Receptors and growth-promoting effects of insulin and insulinlike growth factors on cells from bovine retinal capillaries and aorta.

King, George L.; Goodman, A. David; Buzney, Sheldon M.; Moses, Alan C.; Kahn, C. Ronald

doi:10.1172/jci111764

Cited by 419 publications

(184 citation statements)

References 27 publications

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“…Several lines of evidence, including in vitro studies, support the notion that IGF-1 is critical for vessel development (King et al, 1985;Grant et al, 1993). Preterm infants with reduced serum levels of IGF-1 have a higher incidence of development of retinopathy (Hellstrom et al, 2003).…”

Section: Regulation Of Vegf Expressionmentioning

confidence: 94%

Vascular endothelial growth factor in eye disease

Penn

Madan

Caldwell

et al. 2008

Progress in Retinal and Eye Research

636

527

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Collectively, angiogenic ocular conditions represent the leading cause of irreversible vision loss in developed countries. In the U.S., for example, retinopathy of prematurity, diabetic retinopathy and age-related macular degeneration are the principal causes of blindness in the infant, working age and elderly populations, respectively. Evidence suggests that vascular endothelial growth factor (VEGF), a 40 kDa dimeric glycoprotein, promotes angiogenesis in each of these conditions, making it a highly significant therapeutic target. However, VEGF is pleiotropic, affecting a broad spectrum of endothelial, neuronal and glial behaviors, and confounding the validity of anti-VEGF strategies, particularly under chronic disease conditions. In fact, among other functions VEGF can influence cell proliferation, cell migration, proteolysis, cell survival and vessel permeability in a wide variety of biological contexts. This article will describe the roles played by VEGF in the pathogenesis of retinopathy of prematurity, diabetic retinopathy and age-related macular degeneration. The potential disadvantages of inhibiting VEGF will be discussed, as will the rationales for targeting other VEGF-related modulators of angiogenesis.

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Section: Regulation Of Vegf Expressionmentioning

confidence: 94%

Vascular endothelial growth factor in eye disease

Penn

Madan

Caldwell

et al. 2008

Progress in Retinal and Eye Research

636

527

View full text Add to dashboard Cite

show abstract

“…Cell cultures. Primary cultures of porcine retinal endothelial cells (PRECs) were isolated by homogenization and a series of filtration steps as described (17,18). After the cells reached confluence, cells from passages 3 to 8 were used in this study.…”

Section: Methodsmentioning

confidence: 99%

Leptin Stimulates Ischemia-Induced Retinal Neovascularization

et al. 2004

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“…Vascular endothelial cells express insulin receptors as well as IGF-I receptors [21][22][23]. HUVECs contain approximately 3.5-10 times more IGF-I receptor mRNA than insulin receptor mRNA [24,25].…”

Section: Introductionmentioning

confidence: 99%

Insulin and its analogue glargine do not affect viability and proliferation of human coronary artery endothelial and smooth muscle cells

Staiger

Schweitzer³

et al. 2005

Diabetologia

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Aims/hypothesis: Present guidelines for the treatment of type 2 diabetes recommend HbA 1 c values of less than 7%. As beta cell function worsens during progress of the disease, insulin therapy is often necessary to achieve this ambitious goal. However, due to peripheral insulin resistance, many patients need rather high insulin dosages. In the light of the extremely high cardiovascular risk of diabetic patients, it is important to determine whether high concentrations of insulin or its frequently used analogues are harmful to the cardiovascular system. We therefore investigated the modulatory effects of regular human insulin and its analogue glargine on proliferation and apoptosis of human coronary artery endothelial cells (HCAECs) and human coronary artery smooth muscle cells (HCASMCs). Methods: Cells were treated with regular human insulin or insulin glargine. Proliferation was determined by [ 3 H]thymidine incorporation and by flow cytometric analysis of Ki-67 expression. Apoptosis was assessed by flow cytometry (cell cycle analysis and annexin V staining) and determination of caspase-3 activity. Results: HCAECs and HCASMCs treated with regular human insulin or insulin glargine did not show significant increases in DNA synthesis or Ki-67 expression. Administration of regular human insulin or insulin glargine did not modulate the extent of apoptotic events. No influence of insulin on lipoapoptotic vascular cell death could be detected.Conclusions/interpretation: Taken together, neither regular human insulin nor insulin glargine influences growth and apoptosis of human coronary artery cells in vitro. Our data do not suggest that regular human insulin or insulin glargine promote atherosclerosis through mechanisms affecting the cellularity of human coronary arteries.

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Receptors and growth-promoting effects of insulin and insulinlike growth factors on cells from bovine retinal capillaries and aorta.

Cited by 419 publications

References 27 publications

Vascular endothelial growth factor in eye disease

Vascular endothelial growth factor in eye disease

Leptin Stimulates Ischemia-Induced Retinal Neovascularization

Insulin and its analogue glargine do not affect viability and proliferation of human coronary artery endothelial and smooth muscle cells

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