Recherche de l'activité pectionolytique chez 22 souches de champignons microscopiques isolées d'un sol de la region d'El-Kala

Abstract

22 microscopic fungi strains pertaining to the following genus : Aspergillus, Penicillium, Mucor, Alternaria, Nigrospora and Trichoderma have been isolated from soil in the region of El Kala (North-East of Algeria). These species present a depolymerising pectinolytic activity. They secrete polygalacturonases capable of hydrolysing pectic acid more or less esterified. Besides the strains studied, Trichoderma specie seems to be more active and secretes a polygalacturonase actived by chloride sodium (NaCl), acting at pH 5, at 30°C and its preferential substrate is polygalacturonic acid.

Partial purification and Characterization of Two Pectinases (Polygalacturonase and Pectin lyase) from Papaya Pericarp (Carica papaya cv. solo 8)

“…The optimum pH of our findings was similar to the pectinase of Penicillium varidictum RFC3 and Aspergillus niger (Silva et al, 2002;Fahmy et al, 2008), respectively. The range of pH at which polygalacturonase activity is maximal is between pH 3.5 and 6.0 (Fenghour et al, 2002;Amande et al, 2013). Polygalacturonase optimum pH was higher than that of purified polygalacturonase tomato (pH 4.4) (Verlent et al, 2004) and lower than polygalacturonase of kiwi (pH 5.5) (Shouqing, 2014), Aspergillus niger and Aspergillus flavus (pH 5.5) (Deshmukh et al, 2012) and Bacillus sp.…”