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Expression of beta‐adrenergic receptors by astrocytes isolated from adult rat cortex

Salm

McCarthy

1989

Glia

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“…Although the rate of uptake after preincubation with insulin was markedly and significantly higher than controls, the absolute rates of uptake, even with insulin treatment, were much lower than we have found even in the T,depleted cells. It should be pointed out that a relatively long incubation time (5 min) was used for their uptake assays and that their cells were prepared using trypsin which results in loss of cell surface receptors (Henn et al, 1980) and reduced the rates of transport of 2-deoxyglucose (Roeder and Tildon, 1984).…”

Section: Discussionmentioning

confidence: 99%

Glucose Transport in Astrocytes: Regulation by Thyroid Hormone

1985

Journal of Neurochemistry

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Primary cultures of astrocytes from newborn rat brain showed evidence of a substrate-saturable process for glucose transport. The system shows a relatively high affinity for the substrate, with an apparent Km of approximately 1 mM. Maintenance of the cells in medium containing thyroid-hormone-free serum for 3, 6, or 9 days resulted in significantly reduced rates of hexose transport. Addition of exogenous triiodothyronine to the transport incubation medium of these "hypothyroid" cells markedly increased the net rate of 2-deoxyglucose uptake within 60 s to values equal to or above those of control cultures (cells maintained in normal serum). These findings support a key role for thyroid hormone in the transport of glucose across plasma membranes of brain cells and demonstrate the presence of this regulatory system in astrocytes.

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“…If the receptor-containing membrane fragments are neuronally derived, this could explain the different fractionation pattern of 5'-nucleotidase and radioligand binding activities observed in the present studies. Although ["Hlspiperone binding activity has been demonstrated in a glial cell fraction (Henn et al, 1980), the fraction used is unlikely to be pure, and a neuronal location of the radioligand binding activities is supported by the loss of [3H]-QNB and [3H]spiperone binding sites upon kainic acid lesioning of rat striatum (Fields et al, 1978). The microsomal preparation has also been fractionated by Mahler and coworkers (De Blas and Mahler, 1978;Near and Mahler.…”

Section: Discussionmentioning

confidence: 99%

The Distribution of Radioligand Binding Activity and 5′‐Nucleotidase Activity in Bovine Caudate Nucleus Subcellular Fractions

Withy

Mayer

Strange

1982

Journal of Neurochemistry

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The distribution of 5'-nucleotidase activity, dopaminergic [3H]spiperone binding sites, and [3H]quinuclidinyl benzilate (QNB) binding sites in different subcellular fractions of bovine caudate nucleus has been studied. Each activity was enriched in a microsomal (P3) preparation from that tissue. The microsomal preparation was further fractionated by different techniques. First, the P3 fraction, or a sonicated P3 fraction, was fractionated on a discontinuous sucrose density gradient. Second, the P3 fraction, or a digitonin pretreated P3 fraction, was fractionated on a continuous sucrose density gradient. The results obtained demonstrate that 5'-nucleotidase activity does not cofractionate with radioligand binding activity, although no difference between the distributions of [3H]spiperone binding and [3H]QNB binding were seen. It is concluded that the two radioligand binding activities are located on nonglial membranes.

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Receptor studies on isolated astroglial cell fractions prepared with and without trypsin

Cited by 26 publications

References 4 publications

Expression of beta‐adrenergic receptors by astrocytes isolated from adult rat cortex

Expression of beta‐adrenergic receptors by astrocytes isolated from adult rat cortex

Glucose Transport in Astrocytes: Regulation by Thyroid Hormone

The Distribution of Radioligand Binding Activity and 5′‐Nucleotidase Activity in Bovine Caudate Nucleus Subcellular Fractions

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