Rechargeable Afterglow Nanotorches for In Vivo Tracing of Cell‐Based Microrobots

Abstract

As one of the self‐luminescence imaging approaches that require pre‐illumination instead of real‐time light excitation, afterglow luminescence imaging has attracted increasing enthusiasm to circumvent tissue autofluorescence. In this work, we developed organic afterglow luminescent nanoprobe (nanotorch), which could emit persistent luminescence more than 10 days upon single light excitation. More importantly, the nanotorch could be remote charged by 660 nm light in a non‐invasive manner, which showed great potential for real‐time tracing the location of macrophage cell‐based microrobots.

Methylene Blue: An FDA-Approved NIR-II Fluorogenic Probe with Extremely Low pH Responsibility for Hyperchlorhydria Imaging

“…As an FDA (Food and Drug Administration)-approved drug, methylene blue has been used as a NIR contrast agent for the evaluation of the renal function of the animal or the activatable NIR probe by adding a carbamate caging group on the 10-N position. − In this work, we found that methylene blue could quickly be transformed into NIR-II emissive AIEgens through the protonation of methylene blue in extreme acidic solution. Moreover, the NIR-II emissive AIEgens could be generated in situ in a hyperchlorhydria stomach, which could be used for studying hyperchlorhydria related disease.…”