Receptors for 1,25(OH)<sub>2</sub>‐vitamin D<sub>3</sub> enriched in cloned osteoblast‐like rat osteogenic sarcoma cells

Partridge, Nicola C.; Frampton, Richard; Eisman, John A.; Michelangeli, V. P.; Elms, EF; Bradley, T. R.; Martin, T. J.

doi:10.1016/0014-5793(80)80744-7

Cited by 165 publications

(46 citation statements)

References 18 publications

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“…This effect was mainly due to the presence of DEX and VD3 in the OM, rather than AA2P and β-GP, based on the observation that the MM group had a comparable cell number range with the CM group after switching the medium at week 3. A few studies have demonstrated an inhibitory effect of either DEX or vitamin D3 on cell proliferation (Walsh et al, 2001;Murata et al, 2004;Campbell et al, 1997), although they can induce osteogenic differentiation (Jaiswal et al, 1997;Partridge et al, 1980;Beresford et al, 1980). In monolayer culture, we also observed in a previous study that DEX inhibited hUCMSC proliferation (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering

Wang

Singh

Bonewald

et al. 2009

J Tissue Eng Regen Med

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Human umbilical cord mesenchymal stromal cells (hUCMSCs) have recently shown the capacity to differentiate into multiple cell lineages in all three embryonic germ layers. The osteogenic differentiation of hUCMSCs in monolayer culture has been reported, while the differentiation in three-dimensional biomaterials has not yet been reported for tissue-engineering applications. Thus, the aim of this study was to evaluate the feasibility of using hUCMSCs for bone tissue engineering. hUCMSCs were cultured in poly(L-lactic acid) (PLLA) scaffolds in osteogenic medium (OM) for 3 weeks, after which the scaffolds were exposed to several different media, including the OM, a mineralization medium (MM) and the MM with either 10 or 100 ng/ml insulin-like growth factor (IGF)-1. The osteogenic differentiation was confirmed by the up-regulation of Runx2 and OCN, calcium quantification and bone histology. Switching from the OM to the MM promoted collagen synthesis and calcium content per cell, while continuing in the OM retained more cells in the constructs and promoted higher osteogenic gene expression. The addition of IGF-1 into the MM had no effect on cell proliferation, differentiation and matrix synthesis. In conclusion, hUCMSCs show significant potential for bone tissue engineering and culturing in the OM throughout the entire period is beneficial for osteogenic differentiation of these cells.

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Section: Discussionmentioning

confidence: 99%

Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering

Wang

Singh

Bonewald

et al. 2009

J Tissue Eng Regen Med

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“…The demonstrations that the 24-hydroxylation pathway continues further with the formation of a side-chain cleaved molecule, a C23 alcohol Reddy et al, 1987;) provided a strong indicator that the 24-hydroxylation pathway would turn out to be the source of the intermediates leading to calcitroic acid. In this paper we report the formation of calcitroic acid from 1,25(OH)2D3 in two target cell systems in vitro and we provide evidence for the role of the 24-hydroxylation pathway in this metabolic process. A preliminary account of aspects of this work was presented to the 7th Workshop on Vitamin D, Rancho Mirage, California, U.S.A.; 24-29 April, 1988. EXPERIMENTAL Materials UMR-106 cells (Partridge et al, 1980) were obtained from J.N.M. Heersche, University of Toronto, Toronto, Canada.…”

Section: Introductionmentioning

confidence: 99%

Target cell metabolism of 1,25-dihydroxyvitamin D3 to calcitroic acid. Evidence for a pathway in kidney and bone involving 24-oxidation

Makin

Lohnes

Byford

et al. 1989

Biochemical Journal

232

114

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1,25-dihydroxyvitamin D3 is converted to calcitroic acid before being excreted in the bile. Biosynthesis of calcitroic acid has been demonstrated in two target cells of vitamin D, in the kidney and the osteoblastic cell line UMR-106. Calcitroic acid was identified by combinations of h.p.l.c., u.v. spectroscopy and mass spectrometry. Evidence is presented that calcitroate is derived from the 24-oxidation pathway, possibly through the intermediate 24,25,26,27-tetranor-1,23-dihydroxyvitamin D3. The 24-oxidation pathway to calcitroic acid in bone cells is stimulated by 1,25-dihydroxyvitamin D3. The pathway in both bone cells and perfused kidney operates at physiological concentrations of substrate and appears to be capable of rapid clearance of the hormone.

