Reciprocal translocation identified in Vigna angularis dominates the wild population in East Japan

Wang, Lixia; Kikuchi, Shinichi; Muto, Chiaki; Naito, Kunihiko; Isemura, Takehisa; Ishimoto, Masao; Cheng, Xuzhen; Kaga, Akito; Tomooka, Norihiko

doi:10.1007/s10265-015-0720-0

Cited by 18 publications

(15 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The translocation was reciprocal-translocation where LG4 and LG6 were intermingled. This translocation was found in many accessions of wild azuki bean and it possibly affected fitness of those wild accessions to some environments and seed size [47]. In our study, comparative linkage analysis revealed macro-translocation between our zombi pea linkage map and the zombi pea linkage map developed by Marubodee et al [33]; a part of LG10 of the latter translocated to LG7 of the former (Fig 3).…”

Section: Discussionsupporting

confidence: 63%

See 1 more Smart Citation

QTL Analysis of Domestication Syndrome in Zombi Pea (Vigna vexillata), an Underutilized Legume Crop

Dachapak

Tomooka

Somta

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

2 Zombi pea (Vigna vexillata (L.) A. Rich) is an underutilized crop belonging to the genus Vigna. 3 Two domesticated forms of zombi pea are cultivated as crop plants; seed and tuber forms. The 4 cultivated seed form is present in Africa, while the cultivated tuber form is present in a very 5 limited part of Asia. Genetics of domestication have been investigated in most of cultivated 6Vigna crops by means of quantitative trait locus (QTL) mapping. In this study, we investigated 7 genetics of domestication in zombi pea by QTL analysis using an F 2 population of 139 plants 8 derived from a cross between cultivated tuber form of V. vexillata (JP235863) and wild V. 9 vexillata (AusTRCF66514). A linkage map with 11 linkage groups was constructed from this F 2 10 population using 145 SSR, 117 RAD-seq and 2 morphological markers. Many highly segregation 11 distorted markers were found on LGs 5, 6, 7, 8, 10 and 11. Most of the distorted markers were 12 clustered together and all the markers on LG8 were highly distorted markers. Comparing this V. 13 vexillata linkage map with a previous linkage map of V. vexillata and linkage maps of other four 14 Vigna species demonstrated several macro translocations in V. vexillata. QTL analysis for 22 15 domestication-related traits was investigated by inclusive composite interval mapping in which 16 37 QTLs were identified for 18 traits; no QTL was detected for 4 traits. Number of QTLs 17 detected in each trait ranged from 1 to 5 with an average of only 2.3. Tuber traits were controlled 18 by five QTLs with similar effect locating on different linkage groups. Large-effect QTLs (PVE > 19 20%) were on LG4 (pod length), LG5 (leaf size and seed thickness), and LG7 (for seed-related 20 traits). Comparison of domestication-related QTLs of the zombi pea with those of cowpea (Vigna 21 unguiculata), azuki bean (Vigna angularis), mungbean (Vigna radiata) and rice bean (Vigna 22 umbellata) revealed that there was conservation of some QTLs for seed size, pod size and leaf 23 size between zombi pea and cowpea and that QTLs associated with seed size (weight, length, 24 width and thickness) in each species were clustered on same linkage. 25 26 65 Knowledge on genetic basis of crop domestication is useful for identification of 66 beneficial gene(s) in the wild relatives of the cultivated crops that can be used in plant breeding. 67 Moreover, such knowledge can also provide insight into crop evolution and agriculture, such that 68 the case of genetic analysis of indehiscent pod in soybean by Funastuki [14] which showed 69 crucial role of the trait in the global expansion of soybean cultivation. Generally, genes 70 controlling crop domestication are identified by quantitative trait loci (QTL) analysis. Among 71 crops of the genus Vigna, QTL mappings of domestication syndrome traits have been carried out 72 in azuki bean [13,15], mungbean [16], rice bean [17], cowpea/yardlong bean [18-21]. The 73 genetics of domestication of zombi pea is of particular interest because two cultivated forms of 74 this leg...

show abstract

Section: Discussionsupporting

confidence: 63%

“…In addition, a part of the LG7 appeared to be duplication of LG2 or vice versa (Fig 3). Intraspecific macro-translocation has been demonstrated in azuki bean by means of comparative linkage mapping and fluorescence in situ hybridization [46,47]. The translocation was reciprocal-translocation where LG4 and LG6 were intermingled.…”

