Receptors for polymerized human serum albumin on hepatitis B virus particles detected by radioimmunoassay: Changes in receptor activity in serum during acute and chronic infection

Pontisso, Patrizia; Alberti, Alfredo; Schiavon, E; Tremolada, F.; Bortolotti, Flavia; Realdi, Giuseppe

doi:10.1016/0166-0934(83)90027-7

Cited by 17 publications

(10 citation statements)

References 9 publications

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“…Mice were immunized to determine the ED50 (median effective dose) in accord with OMS recommendations (17). (ii) Polyalbumin binding was also studied by solid phase RIA (19). Human serum albumin was polymerized by 2.5% glutaraldehyde treatment and the solution was then chromatographed on a Sephadex G-200 column in P1/NaCl.…”

Section: Methodsmentioning

confidence: 99%

Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin.

Michel¹,

Pontisso²,

Sobczak³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

173

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Section: Methodsmentioning

confidence: 99%

Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin.

Michel¹,

Pontisso²,

Sobczak³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

173

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“…After electrophoresis, the gel was fixed in 40% methanol and 10% acetic acid, dried, treated by PPO according to Bonner and Laskey (1974), and exposed to Kodak X-Omat R film at -70°C. pHSA receptor activity of HBsAg particles HBsAg receptors for pHSA were determined both by passive hemagglutination of sheep red blood cells (SRBC) coated with pHSA as described by Imai et al (1979) and by a solid-phase RIA as described by Hansson and Purcell (1979) with minor modifications (Pontisso et al, 1983a). Inoculation of rabbits New Zealand White female rabbits were inoculated i.v.…”

Section: Purification Of Hbsagmentioning

confidence: 99%

In vitro and in vivo synthesis of the hepatitis B virus surface antigen and of the receptor for polymerized human serum albumin from recombinant human adenoviruses.

Ballay¹,

Levrero²,

Buendía³

et al. 1985

The EMBO Journal

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We have developed an adenovirus vector to express foreign proteins under the control of the adenovirus Ela promoter. Two recombinant plasmids, harbouring either the S gene or the pre-S2 region and the S gene of hepatitis B virus under the control of the Ela promoter, were used to construct two recombinant adenoviruses. These two viruses direct the synthesis of hepatitis B virus surface antigen (HBsAg) particles during the time course of an infectious cycle. When the pre-S2 region is present in the constructed virus, the synthesis of particles carrying the receptor for polymerized human serum albumin (pHSA) is observed. Moreover, the inoculation of rabbits with this latter purified recombinant adenovirus elicits the production of antibodies that react with both HBsAg and pHSA receptor.

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“…Only humans and chimpanzees can be infected with HBV because only they have poly-HSA receptors (8). The clinical significance of poly-HSA receptors has been reported by several authors (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20). According to these reports, measurements of pre-S2 Ag in clinical cases are useful in determining the activation and infectivity of HBsAg carriers.…”

Section: Discussionmentioning

confidence: 95%

Significance of measurement of pre‐S2 antigen for the prevention of vertical transmission of hepatitis B virus in infants born to HBsAg carrier mothers

1994

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The significance of pre-S2 antigen (pre-S2 Ag) as a marker of hepatitis B virus (HBV) infection, especially in infants born to HBsAg carrier mothers who are HBeAg-negative or HBeAg-positive, was evaluated. Pre-S2 Ag was measured by enzyme immunoassay. HBsAg carrier mothers who were HBeAg-negative and HBeAb-positive were divided into two groups: group A, mothers whose infants were not infected with HBV (n = 10) and group B, mothers whose infants were infected with HBV (n = 13). Absorption rates of pre-S2 Ag in group A and B were 0.09 +/- 0.04 and 1.36 +/- 0.95, respectively. The values for pre-S2 Ag in group B were significantly higher than those in group A. Values for pre-S2 Ag among HBsAg carrier mothers who were HBeAg-positive and HBeAb-negative were also measured by reverse passive hemagglutination. In the same way, HBsAg carrier mothers who were HBeAg-positive and HBeAb-negative were divided into two groups: group C, mothers whose infants did not become HBsAg carriers (n = 15) and group D, mothers whose infants became HBsAg carriers (n = 11). The titers of pre-S2 Ag (reverse passive hemagglutination) in group C and D were 2(5.75) +/- 1.68 and 2(10.45 +/- 1.69), respectively. The values for pre-S2 Ag in group D were significantly higher than those in group C. The values for pre-S2 Ag as markers of infectivity became higher with increasing amounts of HBV-DNA.(ABSTRACT TRUNCATED AT 250 WORDS)

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Receptors for polymerized human serum albumin on hepatitis B virus particles detected by radioimmunoassay: Changes in receptor activity in serum during acute and chronic infection

Cited by 17 publications

References 9 publications

Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin.

Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin.

In vitro and in vivo synthesis of the hepatitis B virus surface antigen and of the receptor for polymerized human serum albumin from recombinant human adenoviruses.

Significance of measurement of pre‐S2 antigen for the prevention of vertical transmission of hepatitis B virus in infants born to HBsAg carrier mothers

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