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“…Recently, it has been shown that the homeodomain-containing Msx-2 protein is expressed in osteoblastic cells (23). In addition, both a natural gene defect of msx-2 (24) and a null mutation of the msx-J locus (25) (27) were cultured in minimal essential medium (MEM; GIBCO) supplemented with 10% fetal calf serum. Primary cultures of normal diploid osteoblasts were derived from fetal rat calvaria and were maintained as described (28).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional control of the tissue-specific, developmentally regulated osteocalcin gene requires a binding motif for the Msx family of homeodomain proteins.

Hoffmann

Catron

Wijnen

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

120

106

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The OC box of the rat osteocalcin promoter (nt -99 to -76) is the principal proximal regulatory element contributing to both tissue-specific and developmental control of osteocalcin gene expression. The central motif of the OC box includes a perfect consensus DNA binding site for certain homeodomain proteins. Homeodomain proteins are transcription factors that direct proper development by regulating specific temporal and spatial patterns of gene expression. We therefore addressed the role of the homeodomain binding motif in the activity of the OC promoter. In this study, by the combined application of mutagenesis and site-specific protein recognition analysis, we examined interactions of ROS 17/2.8 osteosarcoma cell nuclear proteins and purified Msx-1 homeodomain protein with the OC box. We detected a series ofrelated specific protein-DNA interactions, a subset of which were inhibited by antibodies directed against the Msx-1 homeodomain but which also recognize the Msx-2 homeodomain. Our results show that the sequence requirements for binding the Msx-1 or Msx-2 homeodomain closely parallel those necessary for osteocalcin gene promoter activity in vivo. This functional relationship was demonstrated by transient expression in ROS 17/2.8 osteosarcoma cells of a series of osteocalcin promoter (nt -1097 to +24)-reporter gene constructs containing mutations within and flanking the homeodomain binding site of the OC box. Northern blot analysis of several bone-related cell types showed that all of the cells expressed msx-1, whereas msx-2 expression was restricted to cells transcribing osteocalcin. Taken together, our results suggest a role for Msx-1 and -2 or related homeodomain proteins in transcription of the osteocalcin gene.Osteocalcin is one of the major noncollagenous proteins associated with mineralized bone extracellular matrix (1). Expression of the osteocalcin gene is dependent on cell type and stage-specific phenotypic development in osteoblasts (2-4). The osteocalcin gene is transcriptionally activated in postproliferative cells and expression is enhanced by 1,25-dihydroxyvitamin D3 (vitamin D) at the onset of extracellular matrix mineralization both in vivo and in vitro (refs. 5-9 and reviewed in ref. 10). Transcription in bone cells has been defined by the activity of a modular promoter containing multiple independent sites that exhibit recognition for transcription factors that transduce and integrate a broad spectrum of physiological regulatory signals (8,9,(11)(12)(13)(14)(15).Deletion analysis has demonstrated that the principal basal regulatory domain of the proximal osteocalcin promoter is localized between nt -108 and +24 (11,16). We have previously shown that critical transcriptional control mechanisms are associated with recognition by cognate transcription factors of a 24-nt element designated the OC box (nt -99 to -76). In vivo competition experiments demonstrate that expression of an osteocalcin promoter-reporter gene construct is blocked when cotransfected into osteoblastic cells with...

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Receptors for 1,25(OH)₂‐vitamin D₃ enriched in cloned osteoblast‐like rat osteogenic sarcoma cells

Cited by 165 publications

References 18 publications

Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering

Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering

Target cell metabolism of 1,25-dihydroxyvitamin D3 to calcitroic acid. Evidence for a pathway in kidney and bone involving 24-oxidation

Transcriptional control of the tissue-specific, developmentally regulated osteocalcin gene requires a binding motif for the Msx family of homeodomain proteins.

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Receptors for 1,25(OH)2‐vitamin D3 enriched in cloned osteoblast‐like rat osteogenic sarcoma cells

Cited by 165 publications

References 18 publications

Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering

Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering

Target cell metabolism of 1,25-dihydroxyvitamin D3 to calcitroic acid. Evidence for a pathway in kidney and bone involving 24-oxidation

Transcriptional control of the tissue-specific, developmentally regulated osteocalcin gene requires a binding motif for the Msx family of homeodomain proteins.

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Receptors for 1,25(OH)₂‐vitamin D₃ enriched in cloned osteoblast‐like rat osteogenic sarcoma cells