Receptors for human gamma interferon: binding and crosslinking of 125I-labeled recombinant human gamma interferon to receptors on WISH cells.

Abstract

Purified recombinant human y interferon (HuIFN-y), labeled with 125I , was used to study receptors for HuIFN-y on human WISH cells. 1251_

The Human Interferon‐γ Receptor System

“…A radiolabelled band corresponding to molecular weight 120000 was obtained by SDS-PAGE when '"I-IFN-y was cross-linked to adherent cells such as HeLa, WISH or foreskin fibroblasts. This result confimied previous studies (Sarkar & Gupta 1984). When '"I-IFN-y was cross-linked to its receptor on peripheral blood monocytes, the 120000 band was not seen and, instead, a faint band of M.W.…”

Epidermal growth factor and transforming growth factor‐alpha decrease gamma interferon receptors and induction of intercellular adhesion molecule (ICAM‐1) on cultured keratinocytes

“…Using 17,000 dalton, biologically active 32P-labelled IFN-y, a single class of high affinity IFN-y receptors was demonstrated by Scatchard analysis to be present on multi-passaged human keratinocytes. The ligand binding studies performed at 4°C revealed a specific, time dependent (plateau at 4-6 hours), and saturatable reaction, with the resultant Kd and receptorsicell (0.25 nM;19.8 x lo3, respectively) to be within the range of values previously reported characterizing IFN-y receptors on cells such as keratinocytes, fibroblasts, WISH cells, and monocytei macrophages (Nickoloff, 1987;Mariano et al, 1987;Anderson et al, 1982;Finbloom et al, 1985;Sarkar and Gupta, 1984;Celada et al, 1984;Orchansky et al, 1986). Moreover, the current results indicate that the number of high affinity IFN-y receptor sitesicell, as detected by ligand binding studies, was dependent on the growth characteristics of the cultured keratinocytes.…”

Regulation of interferon receptor expression in human blood lymphocytes in vitro and during interferon therapy.

“…In vivo studies were done on PBL isolated from patients being treated with (20)(21)(22)(23)(24)(25) gl) were mixed with an equal volume of electrophoresis buffer (containing I mM dithiothreitol and 2-mercaptoethanol), boiled for 5 min, and subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) using 7.5% gels (18). Gels were dried under vacuum, and exposed to XAR film ( Cells were treated with IFN a2, and subsequent '25I-IFN a2 binding assays were performed as described in Fig.…”