Section: Discussionmentioning

confidence: 99%

QTL Analysis of Domestication Syndrome in Zombi Pea (Vigna vexillata), an Underutilized Legume Crop

Dachapak

Tomooka

Somta

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…angularis ), we also suggested reciprocal translocation between LG4 and LG6 based on the comparison of SSR-based linkage maps developed for 2 different mapping populations [ 29 , 30 ]. Recently, this translocation event was confirmed to be occurred on a Japanese wild azuki bean parent by BAC-fluorescence in situ hybridization (FISH) analyses [ 62 ]. In that study, 21 wild azuki bean accessions collected from various regions of Japan were analyzed and it was found that geographical distribution of accessions with translocated chromosomes were restricted to eastern and northern Japan.…”

Section: Discussionmentioning

confidence: 99%

Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich

et al. 2015

Self Cite

View full text Add to dashboard Cite

Vigna vexillata (L.) A. Rich. (tuber cowpea) is an underutilized crop for consuming its tuber and mature seeds. Wild form of V. vexillata is a pan-tropical perennial herbaceous plant which has been used by local people as a food. Wild V. vexillata has also been considered as useful gene(s) source for V. unguiculata (cowpea), since it was reported to have various resistance gene(s) for insects and diseases of cowpea. To exploit the potential of V. vexillata, an SSR-based linkage map of V. vexillata was developed. A total of 874 SSR markers successfully amplified single DNA fragment in V. vexillata among 1,336 SSR markers developed from Vigna angularis (azuki bean), V. unguiculata and Phaseolus vulgaris (common bean). An F2 population of 300 plants derived from a cross between salt resistant (V1) and susceptible (V5) accessions was used for mapping. A genetic linkage map was constructed using 82 polymorphic SSR markers loci, which could be assigned to 11 linkage groups spanning 511.5 cM in length with a mean distance of 7.2 cM between adjacent markers. To develop higher density molecular linkage map and to confirm SSR markers position in a linkage map, RAD markers were developed and a combined SSR and RAD markers linkage map of V. vexillata was constructed. A total of 559 (84 SSR and 475 RAD) markers loci could be assigned to 11 linkage groups spanning 973.9 cM in length with a mean distance of 1.8 cM between adjacent markers. Linkage and genetic position of all SSR markers in an SSR linkage map were confirmed. When an SSR genetic linkage map of V. vexillata was compared with those of V. radiata and V. unguiculata, it was suggested that the structure of V. vexillata chromosome was considerably differentiated. This map is the first SSR and RAD marker-based V. vexillata linkage map which can be used for the mapping of useful traits.

show abstract

“…The BAC library construction and screening were performed as described by Wang et al (2015). The DNA markers “38930_set1” and ”39010_set1” were used for screening (Supplemental Table 2).…”

Section: Methodsmentioning

confidence: 99%

Multiple organ gigantism caused by mutation in <i>VmPPD</i> gene in blackgram (<i>Vigna mungo</i>)

et al. 2017

Self Cite

View full text Add to dashboard Cite

Seed size is one of the most important traits in leguminous crops. We obtained a recessive mutant of blackgram that had greatly enlarged leaves, stems and seeds. The mutant produced 100% bigger leaves, 50% more biomass and 70% larger seeds though it produced 40% less number of seeds. We designated the mutant as multiple-organ-gigantism (mog) and found the mog phenotype was due to increase in cell numbers but not in cell size. We also found the mog mutant showed a rippled leaf (rl) phenotype, which was probably caused by a pleiotropic effect of the mutation. We performed a map-based cloning and successfully identified an 8 bp deletion in the coding sequence of VmPPD gene, an orthologue of Arabidopsis PEAPOD (PPD) that regulates arrest of cell divisions in meristematic cells. We found no other mutations in the neighboring genes between the mutant and the wild type. We also knocked down GmPPD genes and reproduced both the mog and rl phenotypes in soybean. Controlling PPD genes to produce the mog phenotype is highly valuable for breeding since larger seed size could directly increase the commercial values of grain legumes.

show abstract

Reciprocal translocation identified in Vigna angularis dominates the wild population in East Japan

Cited by 18 publications

References 25 publications

QTL Analysis of Domestication Syndrome in Zombi Pea (Vigna vexillata), an Underutilized Legume Crop

QTL Analysis of Domestication Syndrome in Zombi Pea (Vigna vexillata), an Underutilized Legume Crop

Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich

Multiple organ gigantism caused by mutation in <i>VmPPD</i> gene in blackgram (<i>Vigna mungo</i>)

Contact Info

Product

Resources

About