Recessive resistance genes against potyviruses are localized in colinear genomic regions of the tomato (Lycopersicon spp.) and pepper (Capsicum spp.) genomes

Parrella, G.; Ruffel, Sandrine; Moretti, A.; Morel, Caroline; Palloix, Alain; Caranta, Carole

doi:10.1007/s00122-002-1005-2

Cited by 78 publications

(47 citation statements)

References 39 publications

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“…This is putatively explained by the rapid evolution of major R loci involved in a resistance trait. In contrast, recessive determinants of resistance against potyviruses are co-linear in tomato and pepper genomes (Parrella et al 2002). Therefore, functionally conserved synteny between apricot and P. davidiana may indicate that this locus is distinct from major R genes.…”

Section: Discussionmentioning

confidence: 98%

Analogues of virus resistance genes map to QTLs for resistance to sharka disease in Prunus davidiana

et al. 2005

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Plum pox virus (PPV), the causative agent of sharka disease in Prunoideae, is one of the most serious problems affecting stone fruit production in Europe and America. Resistance to PPV was previously described in a Prunus davidiana clone, P1908, and introduced into peach (Prunus persica) genotypes. Genetic resistance to PPV displays a complex pattern of quantitative inheritance. An analysis of quantitative trait loci (QTLs) for resistance was performed on an F1 interspecific peach population obtained from a cross between the susceptible nectarine cultivar Summergrand and P. davidiana. The hybrids were graft-inoculated with PPV in duplicate following a classical procedure. The incidence of infection was evaluated four times, over two vegetative cycles, by symptom observation and enzyme-linked immunoadsorbent assays (ELISA). Restriction of systemic downward movement of the PPV virus was also evaluated by testing the susceptible rootstocks. Using both analysis of variance and non-parametric tests, six genomic regions involved in PPV resistance were detected. Depending on the scoring data considered, between 22 and 51% of the phenotypic variance could be explained by the quantitative model. One QTL, located in the distal region of linkage group 1, maps in a genomic region that is syntenic to the location of a resistance gene previously identified in the apricot cv. Goldrich. Some QTLs appeared to be temporally specific, reflecting the environmental dependence of PPV-resistance scoring. Candidate gene fragments were amplified by PCR, isolated and mapped on the peach interspecific linkage map. We report here the co-localization of three analogues of virus resistance genes with two distinct genomic regions linked to PPV resistance in P. davidiana.

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Section: Discussionmentioning

confidence: 98%

Analogues of virus resistance genes map to QTLs for resistance to sharka disease in Prunus davidiana

et al. 2005

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“…The Lt-6.1 QTL, linked to GC002, is also linked to CD025 and CT204 (Lefebvre et al 1998), and is likely to correspond to the Ol-1/Ol-3 loci tomato cluster. Colinearity of loci governing resistance to identical or similar pathogens was rarely described in the literature (Djian-Caporalino et al 2001;Parrella et al 2002;Thabuis et al 2003). Functional orthology among resistance loci occurring in several species could be attributed to a common ancestral gene.…”

Section: Discussionmentioning

confidence: 99%

QTLs for resistance to powdery mildew in pepper under natural and artificial infections

et al. 2003

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Epidemics of powdery mildew due to Leveillula taurica is an increasing problem in pepper production areas, particularly in coastal regions or greenhouse cultivation. The highly resistant genitor 'H3' was submitted to genetic analysis and QTL mapping in order to promote the introgression of its oligogenic resistance into large and sweet-fruited cultivars. The doubled-haploid progeny from the cross 'H3' (resistant) by 'Vania' (susceptible) was tested for resistance under both natural field infection and artificial inoculation tests, and QTL detection was compared for those two methods. Seven genomic regions including additive QTLs and epistatic interactions were detected, explaining altogether the major part of genotypic variance. Two genomic regions were common to both the evaluation methods, whereas other QTLs were method-specific, reflecting the environment dependence of powdery mildew epidemics. Orthologies with tomato genomic regions carrying resistance genes to L. taurica and Oidium lycopersicum were revealed by comparative mapping with pepper. Tight linkages between the detected QTLs and virus resistance or fruit color traits in pepper were also shown, which adds to the agronomic importance of these regions in pepper breeding programs.

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“…All except PI24 are susceptible to PVY and TEV; PI24 contains the recessive pot-1 allele for resistance to PVY and TEV (Parrella et al 2002).…”

Section: Methodsmentioning

confidence: 99%

“…pot-1 + /pot-1 + ) or homozygous resistant (i.e. pot-1/pot-1) based on phenotypic evaluation of F2-derived F3 families (as previously described by Parrella et al 2002) were used for analysis of genetic segregation between the pot-1 and eIF4E loci.…”

Section: Methodsmentioning

confidence: 99%

“…In comparison with resistance genes controlling other pathogens, recessive resistance to potyviruses is relatively common, comprising about half of all known resistances against this viral genus (DiazPendon et al 2004). Comparative genetic studies of resistance to potyviruses between tomato and pepper have shown that the resistance genes pot-1 and pvr2 are localized in colinear regions of the tomato and pepper genomes (Parrella et al 2002). Both genes control a complete resistance to the Potato virus Y (PVY) and the Tobacco etch virus (TEV) via a mechanism that prevents virus accumulation in inoculated tissues.…”

Section: Introductionmentioning

confidence: 99%

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The recessive potyvirus resistance gene pot-1 is the tomato orthologue of the pepper pvr2-eIF4E gene

Ruffel¹,

Gallois²,

Lesage³

et al. 2005

Mol Genet Genomics

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The translation initiation factor 4E (eIF4E) has been implicated in naturally occurring resistance to Potato virus Y (PVY) determined by the pvr2 locus in pepper (Capsicum annuum). Here, the molecular basis of the recessive resistance to PVY and Tobacco etch virus (TEV) controlled by the pot-1 locus in tomato (Lycopersicon esculentum; now Solanum lycopersicum) was investigated. On the basis of genetic mapping data that indicated that pot-1 and pvr2 are located in syntenic regions of the tomato and pepper genomes, the possible involvement of eIF4E in pot-1-mediated resistance was assessed. Genetic mapping of members of the eIF4E multigenic family in tomato introgression lines revealed that an eIF4E locus indeed maps in the same genomic region as pot-1. By comparing eIF4E coding sequences between resistant and susceptible Lycopersicon genotypes, a small number of polymorphisms that co-segregate with the pot-1 locus were identified, suggesting that this gene could be involved in resistance to potyviruses. Functional complementation experiments using Potato virus X-mediated transient expression of eIF4E from a susceptible genotype in a resistant pepper genotype confirmed that a small number of amino acid substitutions in the eIF4E protein indeed account for resistance/susceptibility to both the PVY and TEV, and consequently that pot-1 and pvr2 are orthologues. Taken together, these results support the role of this eIF4E gene as a key component of recessive resistance to potyviruses, and validate the comparative genomic approach for the molecular characterization of recessive resistance genes.

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Recessive resistance genes against potyviruses are localized in colinear genomic regions of the tomato (Lycopersicon spp.) and pepper (Capsicum spp.) genomes

Cited by 78 publications

References 39 publications

Analogues of virus resistance genes map to QTLs for resistance to sharka disease in Prunus davidiana

Analogues of virus resistance genes map to QTLs for resistance to sharka disease in Prunus davidiana

QTLs for resistance to powdery mildew in pepper under natural and artificial infections

The recessive potyvirus resistance gene pot-1 is the tomato orthologue of the pepper pvr2-eIF4E gene

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