Recherches Expérimentales Sur La Maturation in Vitro Des Ovocytes De Truie Et De Veau

Foote, W. D.; Thibault, Christelle; Gérard, Micheline; Mélières, Anne-Marie

doi:10.1051/rnd:19690303

Cited by 129 publications

(54 citation statements)

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“…When oocytes reach the critical size during culture, they are able to resume meiosis spontaneously. However, it is interesting to note that after 2 to 10 days of culture on fibroblast monolayer middle-size oocytes also resume meiosis spontaneously (Canipari et al, 1984) showing Thibault, 1977 Thibault, 1969). These conclusions were followed by numerous attempts to isolate the inhibitor and to determine its chemical nature.…”

mentioning

confidence: 99%

Mammalian oocyte maturation

Thibault¹,

Szöllősi²,

Gérard³

1987

Reprod. Nutr. Dévelop.

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207

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mentioning

confidence: 99%

Mammalian oocyte maturation

Thibault¹,

Szöllősi²,

Gérard³

1987

Reprod. Nutr. Dévelop.

Self Cite

207

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“…Multidirectional communication between follicular cells is essential for oocyte maturation and competence [1]. This was clearly demonstrated by the work of Foote and Thibault [2] as early as 1969: premature disjunction between granulosa cells and the oocyte-cumulus complex prevents full oocyte competence; full competence requires a time-dependent sequence of events. However, the pathways through which hormonal and paracrine exchanges exert their effects on Capsule A sequential stimulation protocol (hFSH + recFSH ) has been compared to homogenous h FSH and rFSH protocol in three group of 30 patients.…”

Section: Introductionmentioning

confidence: 99%

Sequential (hFSH + recFSH) vs homogenous (hFSH or recFSH alone) stimulation: clinical and biochemical (cumulus cell gene expression) aspects

Gürgan

Montjean²,

Ménézo

2014

J Assist Reprod Genet

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FSH is a key hormone in the regulation of follicular development. Together with the EGF network, these molecules mediate oocyte maturation and competence in preparation for the action of LH. FSH isoforms regulate distinct biological pathways and have specific effects on granulosa cell function and maturation of the ovarian follicle. Their dynamic interactions occur during the follicular cycle; shortliving forms are predominant in the pre-ovulatory phase, whereas long-acting molecules characterize the lutealfollicular transition. Recombinant FSH (rFSH) molecules have a reduced number of isoforms and are less acidic, with a shorter half-life. We have investigated sequential stimulation, comparing hFSH + rFSH, vs. rFSH alone and hFSH alone for the entire stimulation phase. Sequential stimulation leads to an E2 per MII oocyte ratio that is much lower than is seen during treatment with the two drugs individually. Although there is a positive tendency in favor of the sequential treatment, there was no significant difference in pregnancy rates, even taking frozen embryos into consideration. The cumulus cell transcriptome varies considerably between the treatments, although with no clear significance. When comparing pregnant vs. non-pregnant patients, in general a decrease in mRNA expression can be observed in the pregnant patients, especially in expression of folic acid receptor 1 and ovostatin 2. This indicates that material has been transferred from CC to the oocyte. However, a common observation in the literature is that variations in the transcriptome of the cumulus cells are highly dependent upon the patient genotype; the potential for applying this strategy as a basis for selecting embryos is, at the very least, questionable.

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“…The major aim of this study was to test whether the meiosis-like changes during atresia were accompanied by similar changes in respiration. It was found that oocyte respiration in atretic follicles followed the same pattern as during normal maturation (Magnusson et al, 1977) Gonadotrophins have been shown to inhibit cumulus respiration (Dekel et al, 1976;Magnusson & Hillensjö, 1981 (Braw & Tsafriri, 1980;Uilenbroek et al, 1980; (Foote & Thibault, 1969;Tsafriri & Channing, 1975) is interrupted by the atretic degeneration. This would permit resumption of oocyte meiosis in a manner similar to normal maturation until the degenerative process affects the oocyte as well.…”

Section: Discussionmentioning

confidence: 99%