benzoyl-1-deacetylmekongensine, and 7-epi-euojaponine A. Mekongensine and 9'-deacetoxymekongensine have very weak cytotoxicity to human oral epidermal carcinoma (KB) cell line but no cytotoxicity to human breast adenocarcinoma (MCF7) and human small cell lung (NCI-H187) cell lines. 7 This plant is not widely used for medicinal purposes and there have been no reports on immuneassociated properties. Macrophages defend against foreign antigens in the innate immune response. 8 Macrophages recognize microbial molecules by different pattern recognition receptors (PRRs) in response to infection and injury. 9 The mechanisms of macrophage action against microbes involve phagocytosis, stimulating inflammation, and presenting antigens. 8 However, immunomodulation effects of M. mekongensis extracts on human macrophages are still unclear. This research aims to study the phytochemical constituents of crude extracts from three parts of M. mekongensis, determine antioxidant activity, cytotoxicity on human red blood cells (RBC) and white blood cells (WBC), and immunomodulation activities of crude extracts on human macrophages.
Materials and MethodsPlant sample crude extracts preparation M. mekongensis In Sched. (Figure 1A) was collected in December 2019 from the saline soil at Ban Phonsim, Hua Na Kham Sub-district, Yang Talat District, Kalasin Province, at 16°24'01.8"N; 103°16'13.1"E. Professor Khwanruan Naksuwankul (Ph.D.) from the Faculty of Science, Mahasarakham University kindly identified the plant according to the Flora of Thailand manual. 10 Voucher specimens were deposited under the code number MSUT7444 at the Natural